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Evaluation of VEGF-A in platelet and microRNA-126 in serum after coronary artery bypass grafting

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Abstract

Platelet functions are thought to contribute to clinical outcomes after heart surgery. This study was conducted to assess the pivotal roles of vascular endothelial growth factor-A (VEGF-A) and microRNA-126 (miR-126) during coronary artery bypass grafting (CABG). Whole blood was collected for platelet isolation from 67 patients who underwent CABG surgery between July 2013 and March 2014. VEGF-A and miR-126 levels in serum, plasma, and platelets were measured at various time points and compared with clinical characteristics. The platelet count was decreased at 3 days after CABG. This dynamic change in platelet count was larger after conventional coronary artery bypass (CCAB) than off-pump coronary artery bypass (OPCAB). VEGF-A in the same number of platelets (IP-VEGF-A) was increased at 3 days after CABG, followed by an increase of VEGF-A in serum (S-VEGF-A) at 7 days after surgery. The miR-126-3p level in serum (S-miR-126-3p) increased rapidly after CABG and then decreased below preoperative levels. The IP-VEGF-A level on day 7 after CABG in patients with peripheral artery disease (PAD), who suffered from endothelial dysfunction, was higher compared with patients without PAD. Conversely, S-miR-126-3p on day 7 after surgery was lower in patients with PAD than in patients without PAD. Low levels of S-miR-126-3p due to endothelial dysfunction may lead to high IP-VEGF-A, which is closely related to complications after CABG.

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Abbreviations

CABG:

Coronary artery bypass grafting

VEGF-A:

Vascular endothelial growth factor-A

IP-VEGF-A:

Intra-platelet vascular endothelial growth factor-A

S-VEGF-A:

Serum vascular endothelial growth factor-A

IP-miR-126:

Intra-platelet miR-126

IL-6:

Interleukin-6

TPO:

Thrombopoietin

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Acknowledgements

We thank Dr. Toshiaki Shimizu for useful discussions and Ms. Nobue Uto for technical assistance. We thank Mitchell Arico from Edanz Group (https://en-author-services.edanz.com/ac) for editing a draft of this manuscript.

Funding

This study was supported by Challenging Research (Exploratory) (18K19523) (MY), Grants-in-Aid for Scientific Research (18H02734) (MY), (16H05229) (TH), (19K09274) (YI), (20K09130) (HK), and (20K07852) (YO), and Grants-in Aid for Early career scientists (20K16553) (KT) and (20K16527) (TK).

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Author notes

  1. Teruto Hashiguchi and Yutaka Imoto these authors share senior authorship

Authors and Affiliations

  1. Department of Cardiovascular and Gastroenterological Surgery, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, 890-8520, Japan

    Kosuke Mukaihara, Hideaki Kanda, Yoshiya Shigehisa, Kenichi Arata, Kazuhisa Matsumoto & Yutaka Imoto

  2. Department of Laboratory and Vascular Medicine, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, 890-8520, Japan

    Munekazu Yamakuchi, Kazunori Takenouchi, Yoko Oyama, Toyoyasu Koriyama & Teruto Hashiguchi

Authors
  1. Kosuke Mukaihara
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  2. Munekazu Yamakuchi
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  10. Teruto Hashiguchi
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Contributions

KM, MY and HK designed and performed all the experiments, analyzed data, and wrote the manuscript. YS, KA, KM, KT, YO and TK partially performed the experiments. TH and YI supervised all the experiments. All authors discussed the design and the results.

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Correspondence to Munekazu Yamakuchi.

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Mukaihara, K., Yamakuchi, M., Kanda, H. et al. Evaluation of VEGF-A in platelet and microRNA-126 in serum after coronary artery bypass grafting. Heart Vessels 36, 1635–1645 (2021). https://doi.org/10.1007/s00380-021-01855-6

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  • DOI: https://doi.org/10.1007/s00380-021-01855-6

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