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Modulation of redox regulatory molecules and electron transport chain activity in muscle of air breathing fish Heteropneustes fossilis under air exposure stress

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Abstract

Responses of redox regulatory system to long-term survival (>18 h) of the catfish Heteropneustes fossilis in air are not yet understood. Lipid and protein oxidation level, oxidant (H2O2) generation, antioxidative status (levels of superoxide dismutase, catalase, glutathione peroxidase and reductase, ascorbic acid and non-protein sulfhydryl) and activities of respiratory complexes (I, II, III and IV) in mitochondria were investigated in muscle of H. fossilis under air exposure condition (0, 3, 6, 12 and 18 h at 25 °C). The increased levels of both H2O2 and tissue oxidation were observed due to the decreased activities of antioxidant enzymes in muscle under water deprivation condition. However, ascorbic acid and non-protein thiol groups were the highest at 18 h air exposure time. A linear increase in complex II activity with air exposure time and an increase up to 12 h followed by a decrease in activity of complex I at 18 h were observed. Negative correlation was observed for complex III and V activity with exposure time. Critical time to modulate the above parameters was found to be 3 h air exposure. Dehydration induced oxidative stress due to modulation of electron transport chain and redox metabolizing enzymes in muscle of H. fossilis was clearly observed. Possible contribution of redox regulatory system in muscle tissue of the fish for long-term survival in air is elucidated. Results of the present study may be useful to understand the redox metabolism in muscle of fishes those are exposed to air in general and air breathing fishes in particular.

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Acknowledgments

The work was supported by the financial grant from Department of Biotechnology, Govt. of India, New Delhi under DBT-RA program at Biochemical Adaptation Laboratory, Banaras Hindu University, Varanasi India. The laboratory facilities offered by Prof. B.K. Ratha to conduct the entire work at Biochemical Adaptation Laboratory are highly acknowledged. I am also extremely grateful to the Heads, Dept. of Botany (for fluorimetric analysis in central facility) and Dept. of Zoology, BHU for providing necessary laboratory facilities. The inputs given by Prof. G.B.N. Chainy during the work are also duly acknowledged. Helps rendered by Mr. Ashok Yadav, Ms. Suman Mishra and Smita Verma during animal handling and tissue collection are highly acknowledged.

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  1. Biswaranjan Paital

    Present address: Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore, 560012, India

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  1. Biochemical Adaptation Lab., Department of Zoology, Banaras Hindu University, Varanasi, 221005, UP, India

    Biswaranjan Paital

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Communicated by G. Heldmaier.

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Paital, B. Modulation of redox regulatory molecules and electron transport chain activity in muscle of air breathing fish Heteropneustes fossilis under air exposure stress. J Comp Physiol B 184, 65–76 (2014). https://doi.org/10.1007/s00360-013-0778-8

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  • DOI: https://doi.org/10.1007/s00360-013-0778-8

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