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Upregulation of mRNA expression of MCP-1 by TGF-β1 in fibroblast cells from Peyronie’s disease

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Abstract

Introduction

Peyronie’s disease (PD) is a localized connective tissue disorder of the penile tunica albuginea (TA) with a still obscure etiopathology. Recent studies from our laboratory have demonstrated differences in Smad3 and Smad4 gene expression of PD-fibroblasts and non-PD-fibroblasts after stimulation with recombinant TGF-β1 for 1 h. In the present study, we investigated gene expression of Smad2–Smad4 and Smad7 up to 6 h after stimulation with TGF-β1. As a positive control, MCP-1 gene expression was monitored.

Materials and methods

Cells with fibroblast characteristics were isolated from seven PD plaques and three TA controls. The cells were incubated with recombinant TGF-β1 for 2–6 h and expression of Smad2–Smad4, Smad7, and monocyte chemotactic protein-1 (MCP-1) was determined by quantitative real-time PCR.

Results

TGF-β1 treatment resulted in a statistically significant up-regulation of Smad7 and MCP-1 gene expression. Smad7 expression was increased after 2 h (P < 0.001) and was still high after 4 h (P < 0.05). No significant differences between fibroblasts from PD-patients compared to non-PD-patients were observed. MCP-1 peaked after 4 h (P < 0.001) and remained high up to 6 h (P < 0.01). PD-fibroblasts revealed a significantly increased MCP-1 gene expression compared to non-PD-fibroblasts (P = 0.013) after 2 h and remained significantly different also after 6 h (P = 0.038). Gene expression of Smad2–Smad4 did not change during stimulation with TGF-β1.

Conclusion

In conclusion, analysis of MCP-1 expression might be a useful marker for Peyronie’s disease.

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Authors and Affiliations

  1. Department of Urology, Medical Faculty, University Hospital Giessen, Justus Liebig University, Rudolf-Buchheim-Str. 7, 35392, Giessen, Germany

    Carolin Szardening-Kirchner, Ekkehard W. Hauck, Simone M. Haag & Wolfgang Weidner

  2. Department of Obstetrics and Gynecology, Medical Faculty, University Hospital Giessen, Justus Liebig University, Klinikstr. 32, 35392, Giessen, Germany

    Lutz Konrad

  3. Department of Medicine II, Medical Faculty, University Hospital Giessen, Justus Liebig University, Aulweg 123, 35392, Giessen, Germany

    Oliver Eickelberg

Authors
  1. Carolin Szardening-Kirchner
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  2. Lutz Konrad
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  3. Ekkehard W. Hauck
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  4. Simone M. Haag
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  5. Oliver Eickelberg
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  6. Wolfgang Weidner
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Corresponding author

Correspondence to Lutz Konrad.

Additional information

C. Szardening-Kirchner and L. Konrad have contributed equally to the study.

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Szardening-Kirchner, C., Konrad, L., Hauck, E.W. et al. Upregulation of mRNA expression of MCP-1 by TGF-β1 in fibroblast cells from Peyronie’s disease. World J Urol 27, 123–130 (2009). https://doi.org/10.1007/s00345-008-0320-x

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  • DOI: https://doi.org/10.1007/s00345-008-0320-x

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