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Eukaryotic communities associated with the decomposition of rice straw compost in a Japanese rice paddy field estimated by DGGE analysis

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Abstract

To estimate the succession and phylogenetic composition of the eukaryotic communities responsible for the decomposition of rice straw compost under flooded conditions during the cultivation period of paddy rice, denaturing gradient gel electrophoresis (DGGE) analysis targeting 18S rDNA followed by sequencing was conducted in a Japanese paddy field. The eukaryotic communities in rice straw compost incorporated into the flooded paddy field were influenced by the mid-season drainage and mainly composed of fungi (Ascomycota, Zygomycota, and Chytridiomycota) and protozoa (Ciliophora, Euglyphida, and Dactylopodida), most of which existed continuously during the cultivation period of paddy rice. The results indicated that these eukaryotic members were associated with the decomposition of rice straw compost in paddy field soil directly or indirectly.

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References

  • Aoki S (1996) Black-Box: data analysis on the www [WWW document]. http://aoki2.si.gunma-u.ac.jp/Blackbox/Blackbox.html

  • Cahyani VR, Watanabe A, Matsuya K, Asakawa S, Kimura M (2002) Succession of microbiota estimated by phospholipid fatty acid analysis and changes in organic constituents during the composting process of rice straw. Soil Sci Plant Nutr 48:735–743

    CAS  Google Scholar 

  • Cahyani VR, Matsuya K, Asakawa S, Kimura M (2003) Succession and phylogenetic composition of bacterial communities responsible for the composting process of rice straw estimated by PCR-DGGE analysis. Soil Sci Plant Nutr 49:619–630

    CAS  Google Scholar 

  • Cahyani VR, Matsuya K, Asakawa S, Kimura M (2004) Succession and phylogenetic profile of eukaryotic communities in the composting process of rice straw estimated by PCR-DGGE analysis. Biol Fertil Soils 40:334–344

    Article  CAS  Google Scholar 

  • Darbyshire JF (1994) Soil protozoa. CAB International, Oxon

    Google Scholar 

  • Ekelund F, Ronn R (1994) Notes on protozoa in agricultural soil with emphasis on heterotrophic flagellates and naked amoebae and their ecology. FEMS Microbiol Rev 15:321–353

    Article  PubMed  CAS  Google Scholar 

  • Foissner W (1987) Soil protozoa: fundamental problems, ecological significance, adaptations in cililates and testaceans, bioindicators, and guide to the literature. Prog Protistol 2:69–212

    Google Scholar 

  • Hasebe A, Kanazawa S, Takai Y (1985) Microbial biomass in paddy soil II. “Microbial biomass carbon” measured by Jenkinson’s fumigation method. Soil Sci Plant Nutr 31:349–359

    CAS  Google Scholar 

  • Hayano K, Watanabe K, Asakawa S (1995) Behavior of selected microbial populations and their activity in rice and wheat rhizosphere and non-rhizosphere soils in long-term experimental paddy field with and without organic matter application in southwest Japan. Bull Kyushu Natl Agric Exp Stn 28:139–155 (in Japanese with English summary)

    Google Scholar 

  • James TY, Porter D, Leander CA, Vilgalys R, Longcore JE (2000) Molecular phylogenetics of the Chytridiomycota supports the utility of ultrastructural data in chytrid systematics. Can J Bot 78:336–350

    Article  CAS  Google Scholar 

  • Nannipieri P, Ascher J, Ceccherini MT, Landi L, Pietramellara G, Renella G (2003) Microbial diversity and soil functions. Eur J Soil Sci 54:655–670

    Article  Google Scholar 

  • Shiota Y, Hasegawa T, Okimura I (1987) Effect of chemical fertilizers and rice straw compost on microorganisms in paddy soils. Soil Microorganisms 29:3–9 (in Japanese with English summary)

    Google Scholar 

  • Tanahashi T, Murase J, Matsuya K, Asakawa S, Kimura M (2004) Microbial communities responsible for the decomposition of rice straw compost in a Japanese rice paddy field determined by phospholipid fatty acid (PLFA) analysis. Soil Sci Plant Nutr 50:1229–1236

    Google Scholar 

  • Tanahashi T, Murase J, Matsuya K, Hayashi M, Kimura M, Asakawa S (2005) Bacterial communities responsible for the decomposition of rice straw compost in a Japanese rice paddy field estimated by DGGE analysis of amplified 16S rDNA and 16S rRNA fragments. Soil Sci Plant Nutr 51:351–360

    Article  Google Scholar 

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Acknowledgements

We thank T. Matsuyama and M. Yamazaki in our laboratory for the helpful suggestions.

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Correspondence to Susumu Asakawa.

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Hatamoto, M., Tanahashi, T., Murase, J. et al. Eukaryotic communities associated with the decomposition of rice straw compost in a Japanese rice paddy field estimated by DGGE analysis. Biol Fertil Soils 44, 527–532 (2008). https://doi.org/10.1007/s00374-007-0239-1

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  • DOI: https://doi.org/10.1007/s00374-007-0239-1

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