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Evaluation of the mineralizable organic nitrogen in soil using an immunological technique

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Abstract

Phosphate buffer-extractable organic nitrogen (PEON), which can be extracted from soil with a 67-mM neutral phosphate buffer solution, is considered to be a major source of mineralizable nitrogen (N) that is ubiquitous in soil. We have developed a Western blot analysis and enzyme-linked immune sorbent assay (ELISA) method for determining PEON. First, anti-PEON antibody (IgG) from a rabbit was produced using purified PEON from a volcanic ash soil. PEON was detected as a ladder-like staining pattern in the high-molecular-weight region and as a single band under 14 kDa in Western blot analysis using the anti-PEON IgG. In the ELISA assay, the anti-PEON IgG could specifically react with PEON irrespective of soil type and management. Furthermore, the absorbance at 450 nm in ELISA was highly correlated to the concentration of organic N (Norg) in phosphate extracts of different soils. We propose the immune assay using the anti-PEON IgG as a possible novel method for evaluating available N in soil.

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Abbreviations

ELISA:

enzyme-linked immune sorbent assay

N:

nitrogen

Norg :

organic nitrogen

PBS:

neutral phosphate buffer solution

PEON:

neutral phosphate buffer-extractable organic nitrogen

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Authors and Affiliations

  1. Education and Research Center for Biological Resources, Shimane University, Matsue, Shimane, Japan

    Shingo Matsumoto & Junko Kasuga

  2. Biological and Environmental Science, Kobe University, Nada, Kobe, Japan

    Noriharu Ae & Mari Hirata

  3. Nippi Research Institute of Biomatrix, Senju-Midoricho, Adachi, Tokyo, Japan

    Yohichi Koyama & Katsumasa Iijima

  4. Iwate Agricultural Research Center, Kitakami, Iwate, Japan

    Rumiko Kodashima

  5. Hokkaido National Institute of Agriculture, Memuro, Kasai, Hokkaido, Japan

    Nobuhisa Koga

Authors
  1. Shingo Matsumoto
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  2. Noriharu Ae
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  3. Yohichi Koyama
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  4. Katsumasa Iijima
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  5. Rumiko Kodashima
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  6. Mari Hirata
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  7. Junko Kasuga
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  8. Nobuhisa Koga
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Correspondence to Shingo Matsumoto.

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Matsumoto, S., Ae, N., Koyama, Y. et al. Evaluation of the mineralizable organic nitrogen in soil using an immunological technique. Biol Fertil Soils 45, 107–111 (2008). https://doi.org/10.1007/s00374-008-0325-z

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  • DOI: https://doi.org/10.1007/s00374-008-0325-z

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