Auditory brainstem responses in the bat Carollia perspicillata: threshold calculation and relation to audiograms based on otoacoustic emission measurement

Abstract

An objective method to evaluate auditory brainstem-evoked responses (ABR) based on the root-mean-square (rms) amplitude of the measured signal and bootstrapping procedures was used to determine threshold curves (see Lv et al. in Med Eng Phys 29:191–198, 2007; Linnenschmidt and Wiegrebe in Hear Res 373:85–95, 2019). The rms values and their significance for threshold determination depended strongly on the filtering of the signal. Using the minimum threshold values obtained at three different low-frequency filter corner frequencies (30, 100, 300 Hz), ABR threshold curves were calculated. The course of the ABR thresholds was comparable to that of published DPOAE (distortion-product otoacoustic emission) thresholds based on a − 10 dB SPL threshold criterion for the 2f1f2 emission (Schlenther et al. in J Assoc Res Otolaryngol 15:695–705, 2014, frequency range 10–90 kHz). For frequencies between 20 and 80 kHz, which is the most sensitive part of the bat’s audiogram, median thresholds ranged between 10 and 28 dB SPL, and the DPOAE thresholds ranged between 10 and 23 dB SPL. At frequencies below 20 kHz (5–20 kHz) and above 80 kHz (80–120 kHz), ABR thresholds increased by 20 dB/octave and 45 dB/octave, respectively. We conclude that the combination of objective threshold determination and multiple filtering of the signal gives reliable ABR thresholds comparable to cochlear threshold curves.

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Abbreviations

ABR:

Auditory brainstem response

DPOAE:

Distortion-product otoacoustic emission

rms:

Root mean square

References

  1. Ammersdörfer S, Galinski S, Esser K-H (2012) Effects of aversive experience on the behavior within a custom-made plus maze in the short-tailed fruit bat, Carollia perspicillata. J Comp Physiol A 98:733–739

    Article  Google Scholar 

  2. Boku S, Riquimaroux H, Simmons AM, Simmons JA (2015) Auditory brainstem response of the Japanese house bat (Pipistrellus abramus). J Acoust Soc Am 137:1063–1068

    Article  Google Scholar 

  3. Dobie RA, Wilson MJ (1989) Analysis of auditory evoked potentials by magnitude-squared coherence. Ear Hear 10:2–13

    CAS  Article  Google Scholar 

  4. Eckrich T, Foeller E, Stuermer IW, Gaese BH, Kössl M (2008) Strain-dependence of age-related cochlear hearing loss in wild and domesticated Mongolian gerbils. Hear Res 235:72–79

    Article  Google Scholar 

  5. Elberling C, Don M (1984) Quality estimation of averaged auditory brainstem responses. Scand Audiol 13:187–197

    CAS  Article  Google Scholar 

  6. Esser K-H, Eiermann A (1999) Tonotopic organization and parcellation of auditory cortex in the FM-bat Carollia perspicillata. Eur J Neurosci 11:3669–3682

    CAS  Article  Google Scholar 

  7. García-Rosales F, Martin LM, Beetz MJ, Cabral-Calderin Y, Kössl M, Hechavarría J (2018) Low-frequency spike-field coherence is a fingerprint of periodicity coding in the auditory cortex. iScience 9:47-62

    Article  Google Scholar 

  8. Hechavarría JC, Macías S, Vater M, Voss C, Mora EC, Kössl M (2013) Blurry topography for precise target-distance computations in the auditory cortex of echolocating bats. Nat Comms 4(2587):1–11

    Google Scholar 

  9. Hechavarría JC, Beetz MJ, Macias S, Kössl M (2016) Vocal sequences suppress spiking in the bat auditory cortex while evoking concomitant steady-state local field potentials. Sci Rep 6:39226

    Article  Google Scholar 

  10. Heffner RS, Koay G, Heffner HE (2019) Bats are unusually insensitive to brief low-frequency tones. J Comp Physiol A 205:583–594

