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Autologous fibroblast transplantation at the vesico-ureteral junction as potential reconstructive cell replacement in an animal model

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Abstract

Purpose

To evaluate the cellular survival of donor fibroblasts after transplantation at the vesico-ureteral junction (VUJ) and to analyse their potential for reconstructive cell replacement in an animal model as autologous fibroblasts have been used as soft tissue augmentation material for scared and damaged tissue.

Methods

Muscles biopsies were procured from the lower limb muscles of 4 pigs; cytoplasm of fibroblasts was labelled with nano-sized iron oxide particles. Six weeks after taking of the muscle biopsies, fibroblast transplantation was performed, 3 × 106 cells suspended in transplantation medium (in 1-ml syringes) were injected at the VUJ using the modified STING technique. Animals were killed 8 weeks later; seeded fibroblasts were identified using prussian blue staining protocol; histological evaluation and morphological analysis were performed by light microscopy (Mayer’s haematoxylin-eosin staining); and bladders were scanned by MRI for visualization and localization of the iron-labelled donor cells.

Results

Donor fibroblast cell colonization and cellular viability at the VUJ was demonstrated by MRI and histochemically indicating cellular uptake of iron particles at the VUJ. It was also evident that transplanted fibroblasts integrate into the extracellular matrix of the distal ureter augmenting ureteral host tissue.

Conclusions

Labelled implanted autologous fibroblasts were visualized by staining procedure as well as MRI scan demonstrating persistence at the VUJ, suggesting that in vitro expanded fibroblasts survived in vivo after transplantation.

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Acknowledgments

We would like to sincerely thank each and every reviewer who dedicated his or her time and expertise to reviewing our manuscripts and everybody who supported us and collaborated with us.

Conflict of interest

The authors declare that they have no conflicts of interest.

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Authors and Affiliations

  1. Department of Urology, Medical University of Innsbruck, Anichstreet 35, 6020, Innsbruck, Austria

    Renate Pichler, Viktor Skradski, Wolfgang Horninger & Josef Oswald

  2. Department of Anatomy, Histology und Embryology, Medical University of Innsbruck, Muellerstrasse 59, 6020, Innsbruck, Austria

    Elisabeth Richter

  3. Division of Histology and Embryology, Medical University of Innsbruck, Muellerstrasse 59, 6020, Innsbruck, Austria

    Guenter Klima

  4. Department of Anaesthesia, Medical University of Innsbruck, Anichstreet 35, 6020, Innsbruck, Austria

    Viktoria Mayr

  5. Innovacell biotechnology, Mitterweg 24, 6020, Innsbruck, Austria

    Rainer Marksteiner

Authors
  1. Renate Pichler
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  2. Guenter Klima
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  3. Elisabeth Richter
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  4. Rainer Marksteiner
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  5. Viktoria Mayr
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  6. Viktor Skradski
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  7. Wolfgang Horninger
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  8. Josef Oswald
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Corresponding author

Correspondence to Josef Oswald.

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Pichler, R., Klima, G., Richter, E. et al. Autologous fibroblast transplantation at the vesico-ureteral junction as potential reconstructive cell replacement in an animal model. World J Urol 31, 169–174 (2013). https://doi.org/10.1007/s00345-012-0914-1

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  • DOI: https://doi.org/10.1007/s00345-012-0914-1

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