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Impact of land use and nutrient addition on phosphatase activities and their relationships with organic phosphorus turnover in semi-arid grassland soils

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Abstract

Information on the relationships between phosphatase activities and organic phosphorus (P) turnover is fundamental to understanding soil P dynamics but remains poorly understood. An 8-year field study was conducted in a steppe and an abandoned cropland under semi-arid grasslands to explore the effects of nitrogen (N) and P additions on P composition in soil as determined by 31P nuclear magnetic resonance (NMR) and associated phosphatase activities. Results showed that the phosphate monoester content, soil acid phosphomonoesterase, alkaline phosphomonoesterase, and phosphodiesterase activities were higher in the steppe than in the abandoned cropland soil. Nitrogen addition significantly suppressed phosphatase activities. Phosphorus addition significantly increased acid phosphomonoesterase, alkaline phosphomonoesterase, and phosphodiesterase activities in the steppe but significantly decreased them in the abandoned cropland. Structural equation modeling revealed that both phosphodiesterase and alkaline phosphomonoesterase activities showed significant negative effects on diesters and monoesters in the steppe, but there were no significant effects of phosphatase activities on organic P composition in the abandoned cropland. Our findings highlight the variation of dominant mechanisms involved in organic P turnover with land use change. Phosphorus deficiency in the steppe appeared to promote the production of phosphatases and the subsequent biochemical mineralization of organic P. While in the abandoned cropland, previous cultivation resulted in a proportionally greater loss of soil organic carbon than that of organic P, indicating that organic P was mineralized as a result of biological mineralization of organic matter.

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References

  • Aciego Pietri JC, Brookes PC (2008) Relationships between soil pH and microbial properties in a UK arable soil. Soil Biol Biochem 40:1856–1861

    Article  CAS  Google Scholar 

  • Acosta-Martínez V, Tabatabai MA (2011) Phosphorus cycle enzymes. In: Dick RP (ed) Methods of soil enzymology. Soil Science Society of America, Madison, pp 161–184

    Google Scholar 

  • Allison SD, Vitousek PM (2005) Responses of extracellular enzymes to simple and complex nutrient inputs. Soil Biol Biochem 37:937–944

    Article  CAS  Google Scholar 

  • Allison SD, Weintraub MN, Gartner TB, Waldrop MP (2011) Evolutionary-economic principles as regulators of soil enzyme production and ecosystem function. In: Shukla G, Varma A (eds) Soil enzymology. Soil biology 22. Springer, Berlin, pp 229–243

    Google Scholar 

  • An SS, Cheng Y, Huang YM, Liu D (2013) Effects of revegetation on soil microbial biomass, enzyme activities, and nutrient cycling on the loess plateau in China. Restor Ecol 21:600–607

    Article  Google Scholar 

  • Balota EL, Yada IF, Amaral H, Nakatani AS, Dick RP, Coyne MS (2014) Long-term land use influences soil microbial biomass P and S, phosphatase and arylsulfatase activities, and S mineralization in a Brazilian oxisol. Land Degrad Dev 25:397–406

    Article  Google Scholar 

  • Bunemann EK, Smernik R, Marschner P, McNeill A (2008) Microbial synthesis of organic and condensed forms of phosphorus in acid and calcareous soils. Soil Biol Biochem 40:932–946

    Article  Google Scholar 

  • Cade-Menun BJ, Preston CM (1996) A comparison of soil extraction procedures for 31P NMR spectroscopy. Soil Sci 161:770–785

    Article  CAS  Google Scholar 

  • Condron LM, Tiessen H (2005) Interactions of organic phosphorus in terrestrial ecosystems. In: Turner BL, Frossard E, Baldwin DS (eds) Organic phosphorus in the environment. CABI, Oxford, pp 295–307

    Chapter  Google Scholar 

  • Condron LM, Turner BL, Cade-Menun BJ (2005) Chemistry and dynamics of soil organic phosphorus. In: Sims JT, Sharpley AN (eds) Phosphorus: agriculture and the environment. Soil Science Society of America, Madison, pp 87–121

    Google Scholar 

  • Evrendilek F, Celik I, Kilic S (2004) Changes in soil organic carbon and other physical soil properties along adjacent Mediterranean forest, grassland, and cropland ecosystems in Turkey. J Arid Environ 59:743–752

    Article  Google Scholar 

  • FAO/ISRIC/ISSS (1998) World reference base for soil resources. FAO, Rome

    Google Scholar 

  • Fierer N, Jackson RB (2006) The diversity and biogeography of soil bacterial communities. Proc Natl Acad Sci U S A 103:626–631

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Galloway JN, Townsend AR, Erisman JW, Bekunda M, Cai Z, Freney JR, Martinelli LA, Seitzinger SP, Sutton MA (2008) Transformation of the nitrogen cycle: recent trends, questions, and potential solutions. Science 320:889–892

    Article  CAS  PubMed  Google Scholar 

  • Harrison AF (1983) Relationship between intensity of phosphatase activity and physico-chemical properties in woodland soils. Soil Biol Biochem 15:93–99

