Does diet influence salivary enzyme activities in elephant species?
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Asian elephants (Elephas maximus) and African elephants (Loxodonta africana) are herbivore generalists; however, Asian elephants might ingest a higher proportion of grasses than Africans. Although some studies have investigated nutrition-specific morphological adaptations of the two species, broader studies on salivary enzymes in both elephant species are lacking. This study focuses on the comparison of salivary enzymes activity profiles in the two elephant species; these enzymes are relevant for protective and digestive functions in humans. We aimed to determine whether salivary amylase (sAA), lysozyme (sLYS), and peroxidase (sPOD) activities have changed in a species-specific pattern during evolutionary separation of the elephant genera. Saliva samples of 14 Asian and eight African elephants were collected in three German zoos. Results show that sAA and sLYS are salivary components of both elephant species in an active conformation. In contrast, little to no sPOD activity was determined in any elephant sample. Furthermore, sAA activity was significantly higher in Asian compared with African elephants. sLYS and sPOD showed no species-specific differences. The time of food provision until sample collection affected only sAA activity. In summary, the results suggest several possible factors modulating the activity of the mammal-typical enzymes, such as sAA, sLYS, and sPOD, e.g., nutrition and sampling procedure, which have to be considered when analyzing differences in saliva composition of animal species.
KeywordsElephant Salivary enzyme activity Amylase Nutrition Evolution
For the acquisition of saliva samples from Zoo Dresden GmbH, we would like to thank Ronny Moche and David Lück, also Thomas Brockmann, Dr. Dimitri Widmer, and Dr. Eva Ziemssen for their help and support. We would like to thank Sandra Reichler, Dr. Melanie Oesterwind, and the animal caretaker Stefan Geretschläger and his team from Zoo Heidelberg GmbH. From Tierpark Berlin-Friedrichsfelde GmbH, we would like to acknowledge Dr. Günther Strauss and Mario Hammerschmidt and his team for their collaboration. For reviewing and giving helpful annotations and suggestions, we would like to thank Prof. Dr. Marcus Clauss and one anonymous reviewer. We would like to acknowledge Pamela Heidi Douglas for improving the language of this manuscript.
Compliance with ethical standards
This article does not include studies with human participants performed by any of the authors. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. The animals did not experience pain or suffering, and were not distressed by the saliva collection procedure. Therefore, the saliva collection is not an animal experiment according to German law.
Conflict of interest
The authors declare that they have no conflict of interest.
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