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Elucidating the Defence Response of Wheat Mutants Developed for augmenting Terminal Heat Stress Tolerance and Improved Grain-Quality

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Abstract

Terminal heat stress is one of the major problems in wheat growth, yield and grain-quality. Here, we have developed wheat mutant (M3) for HS-tolerance and improved grain-quality using gamma irradiation [parent-MP3054- C-306/CB.SPRING BW/CPAN2072 (Parentage)]. Biochemical markers based characterization showed wheat mutant to be better in HS-tolerance and grain-quality, as compared to parent. To elucidate the mechanism of thermotolerance in wheat mutant, we performed de novo transcriptome assembly of mutant (M3), parent (P3), and mutant exposed to HS (M3H). We generated 6.3 (P3-R1), 6.6 (P3-R2), 8.1 (M3-R1), 7.1 (M3-R2), 7.6 (M3H-R1), and 6.1 (M3H-R2) million clean reads and identified 3,05,537 genes and 5,88,788 transcripts with an N50 of 1,349 bp. We observed 6,120 upregulated and 4,428 downregulated transcripts (M3 vs P3), 11,354 upregulated and 12,408 downregulated genes (M3H vs P3) and 4817 upregulated and 9085 downregulated genes (M3H vs M3). The high HS-tolerance and improved grain-quality of the wheat mutant was observed due to the upregulation of serine threonine kinase (STK), HSP20, SOD, ATP-binding cassette (ABC) transporters, heat shock transcription factor (HSF), and calcium dependent protein kinase (CDPK) and stability of starch synthase, sucrose synthase, and debranching enzyme. Gene Ontology analysis showed ‘ATP-binding’ to be most enriched category. The carbon assimilatory pathways (photosynthesis and starch biosynthesis) were observed most altered under terminal HS. The developed mutant can be further utilized as donor in wheat breeding program to develop ‘climate-smart’ crop.

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Data Availability

The data sets generated and analysed during the current study are available in the NCBI BioProject Db repository with accession no PRJNA551093 (https://www.ncbi.nlm.nih.gov/bioproject/PRJNA551093).

Abbreviations

HSP:

Heat shock protein

DETs:

Differentially expressed transcripts

DEGs:

Differentially expressed genes

STKs:

Serine threonine kinases

SOD:

Superoxide dismutase

GO:

Gene ontology

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Acknowledgements

Authors duly acknowledge the Bhabha Atomic Research Center (BARC), Mumbai for providing the seeds of wheat mutant developed for the HS-tolerance. This work was supported by Board of Research in Nuclear Sciences (BRNS) (sanction no 35/14/18/2017) and Indian Council of Agricultural Research (ICAR) under National Innovations on Climate Resilient Agriculture (NICRA) project (Sanction no. TG-3079).

Funding

This work was supported by Board of Research in Nuclear Sciences (BRNS) [sanction no 35/14/18/2017] and Indian Council of Agricultural Research (ICAR) under National Innovations on Climate Resilient Agriculture (NICRA) project [Sanction no. TG-3079].

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Contributions

R.R.K., S.P. conceptualize the idea and plan the experiment; S.B., S.J.J. developed the wheat mutant using gamma irradiation and evaluated the mutants in different generations; S.B., S.N.K., B.S. performed the genetic and physiological characterization of mutants; R.R.K., G.P.M., G.K.R., S.P. planned and executed the de novo transcriptome sequencing; R.R.K., V.T., G.P.S. performed the bioinformatics characterization of generated data; S.H., S.K., S.G. evaluated the mutant and parent for the tolerance level and grain-quality; K.D., S.P. performed the expression analysis of DEGs and scatter plot analysis; R.R.K., V.C., S.P. drafted the manuscript and edited the language.

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Correspondence to Ranjeet R. Kumar or Shelly Praveen.

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The authors have no competing interests to declare that are relevant to the content of this article.

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Handling Author: Golam Jalal Ahammed.

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Kumar, R.R., Bakshi, S., Goswami, S. et al. Elucidating the Defence Response of Wheat Mutants Developed for augmenting Terminal Heat Stress Tolerance and Improved Grain-Quality. J Plant Growth Regul (2024). https://doi.org/10.1007/s00344-024-11355-2

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  • DOI: https://doi.org/10.1007/s00344-024-11355-2

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