    Article  CAS  Google Scholar 

  • Harrison AF (1987) Soil organic phosphorus: a review of world literature. CAB International, Wallingford

    Google Scholar 

  • Johnson D, Leake JR, Lee JA (1999) The effects of quantity and duration of simulated pollutant nitrogen deposition on root-surface phosphatase activities in calcareous and acid grasslands: a bioassay approach. New Phytol 141:433–442

    Article  CAS  Google Scholar 

  • Killam K (1994) Soil ecology. Cambridge Press, New York, pp 24–28

    Google Scholar 

  • Kuo S (1996) Phosphorus. In: Sparks DL (ed) Methods of soil analysis. Part 3: chemical methods. Soil Science Society of America, Madison, pp 869–919

    Google Scholar 

  • Lindsay WL, Frazier AW, Stephenson HF (1962) Identification of reaction products from phosphate fertilizers in soils1. Soil Sci Soc Am J 26:446–452

    Article  CAS  Google Scholar 

  • Liu Q-Y, Tong Y-P (2003) The effects of land use change on the eco-environmental evolution of farming-pastoral region in northern China: with an emphasis on Duolun County in Inner Mongolia. Acta Ecol Sin 23:1025–1030

    Google Scholar 

  • Lovell RD, Jarvis SC, Bardgett RD (1995) Soil microbial biomass and activity in long-term grassland: effects of management changes. Soil Biol Biochem 27:969–975

    Article  CAS  Google Scholar 

  • McGill WB, Cole CV (1981) Comparative aspects of cycling of organic C, N, S and P through soil organic matter. Geoderma 26:267–286

    Article  CAS  Google Scholar 

  • Murphy J, Riley JP (1962) A modified single solution method for the determination of phosphate in natural waters. Anal Chim Acta 27:31–36

    Article  CAS  Google Scholar 

  • Nannipieri P, Johnson RL, Paul EA (1978) Criteria for measurement of microbial growth and activity in soil. Soil Biol Biochem 10:223–229

    Article  CAS  Google Scholar 

  • Nannipieri P, Giagnoni L, Landi L, Renella G (2011) Role of phosphatase enzymes in soil. In: Bünemann E, Oberson A, Frossard E (eds) Phosphorus in action. Soil biology 26. Springer Verlag, Berlin, pp 215–241

    Chapter  Google Scholar 

  • Nannipieri P, Giagnoni L, Renella G, Puglisi E, Ceccanti B, Masciandaro G, Fornasier F, Moscatelli MC, Marinari S (2012) Soil enzymology: classical and molecular approaches. Biol Fertil Soils 48:743–762

    Article  Google Scholar 

  • Neal JL (1973) Influence of selected grasses and forbs on soil phosphatase activity. Can J Soil Sci 53:119–121

    Article  CAS  Google Scholar 

  • Nelson DW, Sommers LE (1982) Total carbon, organic carbon, and organic matter. In: Page AL (ed) Methods of soil analysis. Part 2. Chemical and microbiological properties. American Society of Agronomy, Soil Science Society of America, Madison, pp 539–579

    Google Scholar 

  • Olsen SR, Cole CV, Watanabe FS, Dean LA (1954) Estimation of available phosphorus in soils by extraction with sodium bicarbonate. USDA Circular no 939, Washington

    Google Scholar 

  • Quiquampoix H, Mousain D (2005) Enzymatic hydrolysis of organic phosphorus. In: Turner BL, Frossard E, Baldwin DS (eds) Organic phosphorus in the environment. CAB International, Wallingford, pp 89–112

    Chapter  Google Scholar 

  • Renella G, Landi L, Ascher J, Ceccherini MT, Pietramellara G, Nannipieri P (2006) Phosphomonoesterase production and persistence and composition of bacterial communities during plant material decomposition in soils with different pH values. Soil Biol Biochem 38:795–802

    Article  CAS  Google Scholar 

  • Renella G, Landi L, Valori F, Nannipieri P (2007) Microbial and hydrolase activity after release of low molecular weight organic compounds by a model root surface in a clayey and a sandy soil. Appl Soil Ecol 36:124–129

    Article  Google Scholar 

  • Sinsabaugh RL, Reynolds H, Long TM (2000) Rapid assay for amidohydrolase (urease) activity in environmental samples. Soil Biol Biochem 32:2095–2097

    Article  CAS  Google Scholar 

  • Tarafdar JC, Jungk A (1987) Phosphatase activity in the rhizosphere and its relation to the depletion of soil organic phosphorus. Biol Fertil Soils 3:199–204

    Article  CAS  Google Scholar 

  • Tiessen H, Salcedo IH, Sampaio EVSB (1992) Nutrient and soil organic matter dynamics under shifting cultivation in semi-arid northeastern Brazil. Agr Ecosyst Environ 38:139–151

    Article  CAS  Google Scholar 

  • Turner BL, Haygarth PM (2005) Phosphatase activity in temperate pasture soils: potential regulation of labile organic phosphorus turnover by phosphodiesterase activity. Sci Total Environ 344:27–36