    Article  Google Scholar 

  11. Koay G, Heffner RS, Bitter KS, Heffner HE (2003) Hearing in American leafnosed bats. II. Carollia perspicillata. Hear Res 178:27–34

    Article  Google Scholar 

  12. Kössl M (1992) High frequency distortion products from the ears of two bat species, Megaderma lyra and Carollia perspicillata. Hear Res 60:156–164

    Article  Google Scholar 

  13. Kössl M, Frank G, Faulstich M, Russell IJ (1999) Evolutionary adaptations of inner ear function in Jamaican mormoopid bats. J Comp Physiol A 185:217–228

    Article  Google Scholar 

  14. Land R, Burghard A, Kral A (2016) The contribution of inferior colliculus activity to the auditory brainstem response (ABR) in mice. Hear Res 341:109–118

    Article  Google Scholar 

  15. Linnenschmidt M, Wiegrebe L (2019) Ontogeny of auditory brainstem responses in the bat, Phyllostomus discolor. Hear Res 373:85–95

    Article  Google Scholar 

  16. Long GR, Talmadge CL, Lee J (2008) Measuring distortion product otoacoustic emissions using continuously sweeping primaries. J Acoust Soc Am 124:16414

    Google Scholar 

  17. Lv J, Simpson DM, Bell SL (2007) Objective detection of evoked potentials using a bootstrap technique. Med Eng Phys 29:191–198

    Article  Google Scholar 

  18. Müller M, Smolders JWT, Meyer AM, zum Gottesberge I, Reuter A, Zwacka RM, Weiher H, Klinke R (1997) Loss of auditory function in transgenic Mpvl7-deficient mice. Hear Res 114:259–263

    Article  Google Scholar 

  19. Ramsier MA, Dominy NJ (2010) A Comparison of auditory brainstem responses and behavioral estimates of hearing sensitivity in Lemur catta and Nycticebus coucang. Am J Primatol 72:217–233

    Article  Google Scholar 

  20. Schlenther D, Voss C, Kössl M (2014) Influence of ketamine-xylazine anaesthesia on cubic and quadratic high-frequency distortion-product otoacoustic emissions. J Assoc Res Otolaryngol 15:695–705

    CAS  Article  Google Scholar 

  21. Sterbing SJ (2002) Postnatal development of vocalizations and hearing in the phyllostomid bat, Carollia perspicillata. J Mammal 83:516–525

    Article  Google Scholar 

  22. Suthakar K, Liberman MC (2019) A simple algorithm for objective threshold determination of auditory brainstem responses. Hear Res 381:107782

    Article  Google Scholar 

  23. Szymanski MD, Bain DE, Kiehl K, Pennington S, Wong S, Henry KR (1999) Killer whale (Orcinus orca) hearing: auditory brainstem response and behavioral audiograms. J Acoust Soc Am 106:1134–1141

    CAS  Article  Google Scholar 

  24. Wenstrup JJ (1984) Auditory sensitivity in the fish-catching bat, Noctilio leporinus. J Comp Physiol A 155:91–101

    Article  Google Scholar 

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Acknowledgements

This study was supported by the Deutsche Forschungsgemeinschaft (KO 987 14/1). The animals used in this study come from our own breeding colony. All experimental procedures were in compliance with current German laws on animal experimentation and were approved by the Regierungspräsidium Darmstadt (permit FR/1010).

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Correspondence to Manfred Kössl.

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Wetekam, J., Reissig, C., Hechavarria, J.C. et al. Auditory brainstem responses in the bat Carollia perspicillata: threshold calculation and relation to audiograms based on otoacoustic emission measurement. J Comp Physiol A 206, 95–101 (2020). https://doi.org/10.1007/s00359-019-01394-6

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Keywords

  • Auditory system
  • Echolocation
  • Cochlea
  • ABR
  • Distortion products