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Dietary nutrient levels regulate protein and carbohydrate intake, gluconeogenic/glycolytic flux and blood trehalose level in the insect Manduca sexta L.

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Abstract

This study examined the effects of dietary casein and sucrose levels on nutrient intake, and distinguished the effects of carbohydrate and protein consumption on growth, fat content, pyruvate metabolism and blood trehalose level of 5th instar Manduca sexta larvae. Growth increased with increasing casein consumption but was unaffected by carbohydrate intake. Fat content also increased with carbohydrate consumption, but on carbohydrate-free diets fat content increased with increased protein consumption. Blood trehalose level and pyruvate metabolism were examined by nuclear magnetic resonance spectroscopy analysis of blood following administration of (3-13C)pyruvate. On diets containing sucrose, blood trehalose increased with increasing carbohydrate intake, and on most diets trehalose was synthesized entirely from dietary sucrose. Pyruvate cycling, indicated by the alanine C2/C3 13C enrichment ratio, increased with carbohydrate consumption reflecting increased glycolysis, and pyruvate decarboxylation exceeded carboxylation on all sucrose diets. Larvae that consumed <75 mg/day sucrose were gluconeogenic, based on the [2 (trehalose C6)(Glx C3/C2)]/alanine C2] 13C enrichment ratio. On carbohydrate-free diets, blood trehalose levels were low and maintained entirely by gluconeogenesis. Blood trehalose level increased with increasing protein intake. Pyruvate cycling was very low, although many insects displayed higher levels of pyruvate decarboxylation than carboxylation. All gluconeogenic larvae displayed alanine 13C enrichment ratios <0.35 and had blood trehalose levels <50 mM.

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Abbreviations

Gl x :

∑glutamine+glutamate+γ glutamyl moiety of glutathione

NMR :

nuclear magnetic resonance spectroscopy

TCA :

tricarboxylic acid

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Acknowledgements

The experiments were conducted in accordance with current US laws.

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Authors and Affiliations

  1. Analytical Chemistry Instrumentation Facility, University of California, Riverside, CA 92521, USA

    S. N. Thompson & D. B. Borchardt

  2. Department of Entomology, University of California, Riverside, CA 92521, USA

    S. N. Thompson & L.-W. Wang

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  1. S. N. Thompson
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  2. D. B. Borchardt
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  3. L.-W. Wang
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Correspondence to S. N. Thompson.

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Communicated by L.C.–H. Wang

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Thompson, S.N., Borchardt, D.B. & Wang, LW. Dietary nutrient levels regulate protein and carbohydrate intake, gluconeogenic/glycolytic flux and blood trehalose level in the insect Manduca sexta L.. J Comp Physiol B 173, 149–163 (2003). https://doi.org/10.1007/s00360-002-0322-8

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