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Effect of pectin feeding on monocarboxylate transporters in rat adrenal gland

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Abstract

We have recently proved the expression and localization of seven monocarboxylate transporters (MCT1, MCT2, MCT3, MCT4, MCT5, MCT7, and MCT8) in the rat adrenal gland. So far, there are no data reporting possible regulation of any MCT isoform in the adrenal gland. Pectin is a soluble dietary fiber that is known to exert a hypocholesterolemic effect and increases the short chain fatty acids production in the large intestine. This work aimed to study the effect of pectin feeding on the expression of MCTs (MCT1–MCT5, MCT7, and MCT8) and their cellular distribution in rat adrenal gland. Western blotting demonstrated significant increase in the expression levels of MCT1, MCT2, MCT4, MCT5, and MCT7 in pectin-fed rats in comparison with the controls. Immunohistochemistry revealed extended distribution and distinctive increase in the immunoreactivities of MCT1, MCT2, MCT4, MCT5, and MCT7 in the adrenal cortical zones, besides the increase in the immunoreactive intensity of MCT5 and MCT7 in the adrenal medulla of pectin-fed versus control rats. Interestingly, zona glomerulosa which did not show any reactivity for MCT1 or MCT2 in controls, exhibited marked immunopositivities for both MCT1 and MCT2 in pectin-fed rats. MCT3 and MCT8, however, did not show significant changes in their expression levels between pectin-fed and control rats. Our data is the first to describe the up regulation of various MCTs in rat adrenal gland under the influence of pectin feeding. This up regulation might be a compensatory response to the hypocholesterolemic effect of pectin in order to maximize the intracellular availability of acetate. This article suggests that monocarboxylate transporters have an important physiological role in the regulation of adrenal hormones as well as in cholesterol homeostasis.

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References

  • Andersen JM, Dietschy JM (1976) Regulation of sterol synthesis in adrenal gland of the rat by both high and low density human lipoproteins. Biochem Biophys Res Commun 72:880–885

    Article  CAS  PubMed  Google Scholar 

  • Andersen JM, Dietschy JM (1978) Relative importance of high and low density lipoproteins in the regulation of cholesterol synthesis in the adrenal gland, ovary, and testis of the rat. J Biol Chem 253:9024–9032

    CAS  PubMed  Google Scholar 

  • Anderson JW, Deakins DA, Floore TL, Smith BM, Whitis SE (1990) Dietary fiber and coronary heart disease. Crit Rev Food Sci Nutr 29:95–147

    Article  CAS  PubMed  Google Scholar 

  • Arjmandi BH, Craig J, Nathani S, Reeves RD (1992) Soluble dietary fiber and cholesterol influence in vivo hepatic and intestinal cholesterol biosynthesis in rats. J Nutr 122:1559–1565

    CAS  PubMed  Google Scholar 

  • Balasubramaniam S, Goldstein JL, Faust JR, Brunschede GY, Brown MS (1977) Lipoprotein-mediated regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase activity and cholesteryl ester metabolism in the adrenal gland of the rat. J Biol Chem 252:1771–1779

    CAS  PubMed  Google Scholar 

  • Brydon WG (1995) In vitro methods that anticipate the colonic influence of dietary fiber. In: Kritchevsky D, Bonefield C (eds) Dietary fiber in health and disease. Eagan Press, St Paul, MN, pp 346–352

    Google Scholar 

  • Capponi AM (2002) Regulation of cholesterol supply for mineralocorticoid biosynthesis. Trends Endocrinol Metab 13:118–121

    Article  CAS  PubMed  Google Scholar 

  • D’Angelo SA, Grodin JM (1964) Experimental hyperthyroidism and adrenocortical function in the rat. Endocrinology 74:509–514

    Article  Google Scholar 

  • Donald RA, Espiner EA (1975) The plasma cortisol and corticotropin response to hypoglycemia following adrenal steroid and ACTH administration. J Clin Endocrinol Metab 41:1–6

    Article  CAS  PubMed  Google Scholar 

  • Flourie B, Vidon N, Florent CH, Bernier JJ (1984) Effect of pectin on jejunal glucose absorption and unstirred layer thickness in normal man. Gut 25:936–941

    Article  CAS  PubMed  Google Scholar 

  • Friesema EC, Ganguly S, Abdalla A, Manning Fox JE, Halestrap AP, Visser TJ (2003) Identification of monocarboxylate transporter 8 as a specific thyroid hormone transporter. J Biol Chem 278:40128–40135

    Article  CAS  PubMed  Google Scholar 

  • Fukunaga T, Sasaki M, Araki Y, Okamoto T, Yasuoka T, Tsujikawa T, Fujiyama Y, Bamba T (2003) Effects of the soluble fibre pectin on intestinal cell proliferation, fecal short chain fatty acid production and microbial population. Digestion 67:42–49

    Article  CAS  PubMed  Google Scholar 

  • Fuse K, Bamba T, Hosoda S (1989) Effects of pectin on fatty acid and glucose absorption and on thickness of unstirred water layer in rat and human intestine. Dig Dis Sci 34:1109–1116

    Article  CAS  PubMed  Google Scholar 

  • Garcia-Diez F, Garcia-Mediavilla V, Bayon JE, Gonzalez-Gallego J (1996) Pectin feeding influences fecal bile acid excretion, hepatic bile acid and cholesterol synthesis and serum cholesterol in rats. J Nutr 126:1766–1771

