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GSTP1 CpG island hypermethylation for DNA-based detection of occult tumor cells in surgical margins after radical prostatectomy

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Abstract

Objective

The risk of local recurrence after radical prostatectomy (RP) is considerably dependent on local tumor stage. To improve local staging, the aim of this study was to assess the feasibility of quantitative methylation-specific PCR (Q-MSP) for the identification of promoter hypermethylation of the detoxifying glutathione-S-transferase P1 gene (GSTP1) to detect occult prostate cancer (PCa) cells in the prostatic fossa after RP.

Methods

A total of 39 consecutive patients with clinically organ-confined PCa underwent RP. After gland excision, biopsies were obtained from eight defined areas of the prostatic fossa and bisected for both histopathological and molecular analyses. Results were related to clinicopathological data including tumor stage, Gleason score, prostate-specific antigen (PSA), and biochemical recurrence.

Results

Of 39 patients, 11 with PCa had at least one positive molecular margin status indicated by GSTP1 methylation. These included 5 of 17 (29.4%) with organ-confined and 6 of 22 (27.3%) with advanced (≥pT3 and/or pN+) PCa. GSTP1 methylation in surgical margins strongly correlated with histopathological R-status (P = 0.022) and preoperative PSA (P = 0.01) whereas no association with tumor stage (pT2 vs pT3), grade (Gleason score <7 vs ≥7), and lymph node status was found. No patient experienced biochemical relapse.

Conclusions

GSTP1 hypermethylation detected by Q-MSP in prostatic fossa biopsies after RP is well suited for the detection of occult tumor cells in surgical margins. However, the limited number of patients and the short-term follow-up does not allow definite conclusions on the prognostic value of GSTP1 in surgical margins.

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Acknowledgments

The authors are very grateful to the skillful technical assistance of Mrs. Waltraud Jekabsons.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Urology, University Hospital Ulm, Prittwitzstrasse 43, 89075, Ulm, Germany

    Florian Jentzmik, Andres Jan Schrader & Mark Schrader

  2. Department of Urology, Charité Hospital Berlin, University Medicine Berlin, Berlin, Germany

    Hans Krause, Daniel Baumunk, Hannes Cash, Kurt Miller & Martin Schostak

  3. Department of Pathology, Charité Hospital Berlin, University Medicine Berlin, Berlin, Germany

    Ute Reichelt

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  1. Florian Jentzmik
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  2. Hans Krause
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  3. Ute Reichelt
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  4. Andres Jan Schrader
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  6. Daniel Baumunk
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  7. Hannes Cash
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  8. Kurt Miller
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  9. Martin Schostak
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Correspondence to Florian Jentzmik.

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Jentzmik, F., Krause, H., Reichelt, U. et al. GSTP1 CpG island hypermethylation for DNA-based detection of occult tumor cells in surgical margins after radical prostatectomy. World J Urol 30, 541–546 (2012). https://doi.org/10.1007/s00345-011-0764-2

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  • DOI: https://doi.org/10.1007/s00345-011-0764-2

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