Effect of low pO2 on colonization of maize roots by a genetically altered Pseudomonas putida [PH6(L1019)]

Abstract

Among the factors which may affect colonization of roots by soil bacteria is that of rhizosphere oxygen partial pressure (pO₂). The oxygen concentration in the root zone influences both microbes and roots. Roots exposed to low pO₂, as might occur during flooding and waterlogging of the soil, become more leaky and loss of soluble carbon increases. To determine whether periods of low pO₂ increased root colonization by a genetically altered pseudomonad we inoculated 3- to 4-week-old maize plants, grown in soil and transferred to a hydroponic system or grown in fritted clay, with Pseudomonas putida PH6(L1019)(lacZY+) following exposure of the roots to air or cylinder N₂. Numbers of heterotrophs and the marked pseudomonad were determined by dilution plating. Low pO₂ generally increased the numbers of bacteria associated with roots exposed to the treatments in solution or in undisturbed fritted clay rooting medium. Under low pO₂ in a hydroponic system, roots of intact maize plants tended also to have higher soluble organic C and hexose (anthrone-detectable sugars) than roots exposed to air. The effect of low pO₂ was most pronounced in the fritted clay where low pO₂ favored colonization by the marked strain; numbers were 3- to 96-fold greater than those on roots flushed with air but accounted for only 0.06–0.61% of the total population. Roots exposed to low pO₂ tended to accumulate more C. Results suggest that in the fritted clay, the pseudomonad was able to exploit the increased C supply and to achieve greater numbers on roots exposed to low pO₂, whereas the dilution of carbon released from roots in the hydroponic apparatus did not allow for the same magnitude of increase on roots.