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Effect of laparotomy and CO2 pneumoperitoneum on tumor growth of human colon carcinoma and expression pattern of tumor-associated proteins in the SCID mouse

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International Journal of Colorectal Disease Aims and scope Submit manuscript

Abstract

Background and aims

The impact of laparoscopy on tumor progression is still unclear. This study investigated the effect of CO2 pneumoperitoneum on the intra-abdominal growth of human colon carcinoma independently of the effect of the immune system.

Methods

SCID mice underwent either median laparotomy or laparoscopy. Human colon carcinoma cells were implanted into the upper abdomen. The control group was not operated on following cell injection. Tumor growth and the protein expression pattern of proliferation marker Ki67, cell-cell adhesion molecules E-cadherin, α- and β-catenin, and cell-extracellular matrix adhesion molecules CD44 v5 and v6 in tumor tissue were analyzed on postoperative day 14.

Results

Total tumor volume in the laparoscopy group significantly exceeded that in the laparotomy group. Immunohistochemistry revealed reduced expression of α-catenin and elevated expression on β-catenin and CD44 v5 in the tumor tissue of the laparoscopy group.

Conclusion

The expression pattern of proteins associated with tumor progression and the increase in tumor growth suggest an increased risk of laparoscopy at least for the growth of advanced human colon carcinoma.

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Authors and Affiliations

  1. Department of General Surgery, Georg August University, Robert-Koch-Strasse 40, 37075, Göttingen, Germany

    I. Leister, S. Manegold, P. Schüler, H. Becker & P. M. Markus

  2. Department of Hematology and Oncology, Georg August University, Robert-Koch-Strasse 40, 37075, Göttingen, Germany

    F. Alves

  3. Department of Pathology, Georg August University, Robert-Koch-Strasse 40, 37075, Göttingen, Germany

    L. Füzesi

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  1. I. Leister
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  2. S. Manegold
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  3. P. Schüler
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Correspondence to I. Leister.

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Leister, I., Manegold, S., Schüler, P. et al. Effect of laparotomy and CO2 pneumoperitoneum on tumor growth of human colon carcinoma and expression pattern of tumor-associated proteins in the SCID mouse. Int J Colorectal Dis 18, 508–513 (2003). https://doi.org/10.1007/s00384-003-0486-z

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