Skip to main content

Diversity and stress tolerance in rhizobia from Parque Chaqueño region of Argentina nodulating Prosopis alba

Abstract

The aim of this work was to investigate the genetic diversity, symbiotic effectiveness, drought tolerance, and indole acetic acid production of indigenous rhizobial populations in the Parque Chaqueño of Argentina able to nodulate Prosopis alba, the dominant forest tree of this region. The populations were sampled at five locations from the Arid, Semi-arid, and Humid Chaco in the Parque Chaqueño region. A set of rhizobial strains able to nodulate P. alba was obtained and selected based on their molecular diversity. Data obtained by BOX-PCR indicated that the highest molecular variability was observed in rhizobial isolates from Semi-arid Chaco. High level of indolic compound production and tolerance to osmotic treatment were significantly (p ≤ 0.05) correlated with water restrictions of the environments where the strains belonged. A small set of rhizobial strains that stimulate P. alba growth was selected from a large group of strains. The strains were identified by 16S rDNA sequencing as belonging to the genera Mesorhizobium, Bradyrhizobium, and Ensifer. To our knowledge, this is the first report of P. alba nodulation by strains other than Mesorhizobium chacoense, which was already described for the Parque Chaqueño.

This is a preview of subscription content, access via your institution.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

References

  • Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (1987) Current protocols in molecular biology. Greene Publishing Associates/Wiley Interscience, New York

    Google Scholar 

  • Baca BE, Elmerich C (2007) Microbial production of plants hormones by microorganisms. In: Elmerich C, Newton W (eds) Associative nitrogen-fixation Bacteria and Cyanobacteria. IV Series Nitrogen Fixation: Origins, Applications, and Research Progress. Springer, Dordrecht, pp 113–137

    Chapter  Google Scholar 

  • Balzarini MG, Di Rienzo JA (2011) InfoGen version 2011. FCA, Universidad Nacional de Córdoba, Argentina. URL http://www.info-gen.com.ar

  • Balzarini MG, Bruno C, Fernandez E (2011) Multivariate analysis in phytopathology: options and opportunities in data mining to face new molecular information. In: Rodriguez Herrera R, Aguilar C, Simpson-Williamson J, Gutierrez Sanchez G (eds) Phytopathology in the omics era. Research Signpost, Kerala, pp 1–20

    Google Scholar 

  • Broughton WJ, Dilworth MJ (1971) Control of leghemoglobin synthesis in snake beans. Biochem J 125:1075–1080

    PubMed  CAS  Google Scholar 

  • Burkart A (1976) A monograph of the genus Prosopis (Leguminosae subfam. Mimosoideae): catalogue of the recognized species of Prosopis. J Arnold Arbor 57:450–525

    Google Scholar 

  • Cesco S, Mimmo T, Tonon G, Tomasi N, Pinton R, Terzano R, Neumann G, Weisskopf L, Renella G, Landi L, Nannipieri P (2012) Plant-borne flavonoids released into the rhizosphere: impact on soil bio-activities related to plant nutrition. A review. Biol Fertil Soils 48:123–149

    Article  CAS  Google Scholar 

  • Chang WS, Franck WL, Cytryn E, Jeong S, Joshi T, Emerich DW, Sadowsky MJ, Xu D, Stacey G (2007) An oligonucleotide microarray resource for transcriptional profiling of Bradyrhizobium japonicum. MPMI 20:1298–1307

    PubMed  Article  CAS  Google Scholar 

  • Di Rienzo JA, Casanoves F, Balzarini MG, Gonzalez L, Tablada M, Robledo CW (2012) InfoStat versión 2012. Grupo InfoStat, FCA, Universidad Nacional de Córdoba, Argentina. URL http://www.infostat.com.ar

  • Gel Compar Applied Maths NV

  • Glickman E, Dessaux Y (1995) A critical examination of the specificity of the Salkowski reagent for indolic compounds produced by phytopathogenic bacteria. Appl Environ Microbiol 61:793–796

    Google Scholar 

  • Haukka K, Lindstro K, Young P (1998) Three phylogenetic groups of nodA and nifH genes in Sinorhizobium and Mesorhizobium isolates from leguminous trees growing in Africa and Latin America. Appl Environ Microbiol 64:419–426

    PubMed  CAS  Google Scholar 

  • Iglesias O, Rivas R, Garcia-Fraile P, Abril A, Mateos P, Martinez-Molina E, Velázquez E (2007) Genetic characterization of fast-growing rhizobia able to nodulate Prosopis alba in North Spain. FEMS Microbiol Lett 277:210–216

    PubMed  Article  CAS  Google Scholar 

  • Laranjo M, Oliveira S (2011) Tolerance of Mesorhizobium type strains to different environmental stresses. Antonie van Leeuwenhoek 99:651–662

