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Acute heat stress and thermal acclimation induce CCAAT/enhancer-binding protein delta in the goby Gillichthys mirabilis

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Abstract

Members of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors have regulatory control over numerous processes related to cell fate determination, including differentiation, proliferation, cell cycle arrest and apoptosis. In mammals, abnormalities in the expression of some isoforms of C/EBPs are pathogenic and are implicated as being involved in myeloid leukemia and breast cancers. Next to nothing is known about their regulation, function or stress-responsiveness in poikilotherms. Here, both acute heat stress and thermal acclimation were demonstrated to induce the expression of one isoform, C/EBP-δ, in the liver, white muscle and gill of the eurythermal estuarine goby, Gillichthys mirabilis. The established role of C/EBP-δ in causing cell cycle arrest and/or promoting apoptosis in other vertebrates suggests that the heat-inducibility of this protein in poikilotherms may be part of the conserved cellular stress response with the hypothesized role of causing temporary cessation of cell growth and/or programmed cell death during bouts of environmental stress. The observed regulation of c/ebp-δ during hyperthermia represents a novel, heat-inducible signaling pathway in fishes.

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Acknowledgments

This research was supported by the Oregon Health Sciences University Medical Research Foundation grant #0801 to BAB. The author thanks Dr. George Somero for use of laboratory space at Stanford University’s Hopkins Marine Station for the heat exposures.

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  1. Department of Biology, Portland State University, Portland, OR, 97201, USA

    Bradley A. Buckley

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  1. Bradley A. Buckley
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Correspondence to Bradley A. Buckley.

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Communicated by H.V. Carey.

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360_2011_572_MOESM1_ESM.pdf

Fig. S1. Western blots demonstrating specificity of custom anti-C/EBP-δ antibody (Affinity Bioreagents). Fifteen μg of total protein were separated via SDS-PAGE, electrotransferred to nitrocellulose blots and probed via immunohistochemistry using the custom anti-C/EBP-δ antibody. Lane 1: pre-immune serum (negative control). Lanes 2, 3, 4: gill, white muscle and liver tissue from heat-shocked Gillichthys mirabilis, respectively. B) Lanes 1, 2, 3: gill, white muscle and liver tissue fromheat-shocked Gillichthys mirabilis separated via SDS-PAGE, electrotransferred to nitrocellulose blots and probed via immunohistochemistry using custom anti-C/EBP-δ antibody that was pre-incubated with a 200 molar excess of the antigenic C/EBP-δ peptide. (PDF 19 kb)

360_2011_572_MOESM2_ESM.pdf

Fig. S2 Levels of C/EBP-δ mRNA, histone H2B mRNA and histone H4 mRNA in the gill tissues of Gillichthys mirabilis exposed to heat shock at 32°C for a range of durations. Each datapoint represents a pooled sample from n = 4 individuals. Data are adapted from gene expression profile patterns reported in Buckley et al., 2006. (PDF 38 kb)

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Buckley, B.A. Acute heat stress and thermal acclimation induce CCAAT/enhancer-binding protein delta in the goby Gillichthys mirabilis . J Comp Physiol B 181, 773–780 (2011). https://doi.org/10.1007/s00360-011-0572-4

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