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Vitreal induzierte RPE-Zell-Traktion

Untersuchung pathologischer Glaskörperproben in einem In-vitro-Kontraktionsmodell

Vitreal-induced RPE cell traction

Investigation of pathological vitreous samples in an in vitro contraction model

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Zusammenfassung

Hintergrund

Zielsetzung dieser Studie war, die Kontraktilität von retinalen Pigmentepithel- (RPE-)Zellen zu quantifizieren, die durch bioaktive Faktoren pathologischer Glaskörperproben induziert wird.

Material und Methoden

Unter Verwendung eines In-vitro-Kontraktionsmodells konnten die Aktivität von Glaskörperproben unterschiedlicher vitreoretinaler Erkrankungen untersucht werden. Dazu wurden transdifferentierte porcine RPE-Zellen auf hemisphärische Typ-I-Kollagen-Gele aufgebracht. Nach Zugabe der Glaskörperproben [physiologisch (n=6); rhegmatogene Ablatio (n=11); proliferative Vitreoretinopathie (PVR, n=10); proliferative diabetische Retinopathie (PDR, n=6)] wurde die induzierte Gelkontraktion bestimmt.

Ergebnisse

Die spezifische Aktivität der unklassifizierten Proben betrug 0,04 (Median, Spannweite, SW: 0–0,08). Glaskörperproben, die von Patienten mit verschiedenen PVR-Stadien gewonnen wurden, wiesen eine spezifische Aktivität von 0,45 (Median, SW: 0,03–1,45) auf. Die Glaskörperproben von rhegmatogen bedingten Netzhautablösungen zeigten eine Aktivität von 0,13 (Median, SW: 0,01–0,93). Die spezifische Aktivität von Glaskörperproben von Patienten mit diabetischer Retinopathie betrug 0,17 (Median, SW: 0,06–0,29). Die mittlere spezifische Aktivität und Gesamtaktivität dieser Gruppen waren signifikant im Vergleich zu den unklassifizierten Proben bzw. den Basiswerten erhöht (p<0,05).

Schlussfolgerung

Pathologische Glaskörperproben unterschiedlicher vitreoretinaler Erkrankungen enthalten ausreichende Mengen von biologisch aktiven Substanzen, die eine Kontraktion von Extrazellulärmatrix induzieren können.

Abstract

Background

The aim of this study was to quantify the contraction of retinal pigment epithelium cells (RPE) induced by bioactive factors in pathological vitreous samples.

Material and methods

Using an in vitro contraction assay, the contraction-stimulating activity of vitreous samples of different vitreoretinal pathologies was evaluated. Transdifferentiated porcine RPE cells were placed on hemispherical type I collagen gel. After exposure to pathological vitreous samples derived from different entities (physiological (n=6), rhegmatogenous retinal detachment (n=11), proliferative vitreoretinopathy (PVR) (=10), proliferative diabetic retinopathy (n=6)) the induced gel contraction was determined.

Results

The specific activity of the unclassified samples was 0.04 (median, range: 0-0.08). Vitreous samples derived from patients diagnosed as having any grade of PVR displayed a specific activity of 0.45 (median, range: 0.03-1.45). Samples removed from patients with rhegmatogenous retinal detachment disclosed a specific activity of 0.13 (median, range: 0.01-0.93). The specific activity of vitreous samples removed from patients with diabetic retinopathy had a specific activity of 0.17 (median, range: 0.06-0.29). The mean specific and total activities of these groups were significantly elevated above the unclassified or baseline values (p<0.05).

Conclusion

Pathological vitreous samples of different vitreoretinal pathologies contain sufficient amounts of biologically active factors to induce extracellular matrix contraction.

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Authors and Affiliations

  1. Klinik für Augenheilkunde, Universitätsklinikum Schleswig-Holstein, Campus Lübeck, Ratzeburger Allee 160, 23538, Lübeck, Deutschland

    J. Beutel, M. Lüke &  S. Grisanti

  2. Universitätsaugenklinik Tübingen, Abteilung I, Eberhard-Karls-Universität Tübingen, Tübingen, Deutschland

    K.-U. Bartz-Schmidt

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  1. J. Beutel
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  2. M. Lüke
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  3. K.-U. Bartz-Schmidt
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  4. S. Grisanti
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Correspondence to S. Grisanti.

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Beutel, J., Lüke, M., Bartz-Schmidt, KU. et al. Vitreal induzierte RPE-Zell-Traktion. Ophthalmologe 106, 893–898 (2009). https://doi.org/10.1007/s00347-008-1847-3

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  • DOI: https://doi.org/10.1007/s00347-008-1847-3

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