Relation of ADRB3, GEF, ROCK2 gene polymorphisms to clinical findings in overactive bladder

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Abstract

Purpose

Adrenergic and cholinergic pathways play an important role in contraction–relaxation harmony in human bladder. Functional changes in any proteins in these pathways may result in overactive bladder. We aimed to investigate whether single gene polymorphisms affecting adrenergic and cholinergic pathways are associated with OAB syndrome.

Methods

60 patients with idiopathic OAB and 60 healthy controls were included in the study. A validated OAB-V8 questionnaire was given to all patients. Polymorphisms of ADRB3, ROCK2, and GEF gene were detected by PCR from whole blood samples. Genotypic structures of patients and controls were compared. The relationship between genotypic structures and OAB symptom scores were investigated.

Results

We found no significant difference in the genotype and allele frequencies between the patients and controls for all three SNP. While there was no relationship between ADRB3 and GEF gene polymorphisms and OAB scores in OAB patients, the OAB score in heterozygous polymorphic individuals was significantly higher than in homozygous polymorphic individuals in the ROCK2 gene (p = 0.039).

Conclusion

The polymorphisms of the ADRB3, ROCK2, and GEF genes were present in both OAB group and healthy controls, but were not associated with OAB syndrome.

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Acknowledgements

This study was supported by the Pamukkale University Scientific Research Projects Department.

Funding

This study was funded by Pamukkale University Scientific Research Projects Department (Grant number 2017TIPF008).

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Authors

Contributions

EF project development, data collection and management, data analysis, manuscript writing. ZA project development, data collection and management, manuscript editing. ŞA project development, data analysis. KK data collection. HA data analysis. HA project development, data management, data analysis, manuscript editing.

Corresponding author

Correspondence to Elif Fırat.

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The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional research committee (Pamukkale University Non-Interventional Clinical Research Ethics Committee; Reference number: 04; Date: 20.03.2017) and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Informed consent was taken from all human participants included in this study.

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Fırat, E., Aybek, Z., Akgün, Ş. et al. Relation of ADRB3, GEF, ROCK2 gene polymorphisms to clinical findings in overactive bladder. World J Urol 38, 2571–2575 (2020). https://doi.org/10.1007/s00345-019-03046-5

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Keywords

  • Polymorphism
  • Overactive bladder
  • Smooth muscle
  • OAB score