Abstract
The influence of air-drying on dehydrogenase, invertase, β-glucosidase, urease, phosphatase, arylsulphatase and phenoloxidase activities was measured in three soils affected by anthropogenic activities and in control soils sampled from neighbouring areas. The air-drying changed enzyme activity, but the behaviour was neither site-specific nor enzyme-sensitive. Canonical discriminant analysis of enzymatic activities of both moist fresh and air-dried samples was able to discriminate between altered and unaltered soils irrespective of the site. Numerical alteration indices were developed as a linear function of the seven enzyme activities and the one obtained from fresh sample data was more successful. Air-drying apparently alters in unpredictable ways enzyme activities of soils, which could lead to misinterpretation of results.
References
Alef K, Nannipieri P (1995) Methods in applied soil microbiology and biochemistry. Academic Press, London, pp 49–116
Brohon B, Delolme C, Gourdon R (1999) Qualification of soils through microbial activities measurements: influence of the storage period on INT-reductase, phosphatase and respiration. Chemosphere 38:1973–1984
Burns RD (1978) Soil enzymes. Academic Press, London
Colombo C, Palumbo G, SanninoF, GianfredaL (2002) Chemical and biochemical indicators of managed agricultural soils. Proceedings of the 17th World Congress of Soil Science, Bangkok, Thailand. CD-ROM
Dick RP (1994) Soil enzymes activities as indicators of soil quality. In: Doran JW, Coleman DC, Bezdicek DF, Stewart BA (eds) Defining soil quality for a sustainable environment. SSSA special publication no. 35. SSSA, Madison, Wis., pp 107–124
Dick RP (1997) Soil enzyme activities as integrative indicators of soil health. In: Pankhurst CE, Doube BM, Gupta VVSR (eds) Biological indicators of soil health. CAB International, Wallingford, pp 121–156
Dick RP, Breakwell DP, Turco RF (1996) Soil enzyme activities and biodiversity measurements as integrative microbiological indicators. In: Doran JW, Jones AJ (eds) Methods for assessing soil quality. SSSA special publication no. 49. SSSA, Madison, Wis., pp 273–292
Eivazi F, Tabatabai MA (1977) Phosphatases in soils. Soil Biol Biochem 9:167–172
Eivazi F, Tabatabai MA (1990) Factors affecting glucosidase and galactosidase activities in soils. Soil Biol Biochem 22:891–897
El-Shinnawi MM, El-Shimi SA (1981) Enzymatic activities in salt affected soils. Zentralbl Mikrobiol 136:106–116
El-Shinnawi MM, Shehata SM, El-Shimi SA (1982) Enzymatic activities during incubation of remoistened soil samples stored for different periods. Zentralbl Mikrobiol 137:86–90
Frankenberger WT Jr, Bingham FT (1982) Influence of salinity on soil enzyme activities. Soil Sci Soc Am J 46:1173–1177
Gianfreda L, Bollag J-M (1996) Influence of natural and anthropogenic factors on enzyme activity in soil. In: Stotzky G, Bollag J-M (eds) Soil biochemistry, vol 9. Dekker, New York, pp 123–194
Gianfreda L, Rao MA, Sannino F, Colombo C, Palumbo G, Del Re AAM, Capri E, Puglisi E (in press) Biological and chemical indexes of soil status. Soil Biol Biochem
Kandeler E, Gerber H (1988) Short-term assay of soil urease activity using colorimetric determination of ammonium. Biol Fertil Soils 6:68–72
Ladd JN (1985) Soil enzymes. In: Vaugham D, Malcom R E (eds) Soil organic matter and biological activity. Nijhoff, Junk, Dordrecht, pp 175–221
Leiros MC, Trasar-Cepeda C, Garcia-Fernandez F, Gil-Sotres F (1999) Defining the validity of a biochemical index of soil quality. Biol Fertil Soil 30:140–146
Margesin R, Zimmerbauer A, Schinner F (2000) Monitoring of bioremediation by soil biological activities. Chemosphere 40:339–346
Nannipieri P (1994) The potential use of soil enzymes as indicators of productivity, sustainability and pollution. In: Pankhurst CE, Doube BM, Gupta VVSR, Grace PR (eds) Soil biota: management in sustainable farming systems. Adelaide CSIRO, pp 238–244
Nannipieri P, Kandeler E, Ruggiero P (2002) Enzyme activities and microbiological and biochemical processes in soil. In: Burns RG, Dick RP (eds) Enzymes in the environment. Activity, ecology and applications. Dekker, New York, pp 1–33
Pascual JA, Garcia C, Hernandez T, Moreno JL, Ros M (2000) Soil microbial activity as a biomarker of degradation and remediation processes. Soil Biol Biochem 32:1877–1883
Perucci P, Casucci C, Dumontet S (2000) An improved method to evaluate the o -diphenol oxidase activity of soil. Soil Biol Biochem 32:1927–1933
Ross DJ (1970) Effects of storage on dehydrogenase activities of soils. Soil Biol Biochem 2:55–61
Sannino F, Gianfreda L (2001) Pesticide influence on soil enzymatic activities Chemosphere 22:1–9
SAS Institute (1985) SAS user's guide: statistics, version 5. SAS, Cary, N.C.
Skujins JJ (1967) Enzymes in soils. In: McLaren AD, Peterson GH (eds) Soil biochemistry, vol 1. Dekker, New York, pp 371–414
Speir TW, Ross DJ (1975) Effects of storage on the activities of protease, urease, phosphatase and sulphatase in three soils under pasture. N Z J Sci 18:231–237
Tabatabai MA, Bremner JM (1970) Arylsulphatase activity of soils. Soil Sci Soc Am Proc 34:427–429
Tabatabai MA, Dick WA (2002) Enzymes in soil: research and developments in measuring activities In: Burns RG, Dick RP (eds) Enzymes in the environment. Activity, ecology and applications. Dekker, New York, pp 567–596
Tate RL (1995) Soil microbiology. Wiley, New York, pp 123–146
Trasar-Cepeda C, Leiros MC, Seoana S, Gil-Sotres F (2000) Limitations of soil enzymes as indicators of soil pollution. Soil Biol Biochem 32:1867–1875
Trevors JT (1984) Dehydrogenase in soil: a comparison between the INT and TTC assay. Soil Biol Biochem 16:673–674
Acknowledgements
This research was supported by the Ministero dell'Università e della Ricerca, Italy, Programmi di Interesse Nazionale PRIN 2000–2001. The authors are indebted to Attilio A.M. Del Re for critically reading the manuscript and for his advice on the statistical interpretation. DiSSPA contribution no. 048.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Rao, M.A., Sannino, F., Nocerino, G. et al. Effect of air-drying treatment on enzymatic activities of soils affected by anthropogenic activities. Biol Fertil Soils 38, 327–332 (2003). https://doi.org/10.1007/s00374-003-0660-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00374-003-0660-z