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Ventilatory responses of the clown knifefish, Chitala ornata, to hypercarbia and hypercapnia

Journal of Comparative Physiology B volume 188, pages 581–589 (2018)Cite this article

Abstract

The aim of the present study was to determine the roles of externally versus internally oriented CO2/H+-sensitive chemoreceptors in promoting cardiorespiratory responses to environmental hypercarbia in the facultative air-breathing fish, Chitala ornata (the clown knifefish). Fish were exposed to environmental acidosis (pH ~ 6.0) or hypercarbia (≈ 30 torr PCO2) that produced changes in water pH equal to the pH levels of the acidotic water to distinguish the relative roles of CO2 versus H+. We also injected acetazolamide to elevate arterial levels of PCO2 and [H+] in fish in normocarbic water to distinguish between internal and external stimuli. We measured changes in gill ventilation frequency, air breathing frequency, heart rate and arterial blood pressure in response to each treatment as well as the changes produced in arterial PCO2 and pH. Exposure to normocarbic water of pH 6.0 for 1 h did not produce significant changes in any measured variable. Exposure to hypercarbic water dramatically increased air breathing frequency, but had no effect on gill ventilation. Hypercarbia also produced a modest bradycardia and fall in arterial blood pressure. Injection of acetazolamide produced similar effects. Both hypercarbia and acetazolamide led to increases in arterial PCO2 and falls in arterial pH although the changes in arterial PCO2/pH were more modest following acetazolamide injection as were the increases in air breathing frequency. The acetazolamide results suggest that the stimulation of air breathing was due, at least in part, to stimulation of internally oriented CO2/H+ chemoreceptors monitoring blood gas changes.

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Acknowledgements

This research was funded by the Danish Ministry of Foreign Affairs: Danida fellowship Centre Project 272 number: DFC 12-014AU iAQUA, The Danish International Development Agency (DANIDA) and by the Natural Sciences and Engineering Research Council of Canada.

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Authors and Affiliations

  1. Department of Aquatic Nutrition and Products Processing, College of Aquaculture and Fisheries, Can Tho University, Can Tho, Vietnam

    Dang Diem Tuong, Do Thi Thanh Huong & Nguyen Thanh Phuong

  2. Department of Biology, McMaster University, Hamilton, Canada

    Brittney Borowiec

  3. Department of Biological Sciences, University of Alberta, Edmonton, Canada

    Alexander M. Clifford

  4. Department of Physiological Sciences, Universidade Federal de São Carlos, São Carlos, Brazil

    Renato Filogonio

  5. Department of Zoology, University of British Columbia, Vancouver, Canada

    Alexander M. Clifford, Derek Somo & William K. Milsom

  6. Department of Bioscience, Zoophysiology, Aarhus University, Aarhus, Denmark

    Tobias Wang & Mark Bayley

  7. Aarhus Institute of Advanced Studies, 8000, Aarhus C, Denmark

    Tobias Wang

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  1. Dang Diem Tuong
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  2. Brittney Borowiec
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  3. Alexander M. Clifford
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  10. William K. Milsom
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Correspondence to William K. Milsom.

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Communicated by G. Heldmaier.

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Tuong, D.D., Borowiec, B., Clifford, A.M. et al. Ventilatory responses of the clown knifefish, Chitala ornata, to hypercarbia and hypercapnia. J Comp Physiol B 188, 581–589 (2018). https://doi.org/10.1007/s00360-018-1150-9

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  • DOI: https://doi.org/10.1007/s00360-018-1150-9

Keywords

  • Fish
  • CO2
  • Gill ventilation
  • Air breathing
  • Acidosis
  • Hypercarbia