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The effect of pharmaceutical waste-fungal biomass, treated to degrade DNA, on the composition of eubacterial and ammonia oxidizing populations of soil

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Abstract

The aim of this work was to study variations in the composition of eubacteria and ammonia-oxidizing populations of soil, both determined by denaturing gradient gel electrophoresis (DGGE), after the addition of a pharmaceutical fungal biomass, treated to degrade its DNA. This waste can be used as an amendment. The fungal biomass waste was added at three rates: 0.05, 0.1, and 1% per dry weight of soil. Control soil, without any amendment, was also investigated. Total DNA was extracted, purified, and amplified by using either universal (eubacteria) or specific (amoA) primers. Amplicons were separated by DGGE. Sequencing was also carried out to better assess the diversity of ammonia oxidizing bacteria. Changes in the composition of eubacterial community were detected after 3 days only in the soil treated with the highest dose, while the ammonia oxidizing population responded more promptly (after 1 day) with evident modifications at level of Nitrosolobus like sequences.

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Authors and Affiliations

  1. Dipartimento di Scienza del Suolo e Nutrizione della Pianta, Università degli Studi di Firenze, P.le delle Cascine 28, 50144, Firenze, Italy

    Maria Teresa Ceccherini, Judith Ascher, Giacomo Pietramellara & Paolo Nannipieri

  2. Istituto Sperimentale per la Nutrizione delle Piante, Sezione di Nutrizione Azotata e Microbiologia del Terreno, Via della Navicella 2/4, 00184, Roma, Italy

    Stefano Mocali

  3. Dipartimento di Biotecnologie Agrarie, Università degli Studi di Firenze, P.le delle Cascine 24, 50144, Firenze, Italy

    Carlo Viti

Authors
  1. Maria Teresa Ceccherini
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  2. Judith Ascher
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  3. Giacomo Pietramellara
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  4. Stefano Mocali
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  5. Carlo Viti
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  6. Paolo Nannipieri
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Correspondence to Maria Teresa Ceccherini.

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Ceccherini, M.T., Ascher, J., Pietramellara, G. et al. The effect of pharmaceutical waste-fungal biomass, treated to degrade DNA, on the composition of eubacterial and ammonia oxidizing populations of soil. Biol Fertil Soils 44, 299–306 (2007). https://doi.org/10.1007/s00374-007-0204-z

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  • DOI: https://doi.org/10.1007/s00374-007-0204-z

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