    Article  CAS  PubMed  Google Scholar 

  • Turner BL, Papházy MJ, Haygarth PM, McKelvie ID (2002) Inositol phosphates in the environment. Phil Trans R Soc Lond B 357:449–469

    Article  CAS  Google Scholar 

  • Turner BL, Chudek JA, Whitton BA, Baxter R (2003a) Phosphorus composition of upland soils polluted by long-term atmospheric nitrogen deposition. Biogeochemistry 65:259–274

    Article  CAS  Google Scholar 

  • Turner BL, Mahieu N, Condron LM (2003b) Phosphorus-31 nuclear magnetic resonance spectral assignments of phosphorus compounds in soil NaOH–EDTA extracts. Soil Sci Soc Am J 67:497–510

    Article  CAS  Google Scholar 

  • Turner BL, Mahieub N, Condron LM (2003c) The phosphorus composition of temperate pasture soils determined by NaOH–EDTA extraction and solution 31P NMR spectroscopy. Org Geochem 34:1199–1210

    Article  CAS  Google Scholar 

  • Turner BL, Condron LM, Richardson SJ, Peltzer DA, Allison VJ (2007) Soil organic phosphorus transformations during pedogenesis. Ecosystems 10:1166–1181

    Article  CAS  Google Scholar 

  • Wang R, Dorodnikov M, Yang S, Zhang Y, Filley TR, Turco RF, Zhang Y, Xu Z, Li H, Jiang Y (2015) Responses of enzymatic activities within soil aggregates to 9-year nitrogen and water addition in a semi-arid grassland. Soil Biol Biochem 81:159–167

    Article  CAS  Google Scholar 

  • Wei K, Chen Z, Zhu A, Zhang J, Chen L (2014a) Application of 31P NMR spectroscopy in determining phosphatase activities and P composition in soil aggregates influenced by tillage and residue management practices. Soil Till Res 138:35–43

    Article  Google Scholar 

  • Wei K, Chen ZH, Zhang XP, Liang WJ, Chen LJ (2014b) Tillage effects on phosphorus composition and phosphatase activities in soil aggregates. Geoderma 217–218:37–44

    Article  Google Scholar 

  • Xu Z, Wan S, Zhu G, Ren H, Han X (2010) The influence of historical land use and water availability on grassland restoration. Restor Ecol 18:217–225

    Article  CAS  Google Scholar 

  • Xu Z, Wan S, Ren H, Han X, Li MH, Cheng W, Jiang Y (2012) Effects of water and nitrogen addition on species turnover in temperate grasslands in northern China. PLoS One 7, e39762

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Xu Z, Ren H, Cai J, Wang R, He P, Li MH, Lewis BJ, Han X, Jiang Y (2015) Antithetical effects of nitrogen and water availability on community similarity of semiarid grasslands: evidence from a nine-year manipulation experiment. Plant Soil 397:357–369

    Article  CAS  Google Scholar 

  • Yadav RS, Tarafdar JC (2001) Influence of organic and inorganic phosphorus supply on the maximum secretion of acid phosphatase by plants. Biol Fertil Soils 34:140–143

    Article  CAS  Google Scholar 

  • Zhang TQ, MacKenzie AF (1997) Changes of soil phosphorous fractions under long-term corn monoculture. Soil Sci Soc Am J 61:485–493

    Article  CAS  Google Scholar 

  • Zhang AM, Chen ZH, Zhang GN, Chen LJ, Wu ZJ (2012) Soil phosphorus composition determined by 31P NMR spectroscopy and relative phosphatase activities influenced by land use. Eur J Soil Biol 52:73–77

    Article  Google Scholar 

Download references

Acknowledgments

This study was financially supported by the National Natural Science Foundation of China (grant numbers 41171241, 41201290, 41371251, and 31370009), the National Key Technology R&D Program of China (grant number 2012BAD14B04), and the Strategic Priority Research Program of the Chinese Academy of Sciences (grant number XDB15010403). We also thank Professor Jie Zhuang for the insightful comments on this manuscript.

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Authors and Affiliations

  1. Institute of Applied Ecology, Chinese Academy of Sciences, P. O. Box 417, Shenyang, 110016, China

    Jihui Tian, Kai Wei, Zhenhua Chen, Zhuwen Xu & Lijun Chen

  2. University of Chinese Academy of Sciences, Beijing, 100049, China

    Jihui Tian

  3. Faculty of Agriculture and Life Sciences, Lincoln University, P. O. Box 85084, 7647, Christchurch, New Zealand

    Leo M. Condron

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  1. Jihui Tian
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  2. Kai Wei
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Correspondence to Lijun Chen.

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Tian, J., Wei, K., Condron, L.M. et al. Impact of land use and nutrient addition on phosphatase activities and their relationships with organic phosphorus turnover in semi-arid grassland soils. Biol Fertil Soils 52, 675–683 (2016). https://doi.org/10.1007/s00374-016-1110-z

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