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Abnormal expression of long non-coding RNAs in myocardial infarction

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Abstract

Myocardial infarction (MI) is the leading cause of fatality worldwide. Our study aimed to investigate the dysregulated long non-coding RNA (lncRNA) in MI and elucidate the mechanism of it in MI. The lncRNA and mRNA expression profiling of the whole left ventricular tissue of MI mice model (8 mice) and Sham group (8 mice) was obtained based on microarray analysis. Differentially expressed lnRNAs/mRNA (DELs/DEMs) were identified in MI. DELs/DEMs co-expression network construction, gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis were conducted to predict the biological functions of DEMs. Quantitative real-time polymerase chain reaction (qRT-PCR) was subjected to validate the abnormally expressed DELs in left ventricular tissues of MI mice model. Total of 168 DELs (37 up- and 131 down-regulated) and 126 DEMs (87 up- and 39 down-regulated) were identified in MI compared with Sham group. The co-expression network of candidate DELs and DEMs was constructed, which covered 219 nodes and 1775 edges. The qRT-PCR validation results indicated that ENSMUST00000124047 was significantly down-regulated in MI group and AK166279 was significantly up-regulated in MI group. ENSMUST00000121611 and NR_015515 had the up-regulated tendency in MI group compared with Sham group. The DEMs in MI were significantly enriched in 41 signaling pathways including complement and coagulation cascades, cytokine–cytokine receptor interaction and chemokine signaling pathway. The expression profiling of dysregulated DELs in MI was identified. Our results might provide useful information for exploring the pathogenesis mechanism of MI.

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Authors and Affiliations

  1. Hangzhou JunKangYiDe Hospital, No.26 North Xueyuan Road, Hangzhou, 310011, Zhejiang Province, People’s Republic of China

    Tao Wu

  2. Department of Cardiology, The First Affiliated Hospital, School of Medicine, Zhejiang University, No. 79, Qingchun Road, ShangCheng District, Hangzhou, 310003, Zhejiang Province, People’s Republic of China

    Huan-dong Wu, Zao-xian Xu, Fei Han, Bi-qi Zhang, Jian Sun & Shen-jiang Hu

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Correspondence to Shen-jiang Hu.

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The work was supported by The National Natural Sciences Foundation of China (Project for Young Scientists, 30800999) and The National Natural Sciences Foundation of China (Project of International Cooperation and Exchanges, 812111369).

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380_2017_990_MOESM6_ESM.tif

Figure S1: GO terms and KEGG signaling pathways enrichment of DEMs in MI. (A): molecular function enrichment of GO terms from DEMs; (B) biological process enrichment of GO terms from DEMs; (C): cellular component enrichment of GO terms from DEMs; (D): KEGG signaling pathway enrichment from DEMs. (TIFF 743 kb)

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Wu, T., Wu, Hd., Xu, Zx. et al. Abnormal expression of long non-coding RNAs in myocardial infarction. Heart Vessels 32, 1253–1261 (2017). https://doi.org/10.1007/s00380-017-0990-7

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