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Enhanced angiogenesis by gelatin hydrogels incorporating basic fibroblast growth factor in rabbit model of hind limb ischemia

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Abstract

Recently we have developed new sustained release system of basic fibroblast growth factor (bFGF) using gelatin hydrogel as a carrier. Using this system, we examined the effect of topical sustained release of bFGF on angiogenesis and tissue blood perfusion in a rabbit model of hind limb ischemia. Thirty-two rabbits underwent excision of right femoral artery under general anesthesia. Two weeks later the rabbits were randomized into four groups (n = 8 each): no treatment, intramuscular injection of gelatin hydrogel alone, and intramuscular injection of gelatin hydrogel incorporating 30 µg and 100 µg of bFGF. Four weeks after each treatment, selective angiography, tissue blood flowmetry using laser Doppler perfusion imaging, and histological examination of thigh muscle were performed. In groups treated with bFGF incorporating gelatin hydrogel, tissue blood flow, number of arterioles, and vascular density were significantly increased in a dose-dependent manner 4 weeks after the treatment. Serum concentrations of bFGF and vascular endothelial growth factor were not elevated 4 weeks after the treatment. In conclusion, sustained release of bFGF using gelatin hydrogel augmented angiogenesis and improved tissue blood flow after excision of the femoral artery.

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Authors and Affiliations

  1. Department of Cardiovascular Surgery, Kyoto University Graduate School of Medicine, 54 Kawaharacho, Shogoin, Sakyo-ku, Kyoto, 606-8507, Japan

    Kazuhiko Doi, Tadashi Ikeda, Akira Marui, Yoshio Arai, Keiichi Hirose, Yoshiharu Soga, Atsushi Iwakura, Koji Ueyama, Kazunobu Nishimura & Masashi Komeda

  2. Insititute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan

    Toshihiro Kushibiki & Yasuhiko Tabata

  3. Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, Kyoto, Japan

    Kenichi Yamahara & Hiroshi Itoh

Authors
  1. Kazuhiko Doi
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  2. Tadashi Ikeda
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  3. Akira Marui
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  4. Toshihiro Kushibiki
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  5. Yoshio Arai
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  6. Keiichi Hirose
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  7. Yoshiharu Soga
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  8. Atsushi Iwakura
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  9. Koji Ueyama
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  10. Kenichi Yamahara
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  11. Hiroshi Itoh
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  12. Kazunobu Nishimura
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  13. Yasuhiko Tabata
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  14. Masashi Komeda
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Corresponding author

Correspondence to Masashi Komeda.

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Doi, K., Ikeda, T., Marui, A. et al. Enhanced angiogenesis by gelatin hydrogels incorporating basic fibroblast growth factor in rabbit model of hind limb ischemia. Heart Vessels 22, 104–108 (2007). https://doi.org/10.1007/s00380-006-0934-0

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  • DOI: https://doi.org/10.1007/s00380-006-0934-0

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