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Achromobacter insolitus and Zoogloea ramigera associated with wheat plants (Triticum aestivum)

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Abstract

This study reports for the first time the presence of diazotrophic bacteria belonging to the genera Achromobacter and Zoogloea associated with wheat plants. These bacterial strains were identified by the analysis of 16S rDNA sequences. The bacterium IAC-AT-8 was identified as Azospirillum brasiliense, whereas isolates IAC-HT-11 and IAC-HT-12 were identified as Achromobacter insolitus and Zoogloea ramigera, respectively. A greenhouse experiment involving a non-sterilized soil was carried out with the aim to study the endophytic feature of these strains. After 40 days from inoculation, all the strains were in the inner of roots, but they were not detected in soil. In order to assess the location inside wheat plants, an experiment was conducted under axenic conditions. Fifteen days after inoculation, preparations of inoculated plants were observed by the scanning electron microscope, using the cryofracture technique, and by the transmission electron microscope. It was observed that all isolates were present on the external part of the roots and in the inner part at the elongation region, in cortex cells, but not in the endodermis or in the vascular bundle region. No colonizing bacterial cells were observed in wheat leaves.

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Acknowledgments

The authors thank Dr. Euan James for helpful suggestions to the manuscript, the technician Mônica L. Rossi for her help in the analyses involving electron microscopy, CAPES for granting a fellowship, and to FAPESP for financial support.

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Authors and Affiliations

  1. ESALQ/USP, Av. Pádua Dias, 11, CEP-13418-900, Piracicaba, São Paulo, Brazil

    Valéria Marino Rodrigues Sala & Elke Jurandy Bran Nogueira Cardoso

  2. Divisão de Recursos Microbianos, CPQBA/UNICAMP, Cx. Postal 6171, CEP: 13081-970, Campinas, São Paulo, Brazil

    Fabiana Fantinatti Garboggini

  3. CENA/USP, Cx. Postal 96, CEP-13400-970, Piracicaba, São Paulo, Brazil

    Neusa de Lima Nogueira

  4. IAC/Centro de Solos e Recursos Ambientais, Cx. Postal 28, CEP-13010-970, Campinas, São Paulo, Brazil

    Adriana Parada Dias da Silveira

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  1. Valéria Marino Rodrigues Sala
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  2. Elke Jurandy Bran Nogueira Cardoso
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  3. Fabiana Fantinatti Garboggini
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  4. Neusa de Lima Nogueira
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  5. Adriana Parada Dias da Silveira
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Correspondence to Adriana Parada Dias da Silveira.

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Sala, V.M.R., Cardoso, E.J.B.N., Garboggini, F.F. et al. Achromobacter insolitus and Zoogloea ramigera associated with wheat plants (Triticum aestivum). Biol Fertil Soils 44, 1107–1112 (2008). https://doi.org/10.1007/s00374-008-0292-4

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  • DOI: https://doi.org/10.1007/s00374-008-0292-4

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