Child's Nervous System

, Volume 34, Issue 1, pp 79–84 | Cite as

Challenges for molecular and serological ZIKV infection confirmation

  • Zilton Farias Meira de Vasconcelos
  • Renata Campos Azevedo
  • Nathália Thompson
  • Leonardo Gomes
  • Letícia Guida
  • Maria Elisabeth Lopes Moreira
Focus Session

Abstract

Background

Zika Virus (ZIKV), member of Flaviviridae family and Flavivirus genus, has recently emerged as international public health emergency after its association with neonatal microcephaly cases. Clinical diagnosis hindrance involves symptom similarities produced by other arbovirus infections, therefore laboratory confirmation is of paramount importance.

Discussion

The most reliable test available is based on ZIKV RNA detection from body fluid samples. However, short viremia window periods and asymptomatic infections diminish the success rate for RT-PCR positivity. Beyond molecular detection, all serology tests in areas where other Flavivirus circulates proved to be a difficult task due to the broad range of cross-reactivity, especially with dengue pre-exposed individuals.

Conclusion

Altogether, lack of serological diagnostic tools brings limitations to any retrospective evaluation. Those studies are central in the context of congenital infection that could occur asymptomatically and mask prevalence and risk rates.

Keywords

ZIKV Arboviruses RT-PCR Serology PRNT Vertical transmission Microcephaly 

Notes

Acknowledgements

We thank Fernandes Figueira Institute Pediatric Infectious Disease Team for helpful discussions during this work and field expertise from ZIKVIRUSIFF Cohort study registered on clinicaltrials.gov with identifier NCT03255369.

Compliance with ethical standards

Conflict of interest

The authors declare no financial interests.

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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Zilton Farias Meira de Vasconcelos
    • 1
  • Renata Campos Azevedo
    • 2
  • Nathália Thompson
    • 1
  • Leonardo Gomes
    • 1
  • Letícia Guida
    • 1
  • Maria Elisabeth Lopes Moreira
    • 1
  1. 1.Fernandes Figueira Institute, FiocruzRio de JaneiroBrazil
  2. 2.Institute of Microbiology Paulo de Góes, Federal University of Rio de JaneiroRio de JaneiroBrazil

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