The responses of nitrification on intensively managed agricultural soils following long-term biochar (BC) amendment are poorly understood. The nitrification potential, abundance, and composition of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) in acidic oxisols and alkaline cambosols following a 3-year BC treatment were investigated using 42-day aerobic incubation, quantitative polymerase chain reaction (qPCR), and clone library approach, respectively. Fresh soils were collected from a wheat/millet rotated pot trial in which 0 (control), 2.25, and 22.5 Mg ha−1 rice straw BCs were added for six consecutive crop seasons. The 22.5 Mg ha−1 BC (BC22.5) treatment enhanced nitrification in oxisols and even altered nitrification pattern from zero-order to first-order reaction model. AOA and AOB gene copies in the BC22.5 treatment were 9.55 and 22.0 times, respectively, compared with those in the BC0 treatment. The relative abundance of operational taxonomic units (OTUs) in AOA group 1.1a changed due to BC application, and that of OTU-20 was high in group 1.1b-related under the BC22.5 treatment. AOB community composition shifted toward Nitrosospira cluster 3 and 3-related group under the BC22.5 treatment. Basal nitrification was already high in cambosols, and BC had minimal effect on nitrification or AOA/AOB abundance. However, the BC22.5 treatment increased the relative abundance of OTU-9 in Nitrosospira cluster 3 group and that of OTU-13 and OTU-16 in Nitrosospira cluster 3-related groups both being AOB. The BC amendment had minimal effect on ammonia oxidizer composition in cambosols but influenced ammonia oxidizer composition and stimulated nitrification activity in oxisols.
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Ball PN, Mackenzie MD, Deluca TH, Holben WE (2010) Wildfire and charcoal enhance nitrification and ammonia-oxidizing bacterial abundance in dry Montane forest soils. J Environ Qual 39:1243–1253
Berglund LM, DeLuca TH, Zackrisson O (2004) Activated carbon amendments to soil alters nitrification rates in Scots pine forests. Soil Biol Biochem 36:2067–2073
Cai ZC, Qin SW (2006) Dynamics of crop yields and soil organic carbon in a long-term fertilization experiment in the Huang-Huai-Hai plain of China. Geoderma 136:708–715
Case SDC, McNamara NP, Reay DS, Stott AW, Grant HK, Whitaker J (2015) Biochar suppresses N2O emissions while maintaining N availability in a sandy loam soil. Biol Fertil Soils 81:178–185
Cheng CH, Lehmann J, Thies JE, Burton SD (2008) Stability of black carbon in soils across a climatic gradient. J Geophys Res 113:G02027
Clough TJ, Condron LM (2010) Biochar and the nitrogen cycle: introduction. J Environ Qual 39:1218–1223
De Boer W, Kowalchuk GA (2001) Nitrification in acid soils: microorganisms and mechanisms. Soil Biol Biochem 33:853–866
DeLuca TH, Nilsson MC, Zackrisson O (2002) Nitrogen mineralization and phenol accumulation along a fire chronosequence in northern Sweden. Oecologia 133:206–214
DeLuca TH, MacKenzie MD, Gundale MJ, Holben WE (2006) Wildfire-produced charcoal directly influences nitrogen cycling in ponderosa pine forests. Soil Sci Soc Am J 70:448–453
Ducey TF, Ippolito JA, Cantrell KB, Novak JM, Lentz RD (2013) Addition of activated switchgrass biochar to an aridic subsoil increases microbial nitrogen cycling gene N abundances. Appl Soil Ecol 65:65–72
Erguder TH, Boon N, Wittebolle L, Marzorati M, Verstraete W (2009) Environmental factors shaping the ecological niches of ammonia-oxidizing archaea. FEMS Microbiol Ecol 33:855–869
FAO (Food and Agricultural Organization of the United Nations) (2001) Soil carbon sequestration for improved land management. World Soil Resources Reports 96. Rome. Italy
