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A preliminary study on the role of Bacteroides fragilis in stent encrustation

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Abstract

Objective

To preliminarily study the characteristics of bacterial flora distribution in the urine of ureteral stent encrustation patients as well as the relation between Bacteroides and stent encrustation.

Methods

Patients undergoing ureteral stenting were included in the study and divided into encrustation group and non-encrustation group based on the condition of stent encrustation. The urine of patients was collected to undergo 16s DNA test to compare the bacterial flora distribution characteristics of the two groups. The bacterial genus with highest abundance in the urine of encrustation group was used for animal experiment. A rat model with a foreign body in the bladder was created, in which the rats were injected with the aforesaid bacterial genus. A control group injected with normal saline was also formed. The incidence of foreign body tube encrustation between the two groups was compared.

Results

The urine collected from the patients in encrustation group contained a variety of bacteria, while dominant bacteria genera included g_Lactobacillus (23.1%), g_Bacteroides (18.8%) and g_norank_Bacteroides (17.1%). While the urine from the non-encrustation group was less diverse in bacteria flora, as the major bacteria genera were g_Escherichia–Shigella (32.2%), g_Enterococcus (24.9%) and g_Pseudomonas (18.2%). Bacteroidetes in the encrustation group were significantly higher, therefore Bacteroides fragilis in this genus was adopted for animal experiment, resulting in a higher incidence of foreign body tube encrustation in the bladder among rats.

Conclusion

The present study enriches our knowledge about ureteral stent encrustation and reveals that the target regulation of urine bacteria is worth further research and clinical application.

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Acknowledgements

This study was supported by grants from the Shandong Medical and Health Technology Development Project (2018WS288) and Shandong Medical and Health Technology Development Project (2018WS291).

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Author notes

  1. Xiaopeng Li and Han Gao contributed equally to this work.

Authors and Affiliations

  1. Department of Urology, Shandong Provincial Third Hospital, No. 12, Wuyingshan Middle Road, Jinan, Shandong, China

    Xiaopeng Li, Han Gao, Wei Wei & Yongliang Ni

  2. Department of Urology, Qilu Hospital of Shandong University, Jinan, Shandong, China

    Xiaopeng Li, Han Gao, Xiaolu Sun & Yan Li

  3. Department of Urology, Jinan Central Hospital, Jinan, Shandong, China

    Zhongxian Huang

  4. Department of Urology, Jinan Jigang Hospital, Jinan, Shandong, China

    Bo Wang

  5. Yabo Biotechnology Co., Ltd., Jinan, Shandong, China

    Changjuan Wang

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  1. Xiaopeng Li
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Contributions

WW and NYL designed the research; LXP, GH, SXL, HZX, WCJ and WB conducted the studies; LY and NYL analyzed the data and prepared the manuscript; W.W. guided the experiments and edited the paper. All authors read and approved the manuscript.

Corresponding authors

Correspondence to Wei Wei or Yongliang Ni.

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Conflict of interest

The authors declare that they have no competing interests.

Data available statement

The datasets generated and analyzed during the current study are not publicly available as the experimental data are related to other experiments that are progressing, but are available from the corresponding author on reasonable request.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the Shandong Provincial Third Hospital Medical Ethics Committee (KYLL-2018003) and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. This article does not contain any studies with animals performed by any of the authors. All animal experiments complied with the ARRIVE guidelines, were performed in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals (NIH publications no. 8023, revised 1978) and were approved by the Ethics Committee on the Care and Use of Laboratory Animals (approval no. 2019076), Qilu Hospital, Shandong University, Jinan, P. R. China.

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Informed consent was obtained from all individual participants included in the study.

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Li, X., Gao, H., Sun, X. et al. A preliminary study on the role of Bacteroides fragilis in stent encrustation. World J Urol 39, 579–588 (2021). https://doi.org/10.1007/s00345-020-03185-0

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  • DOI: https://doi.org/10.1007/s00345-020-03185-0

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