Abstract
In this study, we used Herbaspirillum sp. B501gfp1 (B501gfp1), an isolate from wild rice, to investigate the interaction between a non-host nitrogen-fixing endophytic bacterium and micropropagated sugarcane plants under aseptic condition. Two Japanese sugarcane plants (Saccharum sp.) cultivars (cvs) NiF8 and Ni15 were inoculated using B501gfp1 in two inoculum doses of 108 and 102 bacterial-cells-per-milliliter suspension. The results showed that bacterial cells colonized both the root and stem tissues, and colonization was apparent in the intercellular spaces. Higher bacterial numbers were detected in plant tissues inoculated with the higher inoculum concentration treatment. Bacterial numbers also varied between the two cultivars, with the higher values determined in cv Ni15. This study provides evidence that Herbaspirillum sp. B501gfp1, a rice isolate, could colonize sugarcane tissues, suggesting non-specificity of host plant among endophytes.
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Acknowledgements
This work was supported by grants from the Ministry of Education, Science, Sports and Culture of Japan and the Development of New Biorational Techniques for Sustainable Agriculture project, Ministry of Agriculture, Forestry and Fisheries, Japan. We are also grateful to Dr. Minamisawa of Graduate School of Life Science, Tohoku University for kindly providing the Herbaspirillum sp. strain B501gfp1.
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Njoloma, J., Tanaka, K., Shimizu, T. et al. Infection and colonization of aseptically micropropagated sugarcane seedlings by nitrogen-fixing endophytic bacterium, Herbaspirillum sp. B501gfp1. Biol Fertil Soils 43, 137–143 (2006). https://doi.org/10.1007/s00374-006-0078-5
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DOI: https://doi.org/10.1007/s00374-006-0078-5