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Biology and Fertility of Soils

, Volume 53, Issue 7, pp 811–821 | Cite as

Comparison of lime- and biochar-mediated pH changes in nitrification and ammonia oxidizers in degraded acid soil

  • Nikola TeutscherovaEmail author
  • Eduardo VazquezEmail author
  • Alberto Masaguer
  • Mariela Navas
  • Kate M Scow
  • Radomir Schmidt
  • Marta Benito
Original Paper

Abstract

Ca-amendments are recommended for soil fertility enhancement in acid soils. Biochar (Bc) can be used as an alternative for the same purpose. Biochar additions have been reported to alter microbial communities in soils and biogeochemical processes including nitrogen (N) cycling. In a microcosm experiment, we investigated the interactive effects of soil pH, the type of soil amendment (lime or biochar), and the NH4 + supply on net N mineralization and nitrification in degraded acid soil and on the abundance of ammonia-oxidizing bacteria (AOB) and archaea (AOA). Soil was incubated under native pH and CaCO3 or biochar-manipulated pH to reach pH 6.2 and 6.8 in the presence or absence of added ammonium for 70 days. Our results showed that Bc had a longer-lasting effect on soil pH than CaCO3, suggesting that Bc could be a preferable liming agent. Increased pH stimulated microbial activity and led to increased N mineralization, which was higher when CaCO3 was applied. Although pH increase and NH4 +-N addition had no immediate effect on nitrification, they synergically enhanced nitrification at the end of the experiment. The amoA gene of AOA consistently outnumbered that of AOB, whereas only AOB amoA gene abundance number was significantly correlated with nitrification and their abundance followed similar trend as NO3 -N during the incubation. In acid soils where AOB could play a significant role in nitrification, biochar could result in more pronounced changes in N cycle than lime application which could be of especially high interest in intensively managed soils with high N inputs.

Keywords

Archaea Bacteria Liming Net nitrification qPCR 

Notes

Acknowledgements

Support for this work was provided by project AGRISOST-CM (S2013/ABI-2717) from the Comunidad de Madrid and cofunded by the ESIF. Dr. Benito acknowledges a grant (PR2015-00046) from the Ministry of Education, Culture and Sport (Spain). Eduardo Vázquez thanks the Ministerio de Economía y Competitividad (Spain) for his FPU fellowship.

Supplementary material

374_2017_1222_MOESM1_ESM.pdf (124 kb)
ESM 1 (PDF 124 kb)
374_2017_1222_MOESM2_ESM.pdf (131 kb)
ESM 2 (PDF 131 kb)

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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  1. 1.Department of Crop Sciences and Agroforestry, Faculty of Tropical AgriSciencesCzech University of Life Sciences PragueSuchdolCzech Republic
  2. 2.Departamento de Producción Agraria, Escuela Técnica Superior Ingeniería Agronómica, Alimentaria y de BiosistemasUniversidad Politécnica de MadridMadridSpain
  3. 3.Department of Land, Air and Water ResourcesUniversity of CaliforniaDavisUSA

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