    PubMed  Article  CAS  Google Scholar 

  • Melchiorre M, de Luca M, Gonzalez Anta G, Suarez P, Lopez C, Lascano R, Racca R (2011) Bradyrhizobia strains isolation from field-grown soybean plants in Argentina and evaluation of their performance as improved inoculants. Biol Fertil Soils 47:81–89

    Article  Google Scholar 

  • Mottura M (2006) Development of microsatellites in Prosopis spp and their application to study the reproduction system. PhD thesis. Georg-August University of Göttingen, Germany

  • Räsänen LR, Sprent JI, Lindstrom K (2001) Symbiotic properties of Sinorhizobia isolated from Acacia and Prosopis nodules in Sudan and Senegal. Plant Soil 235:193–210

    Article  Google Scholar 

  • Shannon CE, Weaver W (1949) The mathematical theory of communication. University of Illinois Press, Urbana, pp 3–24

    Google Scholar 

  • Spaepen S, Vanderleyden J, Remans R (2007) Indole-3-acetic acid in microbial and microorganism–plant signaling. FEMS Microbiol Rev 31:425–448

    PubMed  Article  CAS  Google Scholar 

  • Sprent J, James EK (2007) Legume evolution: where do nodules and mycorrhizas fit in? Plant Physiol 144:575–581

    PubMed  Article  CAS  Google Scholar 

  • Steenhoudt O, Vanderleyden J (2000) Azospirillum, a free-living nitrogen-fixing bacterium closely associated with grasses: genetic, biochemical and ecological aspects. FEMS Microbiol Rev 24:487–506

    PubMed  Article  CAS  Google Scholar 

  • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance and maximum parsimony methods. Mol Biol Evol 28:2731–2739

    PubMed  Article  CAS  Google Scholar 

  • van der Weele CM, Spollen WG, Sharp RE, Baskin TI (2000) Growth of Arabidopsis thaliana seedlings under water deficit studied by control of water potential in nutrient–agar media. J Exp Bot 51:1555–1562

    PubMed  Article  Google Scholar 

  • Velázquez E, Igual JM, Willems A, Fernandez MP, Munoz E, Mateos PF, Abril A, Toro N, Normand P, Cervantes E, Gillis M, Martinez-Molina E (2001) Mesorhizobium chacoense sp. nov., a novel species that nodulates Prosopis alba in the Chaco Arido region (Argentina). Int J Syst Evol Microbiol 51:1011–1021

    PubMed  Article  Google Scholar 

  • Versalovic J, Schneider M, de Bruijn F, Lupski JR (1994) Genomic fingerprinting of bacteria using repetitive sequence-based polymerase chain reaction. Methods Mol Cell Biol 5:25–40

    CAS  Google Scholar 

  • Verzino G, Carranza C, Ledesma M, Joseau J, Di Rienzo J (2003) Adaptive genetic variation of Prosopis chilensis (Mol) Stuntz. Preliminary results from one test-site. For Ecol Manage 175:119–129

    Article  Google Scholar 

  • Vincent JM (1970) The cultivation, isolation and maintenance of rhizobia. In: Vincent JM (ed) A manual for the practical study of root-nodule bacteria. Blackwell Scientific, Oxford, pp 1–13

    Google Scholar 

  • Zahran H (2001) Rhizobia from wild legumes: diversity, taxonomy, ecology, nitrogen fixation and biotechnology. J Biotechnol 91:143–153

    PubMed  Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors thank Dr. Cecilia Bruno (Universidad Nacional de Córdoba) for her assistance in statistical data analysis, Diego Lopez Lauenstein for soil sampling, Franco Fernandez for his assistance in sequence analysis, and Paola Suarez and Daniela Gomez (IFRGV-INTA) for technical assistance. Anibal Verga, Nacira Muñoz, and Ramiro Lascano (IFRGV-INTA) critically reviewed the manuscript and helped in the discussion of data. We also thank translator Jorgelina Brasca for correcting the English style. L.C.H.D. is FCEFN–UNC student, P.G. and E.R. are INTA researchers, and M.M. is INTA and CONICET researcher. This work was supported by INTA-PNFOR-044341 and Secyt-UNC 162/12 projects. The authors declare that they have no conflict of interest.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Mariana Melchiorre.

Electronic supplementary material

Below is the link to the electronic supplementary material.

ESM 1

(DOCX 23 kb)

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Chávez Díaz, L., González, P., Rubio, E. et al. Diversity and stress tolerance in rhizobia from Parque Chaqueño region of Argentina nodulating Prosopis alba . Biol Fertil Soils 49, 1153–1165 (2013). https://doi.org/10.1007/s00374-013-0814-6

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00374-013-0814-6

Keywords

  • Prosopis alba
  • Symbiosis
  • Rhizobial diversity
  • Parque Chaqueño