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Sodium hydrosulfide alleviates pulmonary artery collagen remodeling in rats with high pulmonary blood flow

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Abstract

This study aimed to explore the effect of sodium hydrosulfide (NaHS) on pulmonary artery collagen remodeling in rats with high pulmonary blood flow. Thirty-two Sprague-Dawley rats were randomly divided into a sham group, shunt group, sham + NaHS (an H2S donor) group, and shunt + NaHS group. After 11 weeks of shunting, mean pulmonary artery pressure (MPAP), relative median area (RMA) of pulmonary arteries, H2S concentration in lung tissues, plasma endothelin-1 (ET-1) levels, and ET-1 mRNA in lung tissues were investigated. Collagen I and collagen III were evaluated by immunohistochemistry. Hydroxyproline assay and Sirius-red staining were performed. Matrix metalloproteinase-13 (MMP-13), tissue inhibitor of metalloproteinase-1 (TIMP-1), and connective tissue growth factor (CTGF) were evaluated by immunohistochemistry. After 11 weeks of shunting, rats showed a significant pulmonary hypertension and pulmonary artery collagen remodeling in association with a decrease in lung tissue H2S content. After NaHS treatment for 11 weeks, lung tissue H2S content was increased, whereas MPAP was attenuated and RMA was reduced. Meanwhile, pulmonary artery collagen I and collagen III protein expressions of intra-acinar pulmonary arteries were inhibited, but MMP-13/TIMP-1 ratio was augmented with a decreased plasma ET-1 content and lung tissue ET-1mRNA and CTGF expressions. The downregulation of H2S is involved in the development of pulmonary artery collagen remodeling induced by high pulmonary blood flow.

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Authors and Affiliations

  1. Department of Pediatrics, Peking University First Hospital and Key Laboratory of Molecular Cardiology, Ministry of Education, Xi-An Men Street No. 1, West District, Beijing, 100034, P.R. China

    Xiaohui Li, Junbao Du & Hongfang Jin

  2. Institute of Cardiovascular Research, Peking University First Hospital, Beijing, P.R. China

    Bin Geng & Chaoshu Tang

Authors
  1. Xiaohui Li
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  2. Junbao Du
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  3. Hongfang Jin
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  4. Bin Geng
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  5. Chaoshu Tang
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Correspondence to Junbao Du.

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Li, X., Du, J., Jin, H. et al. Sodium hydrosulfide alleviates pulmonary artery collagen remodeling in rats with high pulmonary blood flow. Heart Vessels 23, 409–419 (2008). https://doi.org/10.1007/s00380-008-1059-4

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  • DOI: https://doi.org/10.1007/s00380-008-1059-4

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