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Biochemical characteristics and modulation by external and internal factors of aminopeptidase-N activity in the hepatopancreas of a euryhaline burrowing crab

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Abstract

Strikingly, in spite of its physiological importance, information about occurrence, biochemical characteristics and mechanisms of regulation of aminopeptidase-N (APN) in the hepatopancreas of intertidal euryhaline crabs is still lacking. In this work, we determined the occurrence, biochemical characteristics, response to environmental salinity and dopamine of APN in the hepatopancreas of the euryhaline crab Neohelice granulata (Dana 1851) from the open mudflat of Mar Chiquita coastal lagoon (Buenos Aires province, Argentina). APN activity was maximal at pH and temperature range of 7.6–9.0 and 37–45 °C, respectively. APN activity exhibited Michaelis–Menten kinetics (apparent Km = 0.19 ± 0.10 mM) (pH 7.6, 37 °C) and appeared to be sensitive to bestatin (I 50 = 15 mM) and EDTA (I 50 = 9 mM). In crabs acclimated to 10 psu (hyper-regulation conditions) and 37 psu (hypo-regulation conditions), APN activity was about 45 and 160 % higher, respectively, than in 35 psu (osmoconformation). APN activity in the hepatopancreas was stimulated in vitro (about 137 %) by 10−4 M dopamine. Higher dopamine concentrations produced a similar extent of increase. The responses of APN activity to salinity and dopamine in vitro suggest the role of APN in digestive adjustments upon hyper and hypo-regulatory conditions and its modulation via direct mechanisms on hepatopancreas by dopamine.

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Acknowledgments

This work was partially supported by grants from the University of Mar del Plata and from Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Argentina. M.S. Michiels was supported by a Doctoral scholarship from CONICET (Argentina)

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  1. Departamento de Biología, Facultad de Ciencias Exactas y Naturales, Instituto de Investigaciones Marinas y Costeras (IIMyC), Consejo Nacional de Investigaciones Científicas y Tecnológias (CONICET), Universidad Nacional de Mar del Plata, Mar del Plata, Argentina

    M. S. Michiels, J. C. del Valle & A. A. López Mañanes

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  1. M. S. Michiels
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Michiels, M.S., del Valle, J.C. & López Mañanes, A.A. Biochemical characteristics and modulation by external and internal factors of aminopeptidase-N activity in the hepatopancreas of a euryhaline burrowing crab. J Comp Physiol B 185, 501–510 (2015). https://doi.org/10.1007/s00360-015-0899-3

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