3D skeleton reconstructions of natural earthworm burrow systems using CAT scan images of soil cores

Abstract

 Four soil cores (length, 20 cm; diameter, 16 cm) were sampled in a Swiss pre-Alpine meadow with high earthworm abundance (>400 individuals/m²); two cores were taken in October 1993 and the other two cores in April 1994. The cores were described using computer assisted tomography which gives a series of section images every 3 mm. A method for reconstructing the three-dimensional (3D) skeleton of the earthworm burrow system is presented and discussed. This method provides an image of the structural organisation of the burrow system and was found to be adequately sensitive for use in ecological and functional studies. The seasonal variation of these 3D skeletons was investigated using two approaches, i.e. the analysis of: (1) global burrow system characteristics, and (2) individual burrow characteristics. At the scale of the global burrow system no difference was found between seasons (same number of burrows and same total burrow length) except for the vertical segment distribution, which was homogeneous in spring and decreased with depth in the fall. The study of individual burrow characteristics revealed that burrows tended to be more vertical in spring and that their branching intensity was higher in this season.