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Biochar co-application mitigated the stimulation of organic amendments on soil respiration by decreasing microbial activities in an infertile soil

Biology and Fertility of Soils volume 57, pages 793–807 (2021)Cite this article

Abstract

A field experiment was carried out to study the responses of soil respiration, soil C and N, composition of main microbial groups, enzyme activity, and microbial C source utilization to two organic amendments (i.e., straw and manure) added alone or combined with biochar for 9 months in an infertile soil. In comparison with the non-amended control, soil CO2 emission rates were significantly increased following straw and manure addition, but they were decreased by 19% and 36% on average when co-applied with biochar. Organic amendments significantly increased the size of labile C pool by 21%, total phospholipid-fatty acid (PLFA) concentrations by 32%, activities of α-glucosidase by 51%, β-glucosidase by 115%, β-D-cellobiosidase by 105%, β-xylosidase by 43%, phenol oxidase by 59%, and peroxidase by 91% on average relative to the non-amended control, whereas biochar co-application with organic amendments had no effect on soil PLFA concentrations but significantly decreased the enzyme activities by 38% and the sizes of labile C and N pools by 16% on average. The results of the substrate-induced respiration confirmed a lower capacity of biochar-treated soils to use the C sources of carbohydrates and carboxylic acids. Structural equation modeling indicated that the response of soil CO2 emission rates to organic amendment and biochar was directly linked to their effects on microbial activities. Therefore, our study demonstrated that biochar co-applied with straw or manure mitigated soil C loss by decreasing soil microbial activities.

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References

  • Agegnehu G, Srivastava AK, Bird MI (2017) The role of biochar and biochar-compost in improving soil quality and crop performance: a review. Appl Soil Ecol 119:156–170

    Google Scholar 

  • Ai C, Liang G, Sun J, Wang X, Zhou W (2012) Responses of extracellular enzyme activities and microbial community in both the rhizosphere and bulk soil to long-term fertilization practices in a fluvo-aquic soil. Geoderma 173–174:330–338

    Google Scholar 

  • Amelung W, Bossio D, de Vries W, Kögel-Knabner I, Lehmann J, Amundson R, Bol R, Collins C, Lal R, Leifeld J, Minasny B, Pan G, Paustian K, Rumpel C, Sanderman J, van Groenigen JW, Mooney S, van Wesemael B, Wander M, Chabbi A (2020) Towards a global-scale soil climate mitigation strategy. Nat Commun 11:5427

    CAS  PubMed  PubMed Central  Google Scholar 

  • Bailey VL, Fansler SJ, Smith JL, Bolton H Jr (2011) Reconciling apparent variability in effects of biochar amendment on soil enzyme activities by assay optimization. Soil Biol Biochem 43:296–301

    CAS  Google Scholar 

  • Belay-Tedla A, Zhou X, Su B, Wan S, Luo Y (2009) Labile, recalcitrant, and microbial carbon and nitrogen pools of a tallgrass prairie soil in the US Great Plains subjected to experimental warming and clipping. Soil Biol Biochem 41:110–116

    CAS  Google Scholar 

  • Bhattacharyya P, Roy KS, Neogi S, Adhya TK, Rao KS, Manna MC (2012) Effects of rice straw and nitrogen fertilization on greenhouse gas emissions and carbon storage in tropical flooded soil planted with rice. Soil till Res 124:119–130

    Google Scholar 

  • Blagodatskaya E, Kuzyakov Y (2008) Mechanisms of real and apparent priming effects and their dependence on soil microbial biomass and community structure: critical review. Biol Fertil Soils 45:115–131

    Google Scholar 

  • Bossio DA, Scow KM (1998) Impact of carbon and flooding on PLFA profiles and substrate utilization patterns of soil microbial communities. Microb Ecol 35:265–278

    CAS  PubMed  Google Scholar 

  • Brookes PC, Chen Y, Chen L, Qiu G, Luo Y, Xu J (2017) Is the rate of mineralization of soil organic carbon under microbiological control? Soil Biol Biochem 112:127–139

    CAS  Google Scholar 

  • Campbell CD, Chapman SJ, Cameron CM, Davidson MS, Potts JM (2003) A rapid microtiter plate method to measure carbon dioxide evolved from carbon substrate amendments so as to determine the physiological profiles of soil microbial communities by using whole soil. Appl Environ Microb 69:3593–3599

