Abstract
The activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), and glutathione S-transferase (GST), as well as the concentrations of sulfhydryl (SH) groups and glutathione (GSH) were analyzed in five age classes of the Mediterranean centipede Scolopendra cingulata as follows: embryo, adolescens, maturus junior, maturus, and maturus senior. The data obtained showed the presence of SOD, CAT, GSH-Px, GR, GST, and SH groups in embryos. The transition from embryo to adolescens was accompanied by an increase in the activities of all studied enzymes, in response to the increased production of ROS due to the increased metabolic activity of the centipede associated with growth and development. Our results show that trends in antioxidant enzyme (AOE) activities were not uniform among adult age classes, suggesting that maturus junior, maturus, and maturus senior differentially respond and/or have different susceptibility to ROS. On the other hand, GSH concentration in embryos was undetectable, highest in adolescens and decreased in the latter part of life. Pearson correlation analysis in embryos showed that the activities of the AOEs were strongly and positively correlated with each other but negatively correlated with GSH and SH groups. At later age classes, SOD, CAT, GSH-Px, GR, GSH, and SH groups were no longer significantly correlated with GST. In the discriminant analysis, the variables that separated the age classes were GR, GST, SH groups, and body length. Body length was directly related to the age of individuals, clearly indicating that development/aging affects the regulation of antioxidant defense in this species.
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The datasets generated and analyzed during the current study are available from the corresponding author upon reasonable request.
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This work was supported by the Ministry of Education, Science and Technological Development of the Republic of Serbia, Contract Nos. 451-03-47/2023-01/200007 and 451-03-47/2023-01/200178. The authors are grateful to Dr. Goran Poznanović for proofreading the manuscript.
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Mitić, B.M., Borković-Mitić, S.S., Vranković, J.S. et al. Age-related changes in antioxidant defenses of the Mediterranean centipede Scolopendra cingulata (Chilopoda). J Comp Physiol B 193, 249–260 (2023). https://doi.org/10.1007/s00360-023-01481-w
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DOI: https://doi.org/10.1007/s00360-023-01481-w