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Diverse bacterial communities are recruited on spores of different arbuscular mycorrhizal fungal isolates

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Abstract

Arbuscular mycorrhizal fungi (AMF) establish mutualistic symbioses with the roots of most food crops, playing a key role in soil fertility and plant nutrition and health. The beneficial activity of AMF may be positively affected by bacterial communities living associated with mycorrhizal roots, spores and extraradical hyphae. Here, we investigated the diversity of bacterial communities associated with the spores of six AMF isolates, belonging to different genera and species and maintained for several generations in pot cultures with the same host plant, under the same environmental conditions and with the same soil. The occurrence of large bacterial communities intimately associated with spores of the AMF isolates was revealed by PCR denaturing gradient gel electrophoresis (DGGE) analysis and sequencing of DGGE bands. Cluster and canonical correspondence analysis showed that the six AMF isolates displayed diverse bacterial community profiles unrelated with their taxonomic position, suggesting that each AMF isolate recruits on its spores a different microbiota. The 48 sequenced fragments were affiliated with Actinomycetales, Bacillales, Pseudomonadales, Burkholderiales, Rhizobiales and with Mollicutes-related endobacteria (Mre). For the first time, we report the occurrence of Mre in Funneliformis coronatum and Rhizophagus intraradices and sequences related to endobacteria of Mortierella elongata in F. coronatum and Funneliformis mosseae. The bacterial species identified are known to possess diverse and specific physiological characteristics and may play multifunctional roles affecting the differential performance of AMF isolates, in terms of infectivity and efficiency.

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Acknowledgments

The authors thank Dr. Cristiana Sbrana for providing the AMF isolates and Dr. Luciano Avio for providing the AMF isolates and for help in statistical analyses. This work was financially supported by a University of Pisa grant (Fondi di Ateneo).

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Authors and Affiliations

  1. Department of Agriculture, Food and Environment, University of Pisa, Via del Borghetto 80, 56124, Pisa, Italy

    Monica Agnolucci, Fabio Battini, Caterina Cristani & Manuela Giovannetti

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  1. Monica Agnolucci
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  2. Fabio Battini
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  4. Manuela Giovannetti
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Correspondence to Monica Agnolucci.

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Agnolucci, M., Battini, F., Cristani, C. et al. Diverse bacterial communities are recruited on spores of different arbuscular mycorrhizal fungal isolates. Biol Fertil Soils 51, 379–389 (2015). https://doi.org/10.1007/s00374-014-0989-5

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