Abstract
Purpose
The goal of this study was to identify direct cerebrospinal fluid (CSF) pathways in the interface between ventricles and cisterns. Such routes are hypothesized to be involved in alternative CSF flows in abnormal circumstances of CSF circulation.
Methods
Chronic obstructive hydrocephalus models were induced in ten Sprague–Dawley rats with kaolin injection into the cisterna magna. Three weeks after the kaolin injection, when thick arachnoid fibrosis obliterated the fourth ventricular outlets, cationized ferritin was stereotactically infused as a tracer into the lateral ventricle in order to observe the pathways from the ventricles to the subarachnoid space. Animals were killed in 48 h and brains were sectioned. CSF flow pathways were traced by the staining of ferritin with ferrocyanide.
Results
Eight out of ten rats developed hydrocephalus. The subarachnoid membranes of the convexity and basal cisterns were severely adhered such that most of the ferritin remained in the ventricles whereas basal and convexity cisterns were clear of ferritin. In six out of the eight hydrocephalus rats, ferritin leaked from the third ventricle into the quadrigeminal cistern, and from the lateral ventricle into the ambient cistern.
Conclusions
The interfaces between the third ventricle and the quadrigeminal cistern, and between the lateral ventricle and the ambient cistern appear to be alternative CSF pathways in a pathologic condition such as obstructive hydrocephalus.
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References
Reiber H (2003) Proteins in cerebrospinal fluid and blood: barriers, CSF flow rate and source-related dynamics. Restor Neurol Neurosci 21:79–96
Nguyen T, Chin WC, O’Brien JA, Verdugo P, Berger AJ (2001) Intracellular pathways regulating ciliary beating of rat brain ependymal cells. J Physiol 531:131–140
Raichle ME, Grubb RL Jr (1978) Regulation of brain water permeability by centrally-released vasopressin. Brain Res 143:191–194
Rosenberg GA, Kyner WT, Fenstermacher JD, Patlak CS (1986) Effect of vasopressin on ependymal and capillary permeability to tritiated water in cat. Am J Physiol 251:F485–F489
Ghersi-Egea JF, Finnegan W, Chen JL, Fenstermacher JD (1996) Rapid distribution of intraventricularly administered sucrose into cerebrospinal fluid cisterns via subarachnoid velae in rat. Neuroscience 75:1271–1288
Penn RD, Lee MC, Linninger AA, Miesel K, Lu SN, Stylos L (2005) Pressure gradients in the brain in an experimental model of hydrocephalus. J Neurosurg 102:1069–1075
Shapiro K, Kohn IJ, Takei F, Zee C (1987) Progressive ventricular enlargement in cats in the absence of transmantle pressure gradients. J Neurosurg 67:88–92
Stephensen H, Tisell M, Wikkelso C (2002) There is no transmantle pressure gradient in communicating or noncommunicating hydrocephalus. Neurosurgery 50:763–771, discussion 771–763
Klarica M, Oreskovic D, Bozic B, Vukic M, Butkovic V, Bulat M (2009) New experimental model of acute aqueductal blockage in cats: effects on cerebrospinal fluid pressure and the size of brain ventricles. Neuroscience 158:1397–1405
Park JH, Park YS, Suk JS, Park SW, Hwang SN, Nam TK, Kim YB, Lee WB (2011) Cerebrospinal fluid pathways from cisterns to ventricles in N-butyl cyanoacrylate-induced hydrocephalic rats. J Neurosurg Pediatr 8:640–646
Park YS, Park SW, Suk JS, Nam TK (2011) Development of an acute obstructive hydrocephalus model in rats using N-butyl cyanoacrylate. Childs Nerv Syst 27:903–910
Parmley RT, Ostroy F, Gams RA, DeLucas L (1979) Ferrocyanide staining of transferrin and ferritin-conjugated antibody to transferrin. J Histochem Cytochem 27:681–685
Odake G, Yamaki T, Naruse S (1978) CSF-circulation pathways in experimental hydrocephalus of the rat (author’s transl). Neurol Med Chir (Tokyo) 18:673–680
Hochwald GM (1985) Animal models of hydrocephalus: recent developments. Proc Soc Exp Biol Med 178:1–11
Theil EC (1987) Ferritin: structure, gene regulation, and cellular function in animals, plants, and microorganisms. Annu Rev Biochem 56:289–315
Feldberg W (1976) The ventral surface of the brain stem: a scarcely explored region of pharmacological sensitivity. Neuroscience 1:427–441
Borison HL, Borison R, McCarthy LE (1980) Brain stem penetration by horseradish peroxidase from the cerebrospinal fluid spaces in the cat. Exp Neurol 69:271–289
Proescholdt MG, Hutto B, Brady LS, Herkenham M (2000) Studies of cerebrospinal fluid flow and penetration into brain following lateral ventricle and cisterna magna injections of the tracer [14C]inulin in rat. Neuroscience 95:577–592
McCormack EJ, Egnor MR, Wagshul ME (2007) Improved cerebrospinal fluid flow measurements using phase contrast balanced steady-state free precession. Magn Reson Imaging 25:172–182
Wagshul ME, Chen JJ, Egnor MR, McCormack EJ, Roche PE (2006) Amplitude and phase of cerebrospinal fluid pulsations: experimental studies and review of the literature. J Neurosurg 104:810–819
Greitz D (1993) Cerebrospinal fluid circulation and associated intracranial dynamics. A radiologic investigation using MR imaging and radionuclide cisternography. Acta Radiol Suppl 386:1–23
Gupta S, Soellinger M, Boesiger P, Poulikakos D, Kurtcuoglu V (2009) Three-dimensional computational modeling of subject-specific cerebrospinal fluid flow in the subarachnoid space. J Biomech Eng 131:021010
Howden L, Giddings D, Power H, Aroussi A, Vloeberghs M, Garnett M, Walker D (2008) Three-dimensional cerebrospinal fluid flow within the human ventricular system. Comput Methods Biomech Biomed Engin 11:123–133
Vladic A, Klarica M, Bulat M (2009) Dynamics of distribution of 3H-inulin between the cerebrospinal fluid compartments. Brain Res 1248:127–135
Bulat M, Lupret V, Orehkovic D, Klarica M (2008) Transventricular and transpial absorption of cerebrospinal fluid into cerebral microvessels. Coll Antropol 32(Suppl 1):43–50
Shapiro WR, Young DF, Mehta BM (1975) Methotrexate: distribution in cerebrospinal fluid after intravenous, ventricular and lumbar injections. N Engl J Med 293:161–166
Brightman MW (1965) The distribution within the brain of ferritin injected into cerebrospinal fluid compartments. I. Ependymal distribution. J Cell Biol 26:99–123
Barshes N, Demopoulos A, Engelhard HH (2005) Anatomy and physiology of the leptomeninges and CSF space. Cancer Treat Res 125:1–16
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Yoon, JS., Nam, Tk., Kwon, Jt. et al. CSF flow pathways through the ventricle–cistern interfaces in kaolin-induced hydrocephalus rats—laboratory investigation. Childs Nerv Syst 31, 2277–2281 (2015). https://doi.org/10.1007/s00381-015-2901-5
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DOI: https://doi.org/10.1007/s00381-015-2901-5