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Biology and Fertility of Soils

, Volume 50, Issue 4, pp 583–592 | Cite as

A novel phosphorus biofertilization strategy using cattle manure treated with phytase–nanoclay complexes

  • Daniel Menezes-Blackburn
  • Milko A. Jorquera
  • Liliana Gianfreda
  • Ralf Greiner
  • María de la Luz Mora
Original Paper

Abstract

The aim of this work was to evaluate the treatment of cattle manure with phytases stabilized in allophanic nanoclays as a potential novel phosphorus (P) biofertilization technology for crops grown in volcanic soils (Andisol). Furthermore, because the optimal pH for commercial phytase catalysis does not match the natural pH of manure, a complementary experiment was set up to evaluate the effect of manure inoculation with an alkaline phytase-producing bacterium. Finally, phytase-treated soil, manure, and soil–manure mixtures were evaluated for their P-supplying capacity to wheat plants grown under greenhouse conditions. Treating cattle manure with phytases stabilized in nanoclays resulted in a significant (P ≤ 0.05) increase of inorganic P in soil extracts (NaOH-EDTA and Olsen). The use of phytase-treated cattle manure increased dry weights by 10 % and the P concentration by 39 % in wheat plants grown under greenhouse conditions, which is equivalent to a P fertilizer rate of about 150 kg of P per hectare. The inoculation of cattle manure with β-propeller phytase-producing bacteria led to an ∼10 % increase in inorganic P in the manure extracts. However, applying inoculated manure to soil did not significantly increase wheat yield or P acquisition responses. Our results suggest that the novel approach of incubating cattle manure with phytases stabilized in nanoclay enhances the organic P cycling and P nutrition of plants grown in P-deficient soils.

Keywords

Phytase Phytate Phosphorus Organic phosphorus Biofertilization Volcanic soils Cattle manure Wheat 

Notes

Acknowledgments

This work was financed by FONDECYT research projects no 1100625 and no 1120505. D. Menezes-Blackburn acknowledges to the following financial supports: UFRO and CONICYT Doctoral Scholarships, and Georg Forster Research Postdoctoral Fellowship (Humboldt Foundation). MA Jorquera also thanks to the support from International Cooperation Research CONICYT-BMBF code 2009-183.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Daniel Menezes-Blackburn
    • 1
    • 4
  • Milko A. Jorquera
    • 2
  • Liliana Gianfreda
    • 3
  • Ralf Greiner
    • 4
  • María de la Luz Mora
    • 2
  1. 1.Chile–Italy International Doctorate in Environmental Resources ScienceUniversidad de La FronteraTemucoChile
  2. 2.Scientific and Technological Bioresource Nucleus (BIOREN)Universidad de La FronteraTemucoChile
  3. 3.Dipartimento di Scienze del Suolo, della Pianta e dell’Ambiente, e delle Produzioni AnimaliUniversità di NapoliPorticiItaly
  4. 4.Department of Food Technology and Bioprocess Engineering, Max Rubner-InstitutFederal Research Institute of Nutrition and FoodKarlsruheGermany

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