Francis CA, Roberts KJ, Beman JM, Santoro AE, Oakley BB (2005) Ubiquity and diversity of ammonia-oxidizing archaea in water columns and sediments of the ocean. Proc Natl Acad Sci U S A 102:14683–14688
Gao Y, Chen WZ, Zhan HL, He LJ (2013) The analysis of influencing factors on grain potential yield growth (in Chinese). Chin Agric Sci Bull 29:132–138
Grossman JM, O’Neill BE, Tsai SM, Liang BQ, Neves E, Lehmann J, Thies JE (2010) Amazonian anthrosols support similar microbial communities that differ distinctly from those extant in adjacent, unmodified soils of the same mineralogy. Microb Ecol 60:192–205
Gubry-Rangin C, Nicol GW, Prosser JI (2010) Archaea rather than bacteria control nitrification in two agricultural acidic soils. FEMS Microbiol Ecol 74:566–574
Gubry-Rangin C, Hai B, Quince C, Engel M, Thomson BC, James P, Schloter M, Griffiths RI, Prosser JI, Nicol GW (2011) Niche specialization of terrestrial archaeal ammonia oxidizers. Proc Natl Acad Sci U S A 108:21206–21211
Guo JH, Liu XJ, Zhang Y, Shen JL, Han WX, Zhang WF, Christie P, Goulding KWT, Vitousek PK, Zhang FS (2010) Significant acidification in major Chinese croplands. Science 327:1008–1010
Hu L, Cao L, Zhang R (2014) Bacterial and fungal taxon changes in soil microbial community composition induced by short-term biochar amendment in red oxidized loam soil. World J Microbiol Biotechnol 30:1085–1092
Jia ZJ, Conrad R (2009) Bacteria rather than Archaea dominate microbial ammonia oxidation in an agricultural soil. Environ Microbiol 11:1658–1671
Joseph SD, Camps-Arbestain M, Lin Y, Munroe P, Chia CH, Hook J, Van Zwieten L, Kimber S, Cowie A, Singh BP, Lehmann J, Foidl N, Smernik RJ, Amonette JE (2010) An investigation into the reactions of biochar in soil. Aust J Soil Res 48:501–515
Ju XT, Xing GX, Chen XP, Zhang SL, Zhang LJ, Cui ZL, Yin B, Christie P, Zhu ZL, Zhang FS (2009) Reducing environmental risk by improving N management in intensive Chinese agricultural systems. Proc Natl Acad Sci U S A 106:8077–8078
Khodadad CLM, Zimmerman AR, Green SJ, Uthandi S, Foster JS (2011) Taxa-specific changes in soil microbial community composition induced by pyrogenic carbon amendments. Soil Biol Biochem 43:385–392
Kim JS, Sparovek G, Longo RM, De Melo WJ, Crowley D (2007) Bacterial diversity of terra preta and pristine forest soil from the Western Amazon. Soil Biol Biochem 39:684–690
Kowalchuk GA, Stephen JR, De Boer W, Prosser JI, Embley TM, Woldendorp JW (1997) Analysis of ammonia-oxidizing bacteria of the beta subdivision of the class Proteobacteria in coastal sand dunes by denaturing gradient gel electrophoresis and sequencing of PCR amplified 16S ribosomal DNA fragments. Appl Environ Microbiol 63:1489–1497
Lai L, Huang XJ, Wang H (2009) Estimation of environmental costs of chemical fertilizer utilization in China (in Chinese). Acta Pedol Sin 46:63–69
Lal R (2004) Soil carbon sequestration impacts on global climate change and food security. Science 304:1623–1627
Lehmann J, Rillig MC, Thies J, Masiello CA, Hockaday WC, Crowley D (2011) Biochar effects on soil biota—a review. Soil Biol Biochem 43:1812–1836
Lehtovirta LE, Prosser JI, Nicol GW (2009) Soil pH regulates the abundance and diversity of Group 1.1c Crenarchaeota. FEMS Microbiol Ecol 70:367–376
MacKenzie MD, DeLuca TH, Sala A (2006) Fire exclusion and nitrogen mineralization in low elevation forests of western Montana. Soil Biol Biochem 38:952–961
Mary B, Recous S, Robin D (1998) A model for calculating nitrogen fluxes in soil using 15N tracing. Soil Biol Biochem 30:1963–1979
McLaren AD (1970) Temporal and vectorial reactions of nitrogen in soil: a review. Can J Soil Sci 50:97–109
Nelissen V, Rütting T, Huygens D, Staelens J, Ruysschaert G, Boeckx P (2012) Maize biochars accelerate short-term soil nitrogen dynamics in a loam sand soil. Soil Biol Biochem 55:20–27
