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Interleukin-6 changes tight junction permeability and intracellular phospholipid content in a human enterocyte cell culture model

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Abstract.

Proinflammatory cytokines and secretory phospholipase A2 (sPLA2) are elevated in patients with inflammatory bowel disease (IBD). We previously reported that the proinflammatory cytokine IL-6 increased the expression of sPLA2 (a hydrolyzer of phosphatidylcholine) and decreased membrane integrity in an intestinal epithelial cell culture model. To determine the physiological effects of the IL-6 mediated increase in sPLA2 on decreased epithelial layer integrity, we investigated alterations of intracellular/secretory phospholipid (PL) composition in a cell culture model. In addition, since other PLs may also mediate epithelial membrane activity, we investigated the effect of IL-6 on PL activity in a Caco-2 enterocyte culture model. Caco-2 cells were incubated for 72 h with IL-6 or media alone (control). Both media and cell lysate were analyzed for PL composition using thin-layer chromatography. The PL composition in the media did not show any differences between the two groups (p>0.1). Total intracellular PL contents were also unchanged; however, IL-6 led to significant changes in PL composition including an increase in phosphatidylethanolamine (PE) and sphingomyelin (SM) and a decrease in phosphatidylcholine (PC) and lysophosphatidylcholine (LPC) (p<0.05). Both PE and SM are known as inflammatory signaling factors involved in human IBD. Our study suggests that the decreased membrane integrity seen with IL-6 application may occur via intracellular PL alterations, rather than through the direct effects of sPLA2.

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Authors and Affiliations

  1. Section of Pediatric Surgery, University of Michigan Medical School, F3970 Mott Children's Hospital, Ann Arbor, Michigan, 48109-0245, USA

    Y. Tazuke, R. A. Drongowski, D. H. Teitelbaum & A. G. Coran

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  1. Y. Tazuke
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  2. R. A. Drongowski
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  3. D. H. Teitelbaum
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  4. A. G. Coran
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Correspondence to A. G. Coran.

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Tazuke, Y., Drongowski, R.A., Teitelbaum, D.H. et al. Interleukin-6 changes tight junction permeability and intracellular phospholipid content in a human enterocyte cell culture model. Ped Surgery Int 19, 321–325 (2003). https://doi.org/10.1007/s00383-003-1003-8

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  • DOI: https://doi.org/10.1007/s00383-003-1003-8

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