    CAS  Google Scholar 

  • Cao XY, Pignatello JJ, Li Y, Lattao C, Chappell MA, Chen N, Miller LF, Mao JD (2012) Characterization of wood chars produced at different temperatures using advanced solid-state 13C NMR spectroscopic techniques. Energ Fuel 26:5983–5991

    CAS  Google Scholar 

  • Chen J, Chen D, Xu Q, Fuhrmann JJ, Li L, Pan G, Li Y, Qin H, Liang C, Sun X (2019) Organic carbon quality, composition of main microbial groups, enzyme activities, and temperature sensitivity of soil respiration of an acid paddy soil treated with biochar. Biol Fertil Soils 55:185–197

    CAS  Google Scholar 

  • Chen J, Li S, Liang C, Xu Q, Li Y, Qin H, Fuhrmann JJ (2017) Response of microbial community structure and function to short-term biochar amendment in an intensively managed bamboo (Phyllostachys praecox) plantation soil: effect of particle size and addition rate. Sci Total Environ 574:24–33

    CAS  PubMed  Google Scholar 

  • Chen J, Liu X, Li L, Zheng J, Qu J, Zheng J, Zhang X, Pan G (2015) Consistent increase in abundance and diversity but variable change in community composition of bacteria in topsoil of rice paddy under short term biochar treatment across three sites from South China. Appl Soil Ecol 91:68–79

    CAS  Google Scholar 

  • Chen J, Sun X, Li L, Liu X, Zhang B, Zheng J, Pan G (2016) Change in active microbial community structure, abundance and carbon cycling in an acid rice paddy soil with the addition of biochar. Eur J Soil Sci 67:857–867

    CAS  Google Scholar 

  • Conant RT, Ryan MG, Ågren GI, Birge HE, Davidson EA, Eliasson PE, Evans SE, Frey SD, Giardina CP, Hopkins FM, Hyvönen R, Kirschbaum MUF, Lavallee JM, Leifeld J, Parton WJ, Megan Steinweg J, Wallenstein MD, Martin Wetterstedt JÅ, Bradford MA (2011) Temperature and soil organic matter decomposition rates – synthesis of current knowledge and a way forward. Global Change Biol 17:3392–3404

    Google Scholar 

  • Dai Z, Zhang X, Tang C, Muhammad N, Wu J, Brookes PC, Xu J (2017) Potential role of biochars in decreasing soil acidification – a critical review. Sci Total Environ 581:601–611

    PubMed  Google Scholar 

  • DeForest JL (2009) The influence of time, storage temperature, and substrate age on potential soil enzyme activity in acidic forest soils using MUB-linked substrates and l-DOPA. Soil Biol Biochem 41:1180–1186

    CAS  Google Scholar 

  • Dong H, Ge J, Sun K, Wang B, Xue J, Wakelin SA, Wu J, Sheng W, Liang C, Xu Q, Jiang P, Chen J, Qin H (2021) Change in root-associated fungal communities affects soil enzymatic activities during Pinus massoniana forest development in subtropical China. Forest Ecol Manag 482:118817

    Google Scholar 

  • Duan M, Wu F, Jia Z, Wang S, Cai Y, Chang SX (2020) Wheat straw and its biochar differently affect soil properties and field-based greenhouse gas emission in a Chernozemic soil. Biol Fertil Soils 56:1023–1036

    CAS  Google Scholar 

  • Elzobair KA, Stromberger ME, Ippolito JA, Lentz RD (2016) Contrasting effects of biochar versus manure on soil microbial communities and enzyme activities in an Aridisol. Chemosphere 142:145–152

    CAS  PubMed  Google Scholar 

  • Fanin N, Bertrand I (2016) Aboveground litter quality is a better predictor than belowground microbial communities when estimating carbon mineralization along a land-use gradient. Soil Biol Biochem 94:48–60

    CAS  Google Scholar 

  • Farrell M, Kuhn TK, Macdonald LM, Maddern TM, Murphy DV, Hall PA, Singh BP, Baumann K, Krull ES, Baldock JA (2013) Microbial utilisation of biochar-derived carbon. Sci Total Environ 465:288–297

