Abstract
Rare bacteria in rhizospheres from natural watermelon vs chimeric watermelon were investigated to understand the rare rhizobacteria assembling processes along with plant development and effects of rare species on functional stability. Over 80% of the total OTUs were defined as rare taxa (i.e., transient, permanent, and conditionally rare) in the rhizosphere. Among these three rarities, transient rare taxa possessed the highest richness, while the conditionally rarity displayed the largest variations along growth stages and exhibited the greatest deterministic process for assembling. Pairs of conditionally rare taxa with high asynchrony and similar functional potentials were identified in the rhizobacterial communities. This suggests the functional stability of the rhizosphere through functional redundancy. The number of pairs within the rhizosphere of chimeric plant was ~ two fold higher than that of natural watermelon, which illustrated that chimeric plants can stabilize rhizospheric functions.
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The raw sequences of 16S rRNA gene amplicons were submitted to the NCBI Sequence Read Archive (http://www.ncbi.nlm.nih.gov/Traces/sra/) database under the accession number SRP313306.
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We thank the staff of the Nanjing Institute of Vegetable Science for providing seedlings.
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This study was supported by the National Nature Science Foundation of China (31972506; 31772398).
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NL conceived the ideas and designed the study; YR and TTW conducted the pot experiment and DNA extraction. YR analyzed the data and wrote the manuscript with substantial contributions from SWG, QWH, QRS, and NL. All authors gave final approval for publication.
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Ruan, Y., Wang, T., Guo, S. et al. Chimeric plants favor asynchrony of conditionally rare bacterial species facilitating functional complementarity in rhizosphere. Biol Fertil Soils 58, 459–470 (2022). https://doi.org/10.1007/s00374-022-01637-w
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DOI: https://doi.org/10.1007/s00374-022-01637-w