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Nerve growth factor receptor immunohistochemistry has a limited additional value to diagnose Hirschsprung’s disease

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Abstract

Purpose

The diagnosis of Hirschsprung’s disease (HSCR) is based on the histopathological evaluation of rectal suction biopsies (RSB), using haematoxylin and eosin (H&E) stains and acetylcholinesterase (AChE) histochemistry. The use of different immunohistochemical markers, such as nerve growth factor receptor (NGFR), has been suggested to facilitate the diagnosis of HSCR. The aim of this study was to evaluate the addition of NGFR immunohistochemistry to diagnose HSCR.

Methods

RSB from 23 HSCR patients and 16 patients investigated for, but not diagnosed with, HSCR were retrospectively reviewed. The histopathology report supported or did not support the diagnosis of HSCR.

Results

In patients with HSCR, the primary biopsies confirmed the diagnosis in 21 of 23 cases with H&E staining, in 16 of 23 cases with AChE histochemistry, and in 8 of 23 cases with NGFR immunohistochemistry. Due to inadequate biopsies or equivocal interpretation, the biopsies were repeated in seven of the patients with HSCR and two patients underwent biopsies a third time. In the 16 patients investigated for but not diagnosed with HSCR, the three tests were normal in all cases.

Conclusion

We conclude that NGFR immunohistochemistry has limited additional value to diagnose HSCR.

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Authors and Affiliations

  1. Division of Pediatric Surgery, Department of Women’s and Children’s Health, Karolinska Institute, Stockholm, Sweden

    Anna Löf Granström, Pär-Johan Svensson, Markus Almström & Tomas Wester

  2. Department of Pathology and Cytology, Karolinska University Hospital, Stockholm, Sweden

    Abiel Orrego & Jurate Skikuniene

Authors
  1. Anna Löf Granström
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  2. Abiel Orrego
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  3. Pär-Johan Svensson
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  4. Markus Almström
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  5. Jurate Skikuniene
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  6. Tomas Wester
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Corresponding author

Correspondence to Anna Löf Granström.

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Granström, A.L., Orrego, A., Svensson, PJ. et al. Nerve growth factor receptor immunohistochemistry has a limited additional value to diagnose Hirschsprung’s disease. Pediatr Surg Int 27, 431–435 (2011). https://doi.org/10.1007/s00383-010-2728-9

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  • DOI: https://doi.org/10.1007/s00383-010-2728-9

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