Biology and Fertility of Soils

, Volume 52, Issue 3, pp 341–351 | Cite as

Bacillus amyloliquefaciens T-5 may prevent Ralstonia solanacearum infection through competitive exclusion

  • Shiyong Tan
  • Yian Gu
  • Chunlan Yang
  • Yue Dong
  • Xinlan Mei
  • Qirong Shen
  • Yangchun Xu
Original Paper


Investigation of the properties and mechanisms of the interactions of root-colonizing biocontrol bacteria and plant pathogens is necessary to optimize the biocontrol strategies. In the present study, the interaction of a biocontrol strain Bacillus amyloliquefaciens T-5 tagged with a green fluorescent protein marker and a bacterial wilt pathogen Ralstonia solanacearum QL-Rs1115 tagged with red fluorescent protein marker was studied on tomato roots using different inoculation methods. The results showed that in the co-culture experiment, the population of pathogen QL-RFP was decreased by increasing the initial inoculum concentration of biocontrol strain. In the greenhouse experiment, both strains T-5-GFP and QL-RFP colonized tomato roots (root tips, root hairs, primary roots, and root junctions) and formed a biofilm on the root surfaces as determined by dilution plating and confocal laser scanning microscopy (CLSM) techniques. However, the root colonization of pathogen strain QL-RFP was almost completely suppressed in the presence of biocontrol strain T-5-GFP when both soil and plant seedlings were treated with T-5-GFP. The results of this study revealed the effectiveness of strain B. amyloliquefaciens T-5 as a biocontrol agent against tomato wilt pathogen and the significance of inoculation method used to inoculate biocontrol strain.


Biological control CLSM Fluorescent protein Root colonization Tomato 



This research was financially supported by the National Natural Science Foundation of China (41301262), the Innovative Research Team Development Plan of the Ministry of Education of China (IRT1256), the Priority Academic Program Development (PAPD) of Jiangsu Higher Education Institutions, and the 111 project (B12009). We would like to acknowledge Prof. Paolo Nannipieri from University of Florence, Italy, for his careful revisions of the manuscript.

Supplementary material

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Shiyong Tan
    • 1
    • 2
    • 3
    • 4
  • Yian Gu
    • 1
    • 3
  • Chunlan Yang
    • 1
    • 3
  • Yue Dong
    • 1
    • 3
  • Xinlan Mei
    • 1
    • 3
  • Qirong Shen
    • 1
    • 3
  • Yangchun Xu
    • 1
    • 3
  1. 1.National Engineering Research Center for Organic-based FertilizersNanjing Agricultural UniversityNanjingChina
  2. 2.Key Laboratory of Plant Nutrition and Biological Fertilizer, Ministry of AgricultureChangshaPeople’s Republic of China
  3. 3.Jiangsu Collaborative Innovation Center for Solid Organic Waste UtilizationNanjing Agricultural UniversityNanjingChina
  4. 4.Hunan Taigu Biotechnology Co., LtdChangshaChina

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