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Extracellular Ca2+ uptake by T cells might help to make a diagnosis of acute rejection

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Abstract

To more non-invasively diagnose acute rejection, we focused on the uptake of extracellular Ca2+ by T cells as a result of the activation of Ca2+ release activated Ca2+ channels. A full thickness of the skin allograft model was established using BN rats as the donors and LEW rats as the recipients, and similar LEW rats as both donors and recipients in the control group. After transplantation, the grafts were staged histopathologically in both rats. The uptake of extaracellular 45Ca pre T cell was measured in the macrophage-treated and non-treated groups, and the ratios between the two groups were calculated and the results were compared according to the post-operative day. No histopathological findings of acute rejection were observed in the control group. The allograft model group showed acute rejection histopathologically beginning on day 2 and increased through day 5. The macrophage-treated model/non-treated model 45Ca uptake ratio (CAR) was significantly higher in the allograft rats on day 2. No significant difference was observed on day 4. Measuring the uptake of extracellular Ca2+ by recipient T cells using donor macrophages might be useful for making a diagnosis of acute rejection.

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References

  1. Crabtree GR (1989) Contingent genetic regulatory events in T lymphocyte activation. Science 243:355–361

    Article  PubMed  CAS  Google Scholar 

  2. Ullman KS, Northrop JP, Verweij CL, Crabtree GR (1990) Transmission of signals from the T lymphocyte antigen receptor to the genes responsible for cell proliferation and immune function: the missing link. Annu Rev Immunol 8:421–452

    Article  PubMed  CAS  Google Scholar 

  3. Donnadieu E, Bismuth G, Trautmann A (1992) Calcium fluxes in T lymphocytes. J Biol Chem 267:25864–25872

    PubMed  CAS  Google Scholar 

  4. Gardner JP, Balasubramanyam M (1996) Na–Ca exchange in circulating blood cells. Ann NY Acad Sci 779:502–514

    Article  PubMed  CAS  Google Scholar 

  5. Kotturi MF, Carlow DA, Lee JC, Ziltener HJ et al (2003) Identification and functional characterization of voltage-dependent calcium channels in T lymphocytes. J Biol Chem 278:46949–46960

    Article  PubMed  CAS  Google Scholar 

  6. Langhorst MF, Schwarzmann N, Guse AH (2004) Ca2+ release via ryanodine receptors and Ca2+ entry: major mechanisms in NAADP-mediated Ca2+ signaling in T-lymphocytes. Cell Signal 16:1283–1289

    Article  PubMed  CAS  Google Scholar 

  7. Fujita T, Takahashi S, Yagihashi A, Jimbow K et al (1997) Prolonged survival of rat skin allograft by treatment with FK506 ointment. Transplantation 64:922–925

    Article  PubMed  CAS  Google Scholar 

  8. Ohara K, Billington R, James RW, Dean GA et al (1990) Toxicologic evaluation of FK506. Transplant Proc 22:83

    PubMed  CAS  Google Scholar 

  9. Kumano K, Wang G, Endo T, Kuwano S (1991) FK506-induced nephrotoxicity in rats. Transplant Proc 23:512

    PubMed  CAS  Google Scholar 

  10. Calne RY, Lim S, Samaan A, Collier SJ et al (1989) Rapamycin for immunosuppression in organ allografting. Lancet 2:227

    Article  PubMed  CAS  Google Scholar 

  11. Whiting PH, Woo J, Adam BJ, Hasan NU et al (1991) Toxicity of rapamycin-A comparative and combination study with cyclosporine at immunotherapeutic dosage in the rat. Transplantation 52:203

    Article  PubMed  CAS  Google Scholar 

  12. Lewis RS (2001) Calcium signaling mechanisms in T lymphocytes. Annu Rev Immunol 19:497–521

    Article  PubMed  CAS  Google Scholar 

  13. Kunerth S, Mayr GW, Koch-nolte F, Guse AH (2003) Analysis of subcellular calcium signals in T-lymphocytes. Cell Signal 15:783–792

    PubMed  CAS  Google Scholar 

  14. Zweifach A, Lewis RS (1993) Mitogen-regulated Ca2+ current of T lymphocytes is activated by depletion of intracellular Ca2+ stores. Proc Natl Acad Sci 90:6295–6299

    Article  PubMed  CAS  Google Scholar 

  15. Fomina AF, Fanger CM, Kozak JA, Cahalan MD (2000) Single channel properties and regulated expression of Ca(2+) release-activated Ca(2+) (CRAC) channels in human T cells. J Cell Biol 150:1435–1444

    Article  PubMed  CAS  Google Scholar 

  16. Vennekens R, Voets T, Bindels RJ, Droogmans G et al (2002) Current understanding of mammalian TRP homologues. Cell Calcium 31:253–264

    Article  PubMed  CAS  Google Scholar 

  17. Yuan JP, Kiselyov K, Shin DM, Chen J et al (2003) Homer binds TRPC family channels and is required for gating of TRPC1 by IP3 receptors. Cell 114:777–789

    Article  PubMed  CAS  Google Scholar 

  18. Smani T, Zakharov SI, Leno E, Csutora P et al (2003) Ca2+-independent phospholipase A2 is novel determinant of store-operated Ca2+ entry. J Biol Chem 278:11909–11915

    Article  PubMed  CAS  Google Scholar 

  19. Liu J, Farmer JD Jr, Lane WS, Friedman J et al (1991) Calcineurin is a common target of cyclophilin-cyclosporin A and FKBP-FK506 complexes. Cell 66:807–815

    Article  PubMed  CAS  Google Scholar 

  20. Schreiber SL, Crabtree GR (1992) The mechanism of action of cyclosporine A and FK506. Immunol Today 13:136–142

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Pediatric Surgery, Kurume University School of Medicine, 67 Asahimachi, Kurume-City, Fukuoka, 830-0011, Japan

    Kenjiro Akiyoshi, Shigeki Hikida, Kimio Asagiri, Yoshiaki Tanaka & Minoru Yagi

  2. Radioisotope Institute for Basic and Clinical Medicine, Kurume University School of Medicine, Fukuoka, Japan

    Hiroyoshi Inoue

Authors
  1. Kenjiro Akiyoshi
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  2. Shigeki Hikida
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  3. Hiroyoshi Inoue
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  4. Kimio Asagiri
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  5. Yoshiaki Tanaka
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  6. Minoru Yagi
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Correspondence to Kenjiro Akiyoshi.

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Akiyoshi, K., Hikida, S., Inoue, H. et al. Extracellular Ca2+ uptake by T cells might help to make a diagnosis of acute rejection. Pediatr Surg Int 23, 149–153 (2007). https://doi.org/10.1007/s00383-006-1842-1

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  • DOI: https://doi.org/10.1007/s00383-006-1842-1

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