    CAS  PubMed  Google Scholar 

  • Food and Agriculture Organisation of the United Nations (FAO) (2019) Recarbonization of Global Soils - a dynamic response to offset global emissions, FAO, http://www.fao.org/3/i7235en/I7235EN.pdf

  • Frostegård A, Bååth E (1996) The use of phospholipid fatty acid analysis to estimate bacterial and fungal biomass in soil. Biol Fertil Soils 22:59–65

    Google Scholar 

  • García-Orenes F, Roldán A, Morugán-Coronado A, Linares C, Cerdà A, Caravaca F (2016) Organic fertilization in traditional Mediterranean grapevine orchards mediates changes in soil microbial community structure and enhances soil fertility. Land Degrad Dev 27:1622–1628

    Google Scholar 

  • García-Ruiz R, Ochoa V, HinojosaMB CJA (2008) Suitability of enzyme activities for the monitoring of soil quality improvement in organic agricultural systems. Soil Biol Biochem 40:2137–2145

    Google Scholar 

  • German DP, Weintraub MN, Grandy AS, Lauber CL, Rinkes ZL, Allison SD (2011) Optimization of hydrolytic and oxidative enzyme methods for ecosystem studies. Soil Biol Biochem 43:1387–1397

    CAS  Google Scholar 

  • Guo K, Zhao Y, Liu Y, Chen J, Wu Q, Ruan Y, Li S, Shi J, Zhao L, Sun X, Liang C, Xu Q, Qin H (2020) Pyrolysis temperature of biochar affects ecoenzymatic stoichiometry and microbial nutrient-use efficiency in a bamboo forest soil. Geoderma 363:114162

    CAS  Google Scholar 

  • Hill BH, Elonen CM, Jicha TM, Kolka RK, Lehto LLP, Sebestyen SD, Seifert-Monson LR (2014) Ecoenzymatic stoichiometry and microbial processing of organic matter in northern bogs and fens reveals a common P-limitation between peatland types. Biogeochemistry 120:203–224

    CAS  Google Scholar 

  • Hooper D, Coughlan J, Mullen M (2008) Structural equation modelling: guidelines for determining model fit. Articles 2

  • Hu YL, Wu FP, Zeng DH, Chang SX (2014) Wheat straw and its biochar had contrasting effects on soil C and N cycling two growing seasons after addition to a Black Chernozemic soil planted to barley. Biol Fertil Soils 50:1291–1299

    CAS  Google Scholar 

  • Jiang X, Denef K, Stewart C, Cotrufo MF (2016) Controls and dynamics of biochar decomposition and soil microbial abundance, composition, and carbon use efficiency during long-term biochar-amended soil incubations. Biol Fertil Soils 52:1–14

    CAS  Google Scholar 

  • Jones DL, Murphy DV, Khalid M, Ahmad W, Edwards-Jones G, DeLuca TH (2011) Short-term biochar-induced increase in soil CO2 release is both biotically and abiotically mediated. Soil Biol Biochem 43:1723–1731

    CAS  Google Scholar 

  • Keith A, Singh B, Singh BP (2011) Interactive priming of biochar and labile organic matter mineralization in a smectite-rich soil. Environ Sci Technol 45:9611–9618

    CAS  PubMed  Google Scholar 

  • Lal R (2004) Soil carbon sequestration impacts on global climate change and food security. Science 304:1623–1627

    CAS  PubMed  Google Scholar 

  • Lammirato C, Miltner A, Kaestner M (2011) Effects of wood char and activated carbon on the hydrolysis of cellobiose by β-glucosidase from Aspergillus niger. Soil Biol Biochem 43:1936–1942

    CAS  Google Scholar 

  • Lehmann J, Rillig MC, Thies J, Masiello CA, Hockaday WC, Crowley D (2011) Biochar effects on soil biota – a review. Soil Biol Biochem 43:1812–1836

    CAS  Google Scholar 

  • Lehmann J (2007) A handful of carbon. Nature 447:143–144

    CAS  PubMed  Google Scholar 

  • Li Y, Li Y, Chang SX, Yang Y, Fu S, Jiang P, Luo Y, Yang M, Chen Z, Hu S, Zhao M, Liang X, Xu Q, Zhou G, Zhou J (2018) Biochar reduces soil heterotrophic respiration in a subtropical plantation through increasing soil organic carbon recalcitrancy and decreasing carbon-degrading microbial activity. Soil Biol Biochem 122:173–185

