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Protektive Wirkung Blaulicht absorbierender IOLs auf das menschliche retinale Pigmentepithel

Protective effect of blue light-absorbing IOLs on the human retinal pigment epithelium

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Zusammenfassung

Hintergrund

Kumulative Lichtexposition wird für die Entstehung der altersabhängige Makuladegeneration (AMD) mitverantwortlich gemacht. Um die Netzhaut vor den schädlichen Einflüssen des blauen Lichts zu schützen, werden seit geraumer Zeit Blaulicht absorbierende Intraokularlinsen (IOLs) eingesetzt. Diese Studie vergleicht mögliche zytoprotektive Effekte der Blaulicht absorbierenden SN60AT IOL (ALCON) mit der ungefärbten, UV-Licht absorbierenden SA60AT IOL (ALCON) auf das humane retinale Pigmentepithel (RPE).

Methoden

Kulturen primärer humaner RPE Zellen wurden mit weißem Licht bestrahlt, das entweder durch eine SN60AT IOL oder eine SA60AT IOL gefiltert wurde. Nach einer Bestrahlungszeit von 15–60 min wurden Vitalität, Induktion von Apoptose und die Expression des „vascular endothelial growth factor A“ (VEGF-A) sowie des antiapoptotisch wirksamen Proteins XIAP auf zellulärer Ebene bestimmt (RT-PCR/Western Blot/ELISA)

Ergebnisse

Die ungefilterte Bestrahlung der RPE-Zellen mit weißem Licht führte zu einer bestrahlungszeitabhängigen Abnahme der Vitalität und der Expression von XIAP sowie zu einer Induktion von Apoptose und Bildung von VEGF-A. Diese phototoxische Zellschädigung wurde durch beide untersuchten IOLs signifikant reduziert. Die Zellschädigung durch Bestrahlung mit Licht nach Blaulichtfilterung mit der SN60AT IOL war signifikant geringer als mit der SA60AT IOL.

Schlussfolgerung

Sowohl UV-filternde als auch Blaulicht absorbierende IOLs reduzieren phototoxische RPE-Schädigung. Die Blaulicht absorbierende SN60AT IOL führte in unseren Versuchen zu einer zusätzlichen Reduktion der Zellschädigung. Die Ergebnisse deuten darauf hin, dass Blaulicht absorbierende IOLs auch im klinischen Gebrauch eine photoprotektive Wirksamkeit auf Netzhautebene besitzen.

Abstract

Methods

Primary human RPE cells were exposed to white light and either a SN60AT or SA60AT IOL was placed in the light beam. After 15–60 min of irradiation, viability, induction of apoptosis and cell death were determined in primary human RPE cells. Expression of vascular endothelial growth factor A (VEGF-A) and the anti-apoptotic XIAP protein and their mRNA were determined by RT-PCR, Western blot analysis and ELISA.

Results

Light exposure decreased cell viability depending on the duration of irradiation. Light-induced cell death and apoptosis as well as decrease of XIAP expression and cellular viability were significantly reduced by both the SN60AT and SA60AT IOL. In addition, these protective effects regarding light-induced cell damage were significantly stronger in the presence of the blue light-filtering SN60AT IOL compared to the SA60AT IOL.

Conclusion

Both UV-filtering and blue light-absorbing IOLs reduce light-induced RPE damage. The blue light-absorbing IOL further reduced damage compared to the conventional IOL, which supports the hypothesis of possibly also preventing retinal damage in clinical use.

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  1. Augenklinik der Ludwig-Maximilians-Universität, Mathildenstraße 8, 80336, München, Deutschland

    M. Kernt, C. Hirneiss, A.S. Neubauer, C.A. Lackerbauer, K.H. Eibl, A. Wolf, MW. Ulbig & A. Kampik

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  1. M. Kernt
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  2. C. Hirneiss
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  3. A.S. Neubauer
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  4. C.A. Lackerbauer
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  5. K.H. Eibl
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  6. A. Wolf
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  7. MW. Ulbig
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Correspondence to M. Kernt.

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Kernt, M., Hirneiss, C., Neubauer, A. et al. Protektive Wirkung Blaulicht absorbierender IOLs auf das menschliche retinale Pigmentepithel. Ophthalmologe 107, 150–157 (2010). https://doi.org/10.1007/s00347-009-1945-x

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  • DOI: https://doi.org/10.1007/s00347-009-1945-x

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