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Nutritional supplementation with transforming growth factor-beta inhibits intestinal adaptation after massive small bowel resection in a rat

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Abstract

Introduction

Transforming growth factor beta (TGF-β) has been shown to affect epithelial cell differentiation and proliferation through epithelial-mesenchymal and epithelial-immune cell interaction. In the present study, we evaluated the effect of TGF-β2-enriched polymeric diet (Modulen) on enterocyte turnover in a rat model of short bowel syndrome (SBS).

Methods

Male rats were divided into four groups: Sham rats and Sham-TGF-β rats underwent bowel transection, and were treated with TGF-β from the 4th postoperative day, SBS rats underwent a 75% bowel resection, and SBS-TGF-β rats underwent bowel resection and were treated with TGF-β-enriched diet similar to Group B. Parameters of intestinal adaptation, enterocyte proliferation and apoptosis were determined on day 15. Real-time PCR was used to determine Bax and Bcl-2 mRNA expression.

Results

Treatment of SBS animals with TGF-β2 supplemented diet led to a significant decrease (vs. SBS rats) in bowel weight in ileum (18%, P < 0.05), mucosal DNA content in jejunum (threefold decrease, P < 0.05) and ileum (2.5-fold decrease, P < 0.05), and mucosal protein in jejunum (twofold decrease, P < 0.05) compared to SBS-untreated animals (Group B). Treatment with TGF-β resulted in a mild decrease in enterocyte proliferation in jejunum (25%, P < 0.05) and ileum (18%, P < 0.05). A decreased cell apoptosis in the SBS-TGF-β group was accompanied by a decreased Bax and increased Bcl-2 mRNA expression.

Conclusions

In a rat model of SBS, dietary TGF-β inhibits intestinal adaptation. Decreased enterocyte proliferation is responsible for this effect.

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Acknowledgments

This work was supported by an Israel Science Foundation (ISF) research grant (No 1135/08).

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Authors and Affiliations

  1. Laboratory of Intestinal Adaptation and Recovery, The Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel

    Igor Sukhotnik, Shani Ben Lulu & Yulia Bashenko

  2. Department of Pediatric Surgery B, Bnai Zion Medical Center, 47 Golomb St., P.O.B 4940, 31048, Haifa, Israel

    Igor Sukhotnik & Jorge G. Mogilner

  3. Department of Pathology, Bnai Zion Medical Center, Haifa, Israel

    Elena Chemodanov

  4. Pediatric Gastroenterology and Nutrition Unit, Meyer Children’s Hospital, Rambam Medical Center, Haifa, Israel

    Ron Shaoul

  5. Section of Pediatric Surgery, C.S. Mott Children’s Hospital and University of Michigan Medical School, Ann Arbor, MI, USA

    Arnold G. Coran

Authors
  1. Igor Sukhotnik
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  2. Jorge G. Mogilner
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  3. Shani Ben Lulu
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  4. Yulia Bashenko
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  5. Ron Shaoul
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  6. Elena Chemodanov
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  7. Arnold G. Coran
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Corresponding author

Correspondence to Igor Sukhotnik.

Additional information

I. Sukhotnik and J.G. Mogilner equally contributed to preparation of the manuscript.

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Sukhotnik, I., Mogilner, J.G., Ben Lulu, S. et al. Nutritional supplementation with transforming growth factor-beta inhibits intestinal adaptation after massive small bowel resection in a rat. Pediatr Surg Int 27, 211–216 (2011). https://doi.org/10.1007/s00383-010-2774-3

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  • DOI: https://doi.org/10.1007/s00383-010-2774-3

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