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Soil pre-fumigation could effectively improve the disease suppressiveness of biofertilizer to banana Fusarium wilt disease by reshaping the soil microbiome

Biology and Fertility of Soils volume 54, pages 793–806 (2018)Cite this article

Abstract

Two seasonal pot experiments were conducted to investigate the effect of biofertilizer application after mixture of lime and ammonium bicarbonate (LA) fumigation, on banana Fusarium wilt disease suppression and soil microbial community composition. Biofertilizer application after LA fumigation decreased 80% of disease incidence compared to control of biofertilizer application to non-fumigated soil. Biofertilizer application after fumigation clearly manipulated soil microbial community composition as revealed by non-metric multidimensional scaling and Venn diagram. LA fumigation significantly reduced the abundance of F. oxysporum while biofertilizer application after fumigation could further decrease it. Furthermore, indigenous microbes, e.g., Bacillus, Pseudomonas, and Mortierella, were associated with disease suppression. Biofertilizer application after fumigation significantly (p < 0.05) increased the soil pH and content of soil total C and available P and K, and this probably reshaped soil microbial community as revealed by redundancy analysis and variance partitioning analysis. The observed disease suppression due to biofertilizer application after soil fumigation can be attributed to the reduced abundance of F. oxysporum by general suppression resulting from manipulated soil properties and recovered soil microbiome.

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Acknowledgements

We thank the banana orchard owner Mr. Yusheng Li for providing access to the experimental field for soil collecting and greenhouse facilities.

Funding

This work was supported by the National Key Research and Development Program (2016YFE0101100 and 2017YFD0202101); the National Key Basic Research Program of China (2015CB150500); the National Natural Science Foundation of China (31601836, 31572212 and 31672239); the China Postdoctoral Science Foundation (2016M590469); the China Postdoctoral Science Foundation (2016M590469 and 2018T110509); the Science and Technology Planning Project of Guangdong Province, China (2016B020202006); the Natural Science Foundation of Jiangsu Province, China (BK20150059); the Hainan Provincial Natural Science Foundation of China (317040); the Priority Academic Program Development of the Jiangsu Higher Education Institutions (PAPD); the 111 project (B12009); and the China Scholarship Council (award to Rong Li for 1-year abroad study).

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Authors and Affiliations

  1. Jiangsu Provincial Key Lab for Organic Solid Waste Utilization, National Engineering Research Center for Organic-based Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing Agricultural University, Nanjing, 210095, China

    Zongzhuan Shen, Chao Xue, Yannan Ou, Rong Li & Qirong Shen

  2. College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, 210095, China

    Zongzhuan Shen, Chao Xue, Yannan Ou, Rong Li & Qirong Shen

  3. Faculty of Veterinary and Agricultural Sciences, University of Melbourne, Melbourne, Victoria, 3010, Australia

    Paul W. J. Taylor

  4. Hainan key Laboratory for Sustainable Utilization of Tropical Bio-resources, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, 570228, China

    Beibei Wang, Yan Zhao & Yunze Ruan

  5. Ecology and Biodiversity Group, Department of Biology, Institute of Environmental Biology, Utrecht University, Utrecht, Netherlands

    Rong Li

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  1. Zongzhuan Shen
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  2. Chao Xue
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Correspondence to Rong Li.

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Shen, Z., Xue, C., Taylor, P.W.J. et al. Soil pre-fumigation could effectively improve the disease suppressiveness of biofertilizer to banana Fusarium wilt disease by reshaping the soil microbiome. Biol Fertil Soils 54, 793–806 (2018). https://doi.org/10.1007/s00374-018-1303-8

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  • DOI: https://doi.org/10.1007/s00374-018-1303-8

Keywords

  • Banana Panama disease
  • Disease suppression
  • Microbial diversity
  • Soil fumigation
  • Biofertilizer