    CAS  Google Scholar 

  • Lin Y, Ye G, Kuzyakov Y, Liu D, Fan J, Ding W (2019) Long-term manure application increases soil organic matter and aggregation, and alters microbial community structure and keystone taxa. Soil Biol Biochem 134:187–196

    CAS  Google Scholar 

  • Liu C, Lu M, Cui J, Li B, Fang C (2014) Effects of straw carbon input on carbon dynamics in agricultural soils: a meta-analysis. Glob Chang Biol 20:1366–1381

    PubMed  Google Scholar 

  • Liu S, Zhang Y, Zong Y, Hu Z, Wu S, Zhou J, Jin Y, Zou J (2016) Response of soil carbon dioxide fluxes, soil organic carbon and microbial biomass carbon to biochar amendment: a meta-analysis. GCB Bioenergy 8:392–406

    CAS  Google Scholar 

  • Liu Y, Guo K, Zhao Y, Li S, Wu Q, Liang C, Sun X, Xu Q, Chen J, Qin H (2020) Change in composition and function of microbial communities in an acid bamboo (Phyllostachys praecox) plantation soil with the addition of three different biochars. Forest Ecol Manag 473:118336

    Google Scholar 

  • Liu YR, Delgado-Baquerizo M, Wang JT, Hu HW, Yang Z, He JZ (2018) New insights into the role of microbial community composition in driving soil respiration rates. Soil Biol Biochem 118:35–41

    CAS  Google Scholar 

  • Lu R (1999) Analytical methods for soils and agricultural chemistry. China Agricultural Science and Technology Press, Beijing

    Google Scholar 

  • Lu W, Ding W, Zhang J, Li Y, Luo J, Bolan N, Xie Z (2014) Biochar suppressed the decomposition of organic carbon in a cultivated sandy loam soil: a negative priming effect. Soil Biol Biochem 76:12–21

    CAS  Google Scholar 

  • Luo Y, Durenkamp M, De Nobili M, Lin Q, Brookes PC (2011) Short term soil priming effects and the mineralisation of biochar following its incorporation to soils of different pH. Soil Biol Biochem 43:2304–2314

    CAS  Google Scholar 

  • Luo Y, Zang H, Yu Z, Chen Z, Gunina A, Kuzyakov Y, Xu J, Zhang K, Brookes PC (2017) Priming effects in biochar enriched soils using a three-source-partitioning approach: 14C labelling and 13C natural abundance. Soil Biol Biochem 106:28–35

    CAS  Google Scholar 

  • Mackie KA, Marhan S, Ditterich F, Schmidt HP, Kandeler E (2015) The effects of biochar and compost amendments on copper immobilization and soil microorganisms in a temperate vineyard. Agr Ecosyst Environ 201:58–69

    CAS  Google Scholar 

  • Maisto G, De Marco A, De Nicola F, Arena C, Vitale L, Virzo De Santo A (2010) Suitability of two types of organic wastes for the growth of sclerophyllous shrubs on limestone debris: a mesocosm trial. Sci Total Environ 408:1508–1514

    CAS  PubMed  Google Scholar 

  • Mitchell PJ, Simpson AJ, Soong R, Simpson MJ (2015) Shifts in microbial community and water-extractable organic matter composition with biochar amendment in a temperate forest soil. Soil Biol Biochem 81:244–254

    CAS  Google Scholar 

  • Nannipieri P, Trasar-Cepeda C, Dick RP (2018) Soil enzyme activity: a brief history and biochemistry as a basis for appropriate interpretations and meta-analysis. Biol Fertil Soils 54:11–19

    CAS  Google Scholar 

  • Nannipieri P, Ascher J, Ceccherini MT, Landi L, Pietramellara G, Renella G (2003) Microbial diversity and soil functions. Eur J Soil Sci 54:655–670

    Google Scholar 

  • Nguyen TT, Marschner P (2016) Soil respiration, microbial biomass and nutrient availability in soil after repeated addition of low and high C/N plant residues. Biol Fertil Soils 52:165–176

    CAS  Google Scholar 

  • Pan G, Smith P, Pan W (2009) The role of soil organic matter in maintaining the productivity and yield stability of cereals in China. Agr Ecosyst Environ 129:344–348