    CAS  PubMed  Google Scholar 

  • Gomathy R, Vijayalekshmi NR, Kurup PA (1990) Hypoglycemic action of the pectin present in the juice of the inflorescence stalk of plantain (Musa sapientum)—mechanism of action. J Biosci 15:297–303

    Article  CAS  Google Scholar 

  • Halestrap AP, Meredith D (2004) The SLC16 gene family-from monocarboxylate transporters (MCTs) to aromatic amino acid transporters and beyond. Pflugers Arch Eur J Physiol 447:619–628

    Article  CAS  Google Scholar 

  • Ismahan G, Parvez H, Parvez S (1977) Effects of thyroidectomy and l-thyroxine on adrenaline and noradrenaline concentrations in the adrenal glands and plasma of rats during the pro-oestrous phase of the oestrous cycle and pregnancy. Br J Pharmacol 59:275–281

    CAS  PubMed  Google Scholar 

  • Johnson EO, Kamilaris TC, Calogero AE, Gold PW, Chrousos GP (2005) Experimentally-induced hyperthyroidism is associated with activation of the rat hypothalamic-pituitary-adrenal axis. Eur J Endocrinol 153:177–185

    Article  CAS  PubMed  Google Scholar 

  • Judd PA, Truswell AS (1982) Comparison of the effects of high- and low-methoxyl pectins on blood and faecal lipids in man. Br J Nutr 48:451–458

    Article  CAS  PubMed  Google Scholar 

  • Judd PA, Truswell AS (1985) The hypocholesterolaemic effects of pectins in rats. Br J Nutr 53:409–425

    Article  CAS  PubMed  Google Scholar 

  • Kim DK, Kanai Y, Chairoungdua A, Matsuo H, Cha SH, Endou H (2001) Expression cloning of a Na+-independent aromatic amino acid transporter with structural similarity to H+/monocarboxylate transporters. J Biol Chem 276:17221–17228

    Article  CAS  PubMed  Google Scholar 

  • Kirat D, Inoue H, Iwano H, Yokota H, Taniyama H, Kato S (2007a) Monocarboxylate transporter 1 (MCT1) in the liver of pre-ruminant and adult bovines. Vet J 173:124–130

    Article  CAS  PubMed  Google Scholar 

  • Kirat D, Matsuda Y, Yamashiki N, Hayashi H, Kato S (2007b) Expression, cellular localization, and functional role of monocarboxylate transporter 4 (MCT4) in the gastrointestinal tract of ruminants. Gene 391:140–149

    Article  CAS  PubMed  Google Scholar 

  • Kirat D, Sallam K, Hayashi H, Miyasho T, Kato S (2009) Presence of ten isoforms of monocarboxylate transporter (MCT) family in the bovine adrenal gland. Mol Cell Endocrinol 298:89–100

    Article  CAS  PubMed  Google Scholar 

  • Kumar GP, Sudheesh S, Vijayalakshmi NR (1993) Hypoglycemic effect of Coccinia indica: mechanism of action. Planta Med 59:330–332

    Article  CAS  PubMed  Google Scholar 

  • Langley K, Hepp R, Grant NJ, Aunis D, Rodriguez-Pena A (2002) Thyroid hormones regulate adrenal chromaffin cell SNAP-25. Ann NY Acad Sci 971:277–280

    Article  CAS  PubMed  Google Scholar 

  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275

    CAS  PubMed  Google Scholar 

  • Lupton JR, Kurtz PP (1993) Relationship of colonic luminal short-chain fatty acids and pH to in vivo cell proliferation in rats. J Nutr 123:1522–1530

    CAS  PubMed  Google Scholar 

  • Millaruelo AI, Sagarra MR, Delicado E, Torres M, Miras-Portugal MT (1986) Enzymes and pathways of glucose utilization in bovine adrenal medulla. Mol Cell Biochem 70:67–76

    Article  CAS  PubMed  Google Scholar 

  • Poluektov AI (1964) Effect of thyroid hormone on regeneration of the adrenal cortex. Bull Exp Biol Med 57:88–91

    Article  CAS  Google Scholar 

  • Roy CC, Kien CL, Bouthillier L, Levy E (2006) Short-chain fatty acids: ready for prime time? Nutr Clin Pract 21:351–366

    Article  PubMed  Google Scholar 

  • Widmaier EP, Plotsky PM, Sutton SW, Vale WW (1988) Regulation of corticotropin-releasing factor secretion in vitro by glucose. Am J Physiol 255:287–292

    Google Scholar 

Download references

Acknowledgments

The author would like to thank Professor Seiyu Kato (Rakuno Gakuen University) for his helpful discussion and excellent suggestions. The present study was supported by the Japan Society for the Promotion of Science (JSPS).

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Authors and Affiliations

  1. Department of Veterinary Physiology, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido, 069-8501, Japan

    Doaa Kirat

  2. Department of Physiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, 44519, Egypt

    Doaa Kirat

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Correspondence to Doaa Kirat.

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Communicated by I. D. Hume.

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Kirat, D. Effect of pectin feeding on monocarboxylate transporters in rat adrenal gland. J Comp Physiol B 180, 57–65 (2010). https://doi.org/10.1007/s00360-009-0382-0

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  • DOI: https://doi.org/10.1007/s00360-009-0382-0

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