Abstract
The antibiotic block technique is used to distinguish between fungal and bacterial induced activity. In the present study, the antibiotic inhibition of peptone-induced NO3− production was tested across a soil moisture gradient. Soil was incubated at 60, 80, 90 and 100% water-filled pore space (WFPS) and as a water slurry. Peptone was used as the substrate and cycloheximide and C2H2 (0.1% v/v) were added to inhibit fungal and autotrophic nitrification, respectively, the latter being considered mainly of bacterial origin. At all moisture contents is more than 80% of NO3− production was due to autotrophic nitrification. At increasing water contents the percentage of NO3− production inhibited by C2H2 increased, whereas the percentage inhibited by cycloheximide decreased from 26.4% at 60% WFPS to 4.6% in the water slurry, suggesting a different sensitivity of bacterial and fungal nitrification to soil moisture. Although no direct evidence of an alteration in the fungal population was produced in this experiment, data proved that water content influences the result of the test and hence care should be taken when comparing data using different test conditions.
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References
Anderson JPE, Domsch KH (1973) Quantification of bacterial and fungal contributions to soil respiration. Arch Mikrobiol 93:113–127
Badalucco L, Pomaré F, Grego S, Landi L (1994) Activity and degradation of streptomycin and cycloheximide in soil. Biol Fertil Soils 18:334–340
Bollag JM, Tung G (1972) Nitrous oxide release by soil fungi. Soil Biol Biochem 4:271–276
Castaldi S (1997) Microbial processes contributing to N2O production in two sandy Scottish soils. PhD thesis. Edinburgh
Castaldi S, Smith KA (1998) Effects of cycloheximide on N2O and NO3− production in a forest and an agricultural soil. Biol Fertil Soils 27:27–34
Cochrane VW (1958) Physiology of fungi. Wiley, New York
Curtis PJ (1969) Anaerobic growth of fungi. Trans Br Mycol Soc 53:299–302
Davidson EA, Swank WT, Perry TO (1986) Distinguishing between nitrification and denitrification as source of gaseous nitrogen production in soil. Appl Environ Microbiol 52:1280–1286
Hynes RK, Knowles R (1978) Inhibition by acetylene of ammonia oxidation in Nitrosomonas europea. FEMS Microbiol Lett 4:319–321
Kurokawa M, Fukumori Y, Yamanaka T (1985) A hydroxylamine-cytochrome c reductase occurs in the heterotrophic nitrifiers Arthrobacter globiformis. Plant Cell Physiol 26:1439–1442
Landi L, Badalucco L, Pomaré F, Nannipieri P (1993) Effectiveness of antibiotics to distinguish the contributions of fungi and bacteria to net nitrogen mineralization, nitrification and respiration. Soil Biol Biochem 25:1771–1778
Robertson LA, van Niel EWJ, Torremans RAM, Kuenen JG (1988) Simultaneous nitrification and denitrification in aerobic chemostat cultures of Tiosphaera pantotropha. Appl Environ Microbiol 54:2812–2818
Schimel JP, Firestone MK, Killham KS (1984) Identification of heterotrophic nitrification in a Sierran Forest soil. Appl Environ Microbiol 48:802–806
Skopp J, Jawson MD, Doran JW (1990) Steady-state aerobic microbial activity as a function of soil water content. Soil Sci Soc Am J 54:1619–1625
Stamatiadis S, Doran JW, Ingham ER (1990) Use of staining inhibitors to separate fungal and bacterial activity in soil. Soil Biol Biochem 22:81–88
Wardle DA, Parkinson D (1990) Response of the soil microbial biomass to glucose and selective inhibitors, across a soil moisture gradient. Soil Biol Biochem 22:825–834
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Castaldi, S. Cycloheximide inhibition of peptone-induced nitrate production across a soil moisture gradient. Biol Fertil Soils 41, 288–290 (2005). https://doi.org/10.1007/s00374-005-0834-y
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DOI: https://doi.org/10.1007/s00374-005-0834-y