    Google Scholar 

  • Paz-Ferreiro J, Fu S, Méndez A, Gascó G (2015) Biochar modifies the thermodynamic parameters of soil enzyme activity in a tropical soil. J Soil Sediment 15:578–583

    CAS  Google Scholar 

  • Peng X, Zhu QH, Xie ZB, Darboux F, Holden NM (2016) The impact of manure, straw and biochar amendments on aggregation and erosion in a hillslope Ultisol. CATENA 138:30–37

    CAS  Google Scholar 

  • Qayyum MF, Steffens D, Reisenauer HP, Schubert S (2014) Biochars influence differential distribution and chemical composition of soil organic matter. Plant Soil Environ 60:337–343

    CAS  Google Scholar 

  • Rovira P, Vallejo VR (2002) Labile and recalcitrant pools of carbon and nitrogen in organic matter decomposing at different depths in soil: an acid hydrolysis approach. Geoderma 107:109–141

    CAS  Google Scholar 

  • Rutigliano F, Romano M, Marzaioli R, Baglivo I, Baronti S, Miglietta F, Castaldi S (2014) Effect of biochar addition on soil microbial community in a wheat crop. Eur J Soil Biol 60:9–15

    CAS  Google Scholar 

  • Saiya-Cork KR, Sinsabaugh RL, Zak DR (2002) The effects of long term nitrogen deposition on extracellular enzyme activity in an Acer saccharum forest soil. Soil Biol Biochem 34:1309–1315

    CAS  Google Scholar 

  • Schmidt MWI, Torn MS, Abiven S, Dittmar T, Guggenberger G, Janssens IA, Kleber M, Kogel-Knabner I, Lehmann J, Manning DAC, Nannipieri P, Rasse DP, Weiner S, Trumbore SE (2011) Persistence of soil organic matter as an ecosystem property. Nature 478:49–56

    CAS  PubMed  Google Scholar 

  • Singh BP, Cowie AL (2014) Long-term influence of biochar on native organic carbon mineralisation in a low-carbon clayey soil. Sci Rep 4:3687

    PubMed  PubMed Central  Google Scholar 

  • Singh R, Singh P, Singh H, Raghubanshi AS (2019) Impact of sole and combined application of biochar, organic and chemical fertilizers on wheat crop yield and water productivity in a dry tropical agro-ecosystem. Biochar 1:229–235

    Google Scholar 

  • Sinsabaugh RL (2010) Phenol oxidase, peroxidase and organic matter dynamics of soil. Soil Biol Biochem 42:391–404

    CAS  Google Scholar 

  • Smith JL, Collins HP, Bailey VL (2010) The effect of young biochar on soil respiration. Soil Biol Biochem 42:2345–2347

    CAS  Google Scholar 

  • Sohi SP (2012) Carbon storage with benefits. Science 338:1034–1035

    CAS  PubMed  Google Scholar 

  • Soil Science Society of China (1999) Soil physical and chemical analysis. agricultural science and technology, Beijing, 146–226 (in Chinese)

  • Tian J, Wang J, Dippold M, Gao Y, Blagodatskaya E, Kuzyakov Y (2016) Biochar affects soil organic matter cycling and microbial functions but does not alter microbial community structure in a paddy soil. Sci Total Environ 556:89–97

    CAS  PubMed  Google Scholar 

  • Troy SM, Lawlor PG, O’Flynn CJ, Healy MG (2013) Impact of biochar addition to soil on greenhouse gas emissions following pig manure application. Soil Biol Biochem 60:173–181

    CAS  Google Scholar 

  • Ventorino V, De Marco A, Pepe O, Virzo De Santo A, Moschetti G (2013) Impact of innovative agricultural practices of carbon sequestration on soil microbial community. In: Piccolo A (ed) Carbon sequestration in agricultural soils. Springer, Berlin, pp 145–177

    Google Scholar 

  • Wang J, Xiong Z, Kuzyakov Y (2016) Biochar stability in soil: meta-analysis of decomposition and priming effects. GCB Bioenergy 8:512–523

    CAS  Google Scholar 

  • Wang WJ, Dalal RC, Moody PW, Smith CJ (2003) Relationships of soil respiration to microbial biomass, substrate availability and clay content. Soil Biol Biochem 35:273–284