Noyce GL, Basiliko N, Fulthorpe R, Sackett TE, Thomas SC (2015) Soil microbial responses over 2 years following biochar addition to a north temperate forest. Biol Fertil Soils 1-11
Pereira EIP, Suddick EC, Mansour I, Mukome FND, Parikh SJ, Scow K, Johan S (2015) Biochar alters nitrogen transformations but has minimal effects on nitrous oxide emissions in an organically managed lettuce mesocosm. Biol Fertil Soils 51:1–10
Prommer J, Wanek W, Hofhansl F, Trojan D, Offre P, Urich T, Schleper C, Sassmann S, Kitzler B, Soja G, Hood-Nowotny RC (2014) Biochar decelerates soil organic nitrogen cycling but stimulates soil nitrification in a temperate arable field trial. PLoS One 9:e86388
Rotthauwe JH, Witzel KP, Liesack W (1997) The ammonia monooxygenase structural gene amoA as a functional marker: molecular fine-scale analysis of natural ammonia-oxidizing populations. Appl Environ Microbiol 63:4704–4712
Schloss PD, Larget BR, Handelsman J (2004) Integration of microbial ecology and statistics: a test to compare gene libraries. Appl Environ Microbiol 70:5485–5492
Shen JP, Zhang LM, Di HJ, He JZ (2012) A review of ammonia-oxidizing bacteria and archaea in Chinese soils. Front Microbiol 3:296
Sohi SP, Krull E, Lopez-Capel E, Bol R (2010) A review of biochar and its use and function in soil. Adv Agron 105:47–82
Song YJ, Zhang XL, Ma B, Chang SX, Gong J (2014) Biochar addition affected the dynamics of ammonia oxidizers and nitrification in microcosms of a coastal alkaline soil. Biol Fertil Soils 50:321–332
Steinbeiss S, Gleixner G, Antonietti M (2009) Effect of biochar amendment on soil carbon balance and soil microbial activity. Soil Biol Biochem 41:1301–1310
Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739
Wang SQ, Zhao X, Xing GX, Yang LZ (2012) Large-scale biochar production from crop residue: a new idea and the biogas-energy pyrolysis system. Bioresources 8:8–11
Wells GF, Park HD, Yeung CH, Eggleston B, Francis CA, Criddle CS (2009) Ammonia-oxidizing communities in a highly aerated full-scale activated sludge bio-reactor: betaproteobacterial dynamics and low relative abundance of Crenarchaea. Environ Microbiol 11:2310–2328
Zhao W, Cai ZC, Xu ZH (2007) Does ammonium-based N addition influence nitrification and acidification in humid subtropical soils of China? Plant Soil 297:213–221
Zhao X, Wang SQ, Xing GX (2013) Nitrification, acidification, and nitrogen leaching from subtropical cropland soils as affected by rice straw-based biochar: laboratory incubation and column leaching studies. J Soils Sediments 14:471–482
Zhao X, Wang JW, Hu HJ, Zhou CJ, Wang SQ, Xing GX (2014) Effects of crop-straw biochar on crop growth and soil fertility over a wheat/millet rotation in soils of China. Soil Use Manag 30:311–319
We would like to express our deep gratitude to the Editor-in-Chief, Paolo Nannipieri, for his help in English expressions and writing. This study was financially supported by the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB15040202), the National Natural Science Foundation of China (Grants 41271312 and 41001147), and the young foundation of State key Laboratory of Soil and Sustainable Agriculture, Chinese Academy of Sciences (Y412010006). Sincere thanks go to the valuable suggestions from anonymous reviewers. We also specially express thanks to Prof. Zhongjun Jia, Dr. Baozhan Wang, and Dr. Jun Zhao in Institute of Soil Science, Chinese Academy of Sciences, for their technical supports on analysis of ammonia oxidizers.
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He, L., Liu, Y., Zhao, J. et al. Comparison of straw-biochar-mediated changes in nitrification and ammonia oxidizers in agricultural oxisols and cambosols. Biol Fertil Soils 52, 137–149 (2016). https://doi.org/10.1007/s00374-015-1059-3
- Nitrification activity
- Nitrification kinetics
- Soil property