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Biology and Fertility of Soils

, Volume 54, Issue 7, pp 793–806 | Cite as

Soil pre-fumigation could effectively improve the disease suppressiveness of biofertilizer to banana Fusarium wilt disease by reshaping the soil microbiome

  • Zongzhuan Shen
  • Chao Xue
  • Paul W. J. Taylor
  • Yannan Ou
  • Beibei Wang
  • Yan Zhao
  • Yunze Ruan
  • Rong LiEmail author
  • Qirong Shen
Original Paper

Abstract

Two seasonal pot experiments were conducted to investigate the effect of biofertilizer application after mixture of lime and ammonium bicarbonate (LA) fumigation, on banana Fusarium wilt disease suppression and soil microbial community composition. Biofertilizer application after LA fumigation decreased 80% of disease incidence compared to control of biofertilizer application to non-fumigated soil. Biofertilizer application after fumigation clearly manipulated soil microbial community composition as revealed by non-metric multidimensional scaling and Venn diagram. LA fumigation significantly reduced the abundance of F. oxysporum while biofertilizer application after fumigation could further decrease it. Furthermore, indigenous microbes, e.g., Bacillus, Pseudomonas, and Mortierella, were associated with disease suppression. Biofertilizer application after fumigation significantly (p < 0.05) increased the soil pH and content of soil total C and available P and K, and this probably reshaped soil microbial community as revealed by redundancy analysis and variance partitioning analysis. The observed disease suppression due to biofertilizer application after soil fumigation can be attributed to the reduced abundance of F. oxysporum by general suppression resulting from manipulated soil properties and recovered soil microbiome.

Keywords

Banana Panama disease Disease suppression Microbial diversity Soil fumigation Biofertilizer 

Notes

Acknowledgements

We thank the banana orchard owner Mr. Yusheng Li for providing access to the experimental field for soil collecting and greenhouse facilities.

Funding

This work was supported by the National Key Research and Development Program (2016YFE0101100 and 2017YFD0202101); the National Key Basic Research Program of China (2015CB150500); the National Natural Science Foundation of China (31601836, 31572212 and 31672239); the China Postdoctoral Science Foundation (2016M590469); the China Postdoctoral Science Foundation (2016M590469 and 2018T110509); the Science and Technology Planning Project of Guangdong Province, China (2016B020202006); the Natural Science Foundation of Jiangsu Province, China (BK20150059); the Hainan Provincial Natural Science Foundation of China (317040); the Priority Academic Program Development of the Jiangsu Higher Education Institutions (PAPD); the 111 project (B12009); and the China Scholarship Council (award to Rong Li for 1-year abroad study).

Supplementary material

374_2018_1303_MOESM1_ESM.docx (805 kb)
ESM 1 (DOCX 804 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Jiangsu Provincial Key Lab for Organic Solid Waste Utilization, National Engineering Research Center for Organic-based Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource UtilizationNanjing Agricultural UniversityNanjingChina
  2. 2.College of Resources and Environmental SciencesNanjing Agricultural UniversityNanjingChina
  3. 3.Faculty of Veterinary and Agricultural SciencesUniversity of MelbourneMelbourneAustralia
  4. 4.Hainan key Laboratory for Sustainable Utilization of Tropical Bio-resources, Institute of Tropical Agriculture and ForestryHainan UniversityHaikouChina
  5. 5.Ecology and Biodiversity Group, Department of Biology, Institute of Environmental BiologyUtrecht UniversityUtrechtNetherlands

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