    CAS  Google Scholar 

  • Wang X, Song D, Liang G, Zhang Q, Ai C, Zhou W (2015) Maize biochar addition rate influences soil enzyme activity and microbial community composition in a fluvo-aquic soil. Appl Soil Ecol 96:265–272

    Google Scholar 

  • Weng Z, Van Zwieten L, Singh BP, Tavakkoli E, Joseph S, Macdonald LM, Rose TJ, Rose MT, Kimber SWL, Morris S, Cozzolino D, Araujo JR, Archanjo BS, Cowie A (2017) Biochar built soil carbon over a decade by stabilizing rhizodeposits. Nat Clim Change 7:371

    CAS  Google Scholar 

  • World Reference Base for Soil Resources (WRB) (2006) A framework for international classification, correlation and communication. Food and Agriculture Organization of the United Nations, Rome

    Google Scholar 

  • Wu F, Jia Z, Wang S, Chang S, Startsev A (2013) Contrasting effects of wheat straw and its biochar on greenhouse gas emissions and enzyme activities in a Chernozemic soil. Biol Fertil Soils 49:555–565

    CAS  Google Scholar 

  • Yang X, Meng J, Lan Y, Chen W, Yang T, Yuan J, Liu S, Han J (2017) Effects of maize stover and its biochar on soil CO2 emissions and labile organic carbon fractions in Northeast China. Agr Ecosyst Environ 240:24–31

    CAS  Google Scholar 

  • Zak DR, Pregitzer KS, Curtis PS, Holmes WE (2000) Atmospheric CO2 and the composition and function of soil microbial communities. Ecol Appl 10:47–59

    Google Scholar 

  • Zelles L (1997) Phospholipid fatty acid profiles in selected members of soil microbial communities. Chemosphere 35:275–294

    CAS  PubMed  Google Scholar 

  • Zhang X, Fang Q, Zhang T, Ma W, Velthof GL, Hou Y, Oenema O, Zhang F (2020) Benefits and trade-offs of replacing synthetic fertilizers by animal manures in crop production in China: a meta-analysis. Global Change Biol 26:888–900

    Google Scholar 

  • Zhou H, Zhang D, Wang P, Liu X, Cheng K, Li L, Zheng J, Zhang X, Zheng J, Crowley D, van Zwieten L, Pan G (2017) Changes in microbial biomass and the metabolic quotient with biochar addition to agricultural soils: a meta-analysis. Agr Ecosyst Environ 239:80–89

    CAS  Google Scholar 

  • Zhu Z, Ge T, Luo Y, Liu S, Xu X, Tong C, Shibistova O, Guggenberger G, Wu J (2018) Microbial stoichiometric flexibility regulates rice straw mineralization and its priming effect in paddy soil. Soil Biol Biochem 121:67–76

    CAS  Google Scholar 

Download references

Acknowledgements

We thank Dr. Paolo Nannipieri, the Editor-in-Chief, and the anonymous reviewers for their very valuable comments in improving both the language and scientific quality of the manuscript.

Funding

This work was funded by the National Natural Science Foundation of China under grant numbers 41977083, the National College Students’ Innovation Training Program (No. 201910341020), the Fundamental Research Funds for the Provincial Universities of Zhejiang (2020YQ004), and the Zhejiang Provincial Natural Science Foundation of China under grant number LGF18C160001.

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  1. Qifeng Wu and Ruiyuan Lian have contributed equally to this work.

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  1. State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Lin’an, Hangzhou, 311300, China

    Qifeng Wu, Ruiyuan Lian, Meixia Bai, Jianping Bao, Yang Liu, Chenfei Liang, Hua Qin, Junhui Chen & Qiufang Xu

  2. Agricultural Technology Extension Centre, Lin’an Municipal Bureau of Agriculture, Lin’an, Hangzhou, 311300, China

    Qifeng Wu & Songhao Li

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Wu, Q., Lian, R., Bai, M. et al. Biochar co-application mitigated the stimulation of organic amendments on soil respiration by decreasing microbial activities in an infertile soil. Biol Fertil Soils 57, 793–807 (2021). https://doi.org/10.1007/s00374-021-01574-0

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Keywords

  • Straw
  • Manure
  • Soil CO2 emission
  • Enzyme activity
  • Main microbial groups