Abstract
This study aimed to investigate the effects of different temperatures on seawater acclimation in rainbow trout (Oncorhynchus mykiss), in terms of growth performance, osmoregulatory capacity, and branchial phospholipid fatty acid (PLFA) composition. The fish (initial weight, 94.73 g) were reared at 9, 12.5, and 16 °C for 28 days, then acclimated to seawater, and subsequently reared for 14 days. Sampling points were on the last day in freshwater, and the 1st, 4th, 7th, and 14th days after the salinity reached 30. The results showed the final weight, percent weight gain, and specific growth rate of rainbow trout at 12.5 °C were significantly higher than those at 9 °C, while the thermal growth coefficient at 16 °C was significantly lower than that in other treatments. The branchial PLFA composition in rainbow trout changed more rapidly at 9 and 12.5 °C than at 16 °C. The branchial PLFA composition was significantly affected by temperature and salinity and their interaction. The polyunsaturated fatty acid content of phospholipids in the gill at 9 and 12.5 °C was significantly higher than those at 16 °C. Low temperature (9 °C) and seawater acclimation significantly increased the degree of unsaturation of membrane, enhancing membrane fluidity, which is related to Na+–K+ ATPase activity. Responses of plasma ion, Na+–K+ ATPase activity, and plasma glucose followed a similar pattern at different temperatures. Overall, the study suggests that 12.5 °C is the ideal temperature for seawater acclimation in rainbow trout.
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Data availability statement
The data that support the findings of this study are available from the corresponding author upon reasonable request.
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Acknowledgements
We sincerely thank Jingwen Yang, Wen Wang, and Qianlong Deng for helping collect samples. We also want to express our gratitude to You Yizhu Wang and Zongjing Deng for their assistant with biochemistry assay and fish husbandry.
Funding
This work was jointly supported by the National Key Research and Development Program of China (Project no. 2019YFD0901000), the National Natural Science Foundation of China (NSFC) (nos. 31702364 and U1906206), and the Primary Research and Development Program of Shandong Province (nos. 2018CXGC0101 and SD2019YY006).
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JG carried out the entire experiment, analyzed the data, and drafted the manuscript with the guidance of YZ and SD. MH and CH helped analyze samples. CL, QG, and YD helped edit this manuscript. YZ was responsible for the integrity of the work as a whole.
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Ethical approval for fish experiment was permitted by the Animal Experimentation Committee of the Ocean University of China (Reference Number, 2018-12).
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Ge, J., Huang, M., Zhou, Y. et al. Effects of different temperatures on seawater acclimation in rainbow trout Oncorhynchus mykiss: osmoregulation and branchial phospholipid fatty acid composition. J Comp Physiol B 191, 669–679 (2021). https://doi.org/10.1007/s00360-021-01363-z
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DOI: https://doi.org/10.1007/s00360-021-01363-z