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Role of allopurinol on oxidative stress in caustic burn: cure for stricture?

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Abstract

An experimental study was conducted to investigate the effects of allopurinol, which inhibits the enzyme xanthine oxidase, on oxidative stress and on the prevention of stricture development after esophageal caustic injuries in rat. A randomized controlled study was conducted and 60 Wistar albino rats were divided into 6 equal groups, three groups for the acute phase and 3 groups for the chronic phase. Caustic esophageal burn was created by application of 37.5% NaOH to the distal esophagus. Allopurinol was administered at 40 mg/kg daily. Efficacy of the treatment for the acute phase was assessed by measuring tissue malondialdehyde (MDA), nitric oxide (NO) and glutathione (GSH) at the 3rd day; and for the chronic phase by determining tissue hydroxyproline content and histopathologic damage score at the 28th day. We found an increase in XO, MDA and GSH levels and a decrease in NO levels in the acute phase. Allopurinol reinstated the increase in XO significantly, while MDA, GSH and NO levels were reinstated insignificantly. There was no significant difference in means of tissue hydroxyproline content. Histopathologic damage scores were significantly lower in the allopurinol treated group. This study, which is to our knowledge, the first in the literature investigating the influence of allopurinol on caustic esophageal burn, reveals that allopurinol effects MDA, GSH and NO levels insignificantly in the acute phase of caustic esophageal burn and decreases fibrosis significantly in the chronic phase.

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Acknowledgments

The authors would like to thank Dr. Timur Kose, Ph.D from Ege University, School of Medicine, Department of Biostatistics, for the statistical analysis. We also thank technicians Mr. Ismail Zonguldak, Mr. Caglar Uz and Mr. Bulent Ata from Ege University, Department of Experimental Surgery, for technical assistance during the experimental procedures. The experiment protocol was approved by Ege University Experimental Ethics Committee and Ege University, Department of Experimental Surgery. The experiment comply with the current laws of the Turkish Republic.

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Authors and Affiliations

  1. Department of General Surgery, Ege University Hospital, Ege University, Bornova, Izmir, 35100, Turkey

    Ozer Makay & Sinan Ersin

  2. Department of Gastroenterology, Adnan Menderes University Hospital, Adnan Menderes University, Aydin, Turkey

    Vahit Yukselen

  3. Department of Pathology, SSK Izmir Training Hospital, Izmir, Turkey

    Enver Vardar

  4. Department of Biochemistry, Adnan Menderes University Hospital, Adnan Menderes University, Aydin, Turkey

    Cigdem Yenisey & Tuncay Bicakci

  5. Department of Gastroenterology, Ege University Hospital, Ege University, Bornova, Izmir, 35100, Turkey

    Omer Ozutemiz

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  1. Ozer Makay
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  2. Vahit Yukselen
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  3. Enver Vardar
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  4. Cigdem Yenisey
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  5. Tuncay Bicakci
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  6. Sinan Ersin
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  7. Omer Ozutemiz
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Correspondence to Ozer Makay.

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Makay, O., Yukselen, V., Vardar, E. et al. Role of allopurinol on oxidative stress in caustic burn: cure for stricture?. Pediatr Surg Int 23, 1105–1112 (2007). https://doi.org/10.1007/s00383-007-2009-4

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  • DOI: https://doi.org/10.1007/s00383-007-2009-4

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